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AB122762

Anti-HEATR3 antibody

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Rabbit Polyclonal HEATR3 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HEATR3 aa 550 to C-terminus.

View Alternative Names

HEAT repeat-containing protein 3, HEATR3

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEATR3 antibody (AB122762)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEATR3 antibody (AB122762)

ab122762, at 1/1000 dilution, staining HEATR3 in paraffin-embedded Human bone marrow tissue by Immunohistochemistry.

Immunocytochemistry/ Immunofluorescence - Anti-HEATR3 antibody (AB122762)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HEATR3 antibody (AB122762)

Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus, nucleoli and cytoplasm. Recommended concentration of ab122762 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human HEATR3 aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q7Z4Q2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "<p>Recommend PFA Fixation and Triton X-100 treatment</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HEATR3 also known as HEAT repeat-containing protein 3 is a protein characterized by HEAT repeats which are tandemly repeated motifs facilitating protein-protein interactions. This protein has a mass of approximately 94 kDa. Researchers have identified its expression in various tissues suggesting its broad role in cellular function. The structural design of HEATR3 implies it might act as a molecular scaffold helping in processes that need complex assemblies.
Biological function summary

HEATR3 assists in translation machinery modulation. It forms part of the R2TP/Prefoldin-like complex a multi-protein complex engaged in the assembly and maturation of large protein complexes. This involvement implies an important role in maintaining the stability and function of essential cellular structures. Additionally HEATR3 interacts with proteins containing HEAT repeats hinting at remarkable versatility in cellular tasks.

Pathways

HEATR3 is important in the process of ribosome biogenesis and cellular stress response pathways. It works closely with the PI3K/AKT signaling pathway aiding in the regulation of growth and survival signals. The interaction with related pathway proteins such as PIH1D1 indicates HEATR3's participation in cellular systems affecting proliferation and apoptosis.

HEATR3 expression and function appear linked to cancer and neurodegenerative diseases. Abnormal expression patterns or mutations in HEATR3 might contribute to the pathogenesis of specific cancer types potentially through disrupted interactions with MTOR or other cancer-related proteins. Additionally the protein's role in proper cellular assembly and stress response implicates it in neurodegenerative disorders where these processes are often impaired.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in ribosome biogenesis and in nuclear import of the 60S ribosomal protein L5/large ribosomal subunit protein uL18 (RPL5) (PubMed : 35213692). Required for proper erythrocyte maturation (PubMed : 35213692).
See full target information HEATR3
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Product promise

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