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AB327016

Anti-HDGFL2 cryptic peptide antibody [EPR31252-576] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal HRP2 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

HDGF2, HDGFRP2, HRP2, UNQ785/PRO1604, HDGFL2, Hepatoma-derived growth factor-related protein 2, HDGF-related protein 2, HRP-2, Hepatoma-derived growth factor 2, HDGF-2

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Sandwich ELISA - Anti-HDGFL2 cryptic peptide antibody [EPR31252-576] - BSA and Azide free (Detector) (AB327016)
  • sELISA

Lab

Sandwich ELISA - Anti-HDGFL2 cryptic peptide antibody [EPR31252-576] - BSA and Azide free (Detector) (AB327016)

Sandwich ELISA with the capture antibody ab327015 dilution at 2 µg/mL and detector antibody ab327016 dilution at 0.5 µg/mL after biotin conjugation.

Sandwich ELISA - Anti-HDGFL2 cryptic peptide antibody [EPR31252-576] - BSA and Azide free (Detector) (AB327016)
  • sELISA

Lab

Sandwich ELISA - Anti-HDGFL2 cryptic peptide antibody [EPR31252-576] - BSA and Azide free (Detector) (AB327016)

Interpolated concentration of native HDGFL2 cryptic peptide was measured at different sample concentrations. Undiluted samples are as follows : TARDBP knockout HAP1 cell extract (125 µg/mL) and wild type HAP1 cell extract (125 µg/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Interpolated concentrations of HDGFL2 cryptic peptide in wild type HAP1 cell extract were below the detectable range of the assay. Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR31252-576

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

<p>This antibody recognizes HDGFL2 cryptic peptide and shows no cross-reactivity with HDGFL2 wild-type. The antibody pair, consisting of ab327015 as the capture and ab327016 as the detector, exhibits no cross-reactivity with HDGFL2 wild-type.</p>

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

ab327016 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human HDGFL2. The recommended pair for sandwich ELISA is:

Capture: ab327015, Human HDGFL2 Capture Antibody (unconjugated)

Detector: ab327016, Human HDGFL2 Detector Antibody (unconjugated)

The reference range value is 156-5000 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as an epigenetic regulator of myogenesis in cooperation with DPF3a (isoform 2 of DPF3/BAF45C) (PubMed : 32459350). Associates with the BAF complex via its interaction with DPF3a and HDGFL2-DPF3a activate myogenic genes by increasing chromatin accessibility through recruitment of SMARCA4/BRG1/BAF190A (ATPase subunit of the BAF complex) to myogenic gene promoters (PubMed : 32459350). Promotes the repair of DNA double-strand breaks (DSBs) through the homologous recombination pathway by facilitating the recruitment of the DNA endonuclease RBBP8 to the DSBs (PubMed : 26721387). Preferentially binds to chromatin regions marked by H3K9me3, H3K27me3 and H3K36me2 (PubMed : 26721387, PubMed : 32459350). Involved in cellular growth control, through the regulation of cyclin D1 expression (PubMed : 25689719).
See full target information HDGFL2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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