Rabbit Polyclonal HE4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human WFDC2.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human WFDC2. Database link Q14508
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Alternative names
HE4, WAP5, WFDC2, WAP four-disulfide core domain protein 2, Epididymal secretory protein E4, Major epididymis-specific protein E4, Putative protease inhibitor WAP5
Recommended products
Rabbit Polyclonal HE4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human WFDC2.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human WFDC2. Database link Q14508
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Human Epididymis Protein 4 (HE4) also known as WAP four-disulfide core domain protein 2 (WFDC2) is a small glycoprotein of approximately 13 kDa. HE4 is most highly expressed in the epithelial cells of reproductive tissues like the epididymis but also in tissues such as the respiratory and urogenital tracts. HE4 is secreted into the extracellular space where it functions and is found in normal range in different body fluids. This secreted protein has gained attention due to its use in various diagnostic tests such as the HE4 test and HE4 assay utilizing HE4 antibodies.
- Biological function summary
HE4 participates in innate immune response and epithelial cell protection. HE4 protein plays a role in the modulation of antiprotease activity as it is involved in inhibiting serine proteases. It is not part of a larger protein complex but interacts with multiple protease signaling pathways. HE4 is often studied for its role in modulating protein activity at epithelial barriers contributing to tissue homeostasis and protective mechanisms against infections.
- Pathways
HE4 interacts within signaling cascades such as the innate immune pathways and extracellular matrix organization. It links to proteins within these pathways like serine proteases and other WFDC family proteins. HE4 influences protease activity regulation an essential component of tissue remodeling and inflammatory response impacting overall pathway dynamics.
- Associated diseases and disorders
HE4 has strong associations with ovarian cancer and endometrial cancer. Its overexpression in these malignancies makes it a valuable biomarker for disease diagnosis and prognosis. HE4 interacts with cancer-related proteins such as CA125 enhancing its utility in combinatory diagnostic approaches. The HE4 test and HE4 ELISA kit price underline its significance in clinical settings for monitoring these cancers where timely detection influences treatment outcomes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Western blot - Anti-HE4 antibody (ab273130)
False colour image of Western blot: Anti-HE4 antibody staining at 0.04 ug/ml, shown in black; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab273130 was shown to bind specifically to HE4. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with Optiblot (ECL reagent Anti-PKC delta (phospho S299) antibody [EPNCI119] ab133456) and imaged with 4 minutes exposure time. Secondary antibodies used were HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
All lanes: Western blot - Anti-HE4 antibody (ab273130) at 0.04 µg/mL
Lane 1: OVCAR-3 cell lysate at 20 µg
Lane 2: K562 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 13 kDa
Observed band size: 20 kDa
Immunocytochemistry/ Immunofluorescence - Anti-HE4 antibody (ab273130)
PFA-fixed, Triton X-100-permeabilized A431 (Human epidermoid carcinoma cell line) cells stained for HE4 using ab273130 at 2 μg/ml (green) in ICC/IF.
Western blot - Anti-HE4 antibody (ab273130)
All lanes: Western blot - Anti-HE4 antibody (ab273130) at 0.4 µg/mL
All lanes: EFO-21 (Human cell line) cell lysate
Predicted band size: 13 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HE4 antibody (ab273130)
Paraffin-embedded human cervix (uterine) tissue stained for HE4 using ab273130 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HE4 antibody (ab273130)
Paraffin-embedded human ovary tissue stained for HE4 using ab273130 at 1/50 dilution in immunohistochemical analysis.
Shows low expression as expected.
Retrieval method: HIER pH6.
Western blot - Anti-HE4 antibody (ab273130)
False colour image of Western blot: Anti-HE4 antibody staining at 0.4 ug/ml, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab273130 was shown to bind specifically to HE4. A band was observed at 20 kDa in treated wild-type OVCAR-3 cell lysates with no signal observed at this size in WFDC2 knockout cell line ab275410 (knockout cell lysate ab275536). To generate this image, wild-type and WFDC2 knockout OVCAR-3 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-HE4 antibody (ab273130) at 0.4 µg/mL
Lane 1: Wild-type OVCAR-3 Treated Brefeldin A (Brefeldin A Solution (1,000X) ab193369) (5 ug/mL, 4 h), Monensin (Monensin Solution (1,000X) ab193381) (2 uM, 4 h) cell lysate at 20 µg
Lane 2: Wild-type OVCAR-3 Vehicle Control Brefeldin A (Brefeldin A Solution (1,000X) ab193369) (0 ug/mL, 4 h), Monensin (Monensin Solution (1,000X) ab193381) (0 uM, 4 h) cell lysate at 20 µg
Lane 3: WFDC2 knockout OVCAR-3 Treated Brefeldin A (Brefeldin A Solution (1,000X) ab193369) (5 ug/mL, 4 h), Monensin (Monensin Solution (1,000X) ab193381) (2 uM, 4 h) cell lysate at 20 µg
Lane 4: WFDC2 knockout OVCAR-3 Vehicle Control Brefeldin A (Brefeldin A Solution (1,000X) ab193369) (0 ug/mL, 4 h), Monensin (Monensin Solution (1,000X) ab193381) (0 uM, 4 h) cell lysate at 20 µg
Performed under reducing conditions.
Observed band size: 20 kDa
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