Rabbit Recombinant Monoclonal HDGF antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-HDGF antibody [EPR7899] - BSA and Azide free (AB248356), expandable thumbnail](https://content.abcam.com/products/images/hdgf-antibody-epr7899-bsa-and-azide-free-ab248356--western-blot-img116346.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HDGF antibody [EPR7899] - BSA and Azide free (AB248356)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDGF antibody [EPR7899] - BSA and Azide free (AB248356), expandable thumbnail](https://content.abcam.com/products/images/hdgf-antibody-epr7899-bsa-and-azide-free-ab248356--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img61226.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDGF antibody [EPR7899] - BSA and Azide free (AB248356)")
![Immunocytochemistry/ Immunofluorescence - Anti-HDGF antibody [EPR7899] - BSA and Azide free (AB248356), expandable thumbnail](https://content.abcam.com/products/images/hdgf-antibody-epr7899-bsa-and-azide-free-ab248356--immunocytochemistry-immunofluorescence-img88461.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-HDGF antibody [EPR7899] - BSA and Azide free (AB248356)")
![Flow Cytometry (Intracellular) - Anti-HDGF antibody [EPR7899] - BSA and Azide free (AB248356), expandable thumbnail](https://content.abcam.com/products/images/hdgf-antibody-epr7899-bsa-and-azide-free-ab248356--flow-cytometry-intracellular-img164800.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-HDGF antibody [EPR7899] - BSA and Azide free (AB248356)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | WB | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Isoform 1. Acts as a transcriptional repressor (PubMed:17974029). Has mitogenic activity for fibroblasts (PubMed:11751870, PubMed:26845719). Heparin-binding protein (PubMed:15491618). Isoform 2. Does not have mitogenic activity for fibroblasts (PubMed:26845719). Does not bind heparin (PubMed:26845719). Isoform 3. Has mitogenic activity for fibroblasts (PubMed:26845719). Heparin-binding protein (PubMed:26845719).
Alternative names
HMG1L2, HDGF, Hepatoma-derived growth factor, High mobility group protein 1-like 2, HMG-1L2
Recommended products
Rabbit Recombinant Monoclonal HDGF antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR7899
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248356 is the carrier-free version of Anti-HDGF antibody [EPR7899] ab131046.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Hepatoma-Derived Growth Factor commonly referred to as HDGF or HDGF protein (UniProt ID: K562) operates as a heparin-binding protein with a mass of about 26 kDa. It plays a significant role in cell growth regulation and development processes. HDGF is expressed in the liver and various other tissues including the heart and lungs. It contains an N-terminal PWWP domain which is important for its binding properties and influences its interaction with other molecules.
- Biological function summary
HDGF affects cellular processes such as proliferation and differentiation. It does not function as part of a larger protein complex but is known to modulate transcriptional activities within the cell. HDGF's mitogenic properties suggest it can promote tumor progression by influencing the cell's growth and survival mechanisms indicating its involvement in oncogenic pathways.
- Pathways
HDGF is integrated into important signaling networks such as the MAPK/ERK pathway. This pathway is important for transmitting signals from the cell surface to the DNA in the cell nucleus affecting cell division and fate. HDGF interacts with proteins in the pathways such as ERK1/2 which are critical regulators of cellular responses. HDGF’s involvement in these pathways indicates its role in mediating cell growth signals and adjusting the cellular response to environmental changes.
- Associated diseases and disorders
HDGF has associations with cancer progression including hepatocellular carcinoma and lung cancer. Its overexpression correlates with poor prognosis in these malignancies due to its role in enhancing tumor cell proliferation and resistance to apoptosis. HDGF's relationship with disease involves its potential interaction with oncogenic proteins like Ras which can activate downstream signaling cascades contributing to tumorigenesis. Understanding HDGF's role in disease can further research into targeted therapies aimed at mitigating its effects on cancer progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-HDGF antibody [EPR7899] - BSA and Azide free (ab248356), expandable thumbnail](https://content.abcam.com/products/images/hdgf-antibody-epr7899-bsa-and-azide-free-ab248356--western-blot-img116346.jpg "Western blot - Anti-HDGF antibody [EPR7899] - BSA and Azide free (ab248356)")
Western blot - Anti-HDGF antibody [EPR7899] - BSA and Azide free (ab248356)
This data was developed using Anti-HDGF antibody [EPR7899] ab131046, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-HDGF antibody [EPR7899] (Anti-HDGF antibody [EPR7899] ab131046) at 1/10000 dilution
Lane 1: A549 cell lysate at 10 µg
Lane 2: Saos-2 cell lysate at 10 µg
Lane 3: ECV-304 cell lysate at 10 µg
Lane 4: 293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 27 kDa
Observed band size: 40 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDGF antibody [EPR7899] - BSA and Azide free (ab248356), expandable thumbnail](https://content.abcam.com/products/images/hdgf-antibody-epr7899-bsa-and-azide-free-ab248356--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img61226.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDGF antibody [EPR7899] - BSA and Azide free (ab248356)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDGF antibody [EPR7899] - BSA and Azide free (ab248356)
This data was developed using Anti-HDGF antibody [EPR7899] ab131046, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling HDGF with Anti-HDGF antibody [EPR7899] ab131046 antibody at 1/250 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-HDGF antibody [EPR7899] - BSA and Azide free (ab248356), expandable thumbnail](https://content.abcam.com/products/images/hdgf-antibody-epr7899-bsa-and-azide-free-ab248356--immunocytochemistry-immunofluorescence-img88461.jpg "Immunocytochemistry/ Immunofluorescence - Anti-HDGF antibody [EPR7899] - BSA and Azide free (ab248356)")
Immunocytochemistry/ Immunofluorescence - Anti-HDGF antibody [EPR7899] - BSA and Azide free (ab248356)
This data was developed using Anti-HDGF antibody [EPR7899] ab131046, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of A549 cells labelling HDGF with Anti-HDGF antibody [EPR7899] ab131046 antibody at 1/250 dilution.
![Flow Cytometry (Intracellular) - Anti-HDGF antibody [EPR7899] - BSA and Azide free (ab248356), expandable thumbnail](https://content.abcam.com/products/images/hdgf-antibody-epr7899-bsa-and-azide-free-ab248356--flow-cytometry-intracellular-img164800.jpg "Flow Cytometry (Intracellular) - Anti-HDGF antibody [EPR7899] - BSA and Azide free (ab248356)")
Flow Cytometry (Intracellular) - Anti-HDGF antibody [EPR7899] - BSA and Azide free (ab248356)
This data was developed using Anti-HDGF antibody [EPR7899] ab131046, the same antibody clone in a different buffer formulation.
Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labeling HDGF with purified Anti-HDGF antibody [EPR7899] ab131046 at 1/1000 dilution (1 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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