AB243102

Anti-Heat-labile enterotoxin B chain antibody

Name: Anti-Heat-labile enterotoxin B chain antibody (ab243102) | Abcam
Brand: Abcam Limited
SKU: AB243102
Price: 520 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Heat-labile enterotoxin B chain antibody. Suitable for ELISA, Dot, WB and reacts with Clostridium perfringens, Purified native protein - Clostridium perfringens samples. Cited in 1 publication. Immunogen corresponding to Native Full Length Protein corresponding to Clostridium perfringens Heat-labile enterotoxin B chain.

View Alternative Names

Heat-labile enterotoxin B chain, cpe

2 Images

Supplier Data

Western blot - Anti-Heat-labile enterotoxin B chain antibody (AB243102)

All lanes:

Anti-MFF antibody (<a href='/en-us/products/unavailable/mff-antibody-ab242102'>ab242102</a>) at 1 µg/mL

All lanes:

Crude extract of Clostridium perfringens

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 35 kDa

false

Supplier Data

Dot Blot - Anti-Heat-labile enterotoxin B chain antibody (AB243102)

Dot blot of highly purified C.perfringence enterotoxin (antigen) using ab243102 at the indicated concentrations. ab97051 was used as the secondary antibody at 1/20,000 dilution for 60 minutes.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Clostridium perfringens

Applications

WB, ELISA, Dot

applications

Immunogen

Native Full Length Protein corresponding to Clostridium perfringens Heat-labile enterotoxin B chain.

P01558

Specificity

Clostridium perfringens enterotoxin.(food-poisoning strains).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Clostridium perfringens": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Purified native protein - Clostridium perfringens": { "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/100000 - 1/200000", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 6.5 - 8.5 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Heat-labile enterotoxin B chain (LT-B) also known as Escherichia coli heat-labile enterotoxin subunit B plays an important role in the functioning of certain bacterial toxins. LT-B with a mass of approximately 11.6 kDa forms a pentameric structure. It expresses in specific strains of Escherichia coli often within the gastrointestinal tract. The B chain helps the toxin bind to host cells by interacting with the GM1 ganglioside receptors on the surface of epithelial cells facilitating the entry of the catalytic A subunit into the cell.

Biological function summary

LT-B acts as a non-toxic subunit that enables the binding of the toxin complex to target cells. It forms a pentamer a stable ring structure which contributes to the adherence of bacteria to intestinal mucosa. While LT-B itself is not toxic it is essential for the delivery of the toxic A chain which exerts the physiological effects of the toxin. The entire enterotoxin complex plays a part in increasing intestinal secretion resulting in symptoms such as diarrhea.

Pathways

LT-B facilitates the activation of signaling events in the host cell that lead to increased cyclic AMP levels via the adenylate cyclase pathway. This activation disrupts normal cellular ionic balances promoting fluid and electrolyte movement into the intestinal lumen. The pathways involving LT-B closely link to proteins such as GM1 ganglioside and adenylate cyclase coordinating to amplify the effects of the enterotoxin A subunit in signal transduction.

LT-B significantly contributes to conditions like traveler's diarrhea and other infections caused by enterotoxigenic Escherichia coli (ETEC). The B subunit's mediative properties of cell entry are important for these pathologies exacerbating the symptoms by enhancing toxin delivery and action. In the context of its involvement in disease the interaction with the A chain is fundamental as the A subunit's enzymatic activity results in the pathological hypersecretion observed in cases of ETEC infection.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This enterotoxin is responsible for many cases of a mild type of food poisoning.

See full target information Heat-labile enterotoxin B chain

Additional targets

Heat-labile enterotoxin B chain

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Microbiology spectrum 11:e0152523 PubMed37916813

2023

ArcA positively regulates the expression of virulence genes and contributes to virulence of porcine Shiga toxin-producing enterotoxigenic .

Applications

Unspecified application

Species

Unspecified reactive species

Fengwei Jiang,Yan Yang,Zhao Mao,Wentong Cai,Ganwu Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Heat-labile enterotoxin B chain antibody

Western blot - Anti-Heat-labile enterotoxin B chain antibody (AB243102)

Dot Blot - Anti-Heat-labile enterotoxin B chain antibody (AB243102)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (1)

Product promise