JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB247836

Anti-HDLBP antibody [EPR5267] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal HDLBP antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.

View Alternative Names

HBP, VGL, HDLBP, Vigilin, High density lipoprotein-binding protein, HDL-binding protein

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDLBP antibody [EPR5267] - BSA and Azide free (AB247836)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDLBP antibody [EPR5267] - BSA and Azide free (AB247836)

This data was developed using ab109324, the same antibody clone in a different buffer formulation.

ab109324, at a 1/100 dilution, staining HDLBP in paraffin embedded Human stomach tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-HDLBP antibody [EPR5267] - BSA and Azide free (AB247836)
  • WB

Unknown

Western blot - Anti-HDLBP antibody [EPR5267] - BSA and Azide free (AB247836)

This data was developed using ab109324, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HDLBP antibody [EPR5267] (<a href='/en-us/products/primary-antibodies/hdlbp-antibody-epr5267-ab109324'>ab109324</a>) at 1/10000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 141 kDa

Observed band size: 155 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5267

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column

Product details

ab247836 is the carrier-free version of ab109324.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The HDLBP also known as Vigilin is a protein that interacts with high-density lipoproteins (HDL). It has a molecular mass of approximately 116 kDa. HDLBP plays an important role in binding RNA and is largely expressed in the liver and adipose tissues. Through its RNA-binding capacity HDLBP regulates the stability and translation of specific mRNAs impacting various cellular processes.
Biological function summary

Without HDLBP's regulatory functions in RNA metabolism cells struggle to maintain proper gene expression. This protein is not known to operate as part of a multi-protein complex but acts independently to influence RNA molecule interactions. HDLBP regulates lipid metabolism by facilitating the transport and stabilization of RNA species that encode enzymes important for lipid synthesis and uptake.

Pathways

The RNA-binding functions of HDLBP integrate into lipid metabolism and transport pathways. In the lipid metabolic pathway HDLBP interacts with other proteins such as apolipoproteins to manage lipid homeostasis. In addition it plays a part in the RNA processing and transport pathway linking its activity to proteins involved in RNA export and translation control.

Without balanced HDLBP activity individuals show an increased risk of developing atherosclerosis and metabolic syndrome. The improper regulation of HDL and lipid metabolism contributes to these conditions. HDLBP's role overlaps with the functions of apolipoprotein A-I in these disorders as both participate in managing HDL-cholesterol levels and lipid homeostasis. Understanding HDLBP's mechanisms can help target therapeutic strategies for cardiovascular diseases.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Appears to play a role in cell sterol metabolism. It may function to protect cells from over-accumulation of cholesterol.
See full target information HDLBP
style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB65390

Cholesterol Assay Kit - HDL and LDL/VLDL

Biochemical assay - Cholesterol Assay Kit - HDL and LDL/VLDL (AB65390)

  • 4
  • 10
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Primary Antibodies

AB183218

Anti-TNF alpha antibody [EPR19147]

KO Validated|RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [EPR19147] (AB183218)

  • 5
  • 1
Primary Antibodies

AB66217

Anti-ABCA1 antibody [HJ1]

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA1 antibody [HJ1] (AB66217)

  • 3
  • 3
Primary Antibodies

AB233706

Anti-IL-6 antibody [EPR21711]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-IL-6 antibody [EPR21711] (AB233706)

  • 5
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com