Rabbit Polyclonal HEATR3 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HEATR3 aa 550 to C-terminus.
IgG
Rabbit
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
IHC-P | ICC/IF | |
---|---|---|
Human | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/2500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1.00000-4.00000 µg/mL | Notes Recommend PFA Fixation and Triton X-100 treatment |
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Plays a role in ribosome biogenesis and in nuclear import of the 60S ribosomal protein L5/large ribosomal subunit protein uL18 (RPL5) (PubMed:35213692). Required for proper erythrocyte maturation (PubMed:35213692).
HEAT repeat-containing protein 3, HEATR3
Rabbit Polyclonal HEATR3 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HEATR3 aa 550 to C-terminus.
IgG
Rabbit
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
HEATR3 also known as HEAT repeat-containing protein 3 is a protein characterized by HEAT repeats which are tandemly repeated motifs facilitating protein-protein interactions. This protein has a mass of approximately 94 kDa. Researchers have identified its expression in various tissues suggesting its broad role in cellular function. The structural design of HEATR3 implies it might act as a molecular scaffold helping in processes that need complex assemblies.
HEATR3 assists in translation machinery modulation. It forms part of the R2TP/Prefoldin-like complex a multi-protein complex engaged in the assembly and maturation of large protein complexes. This involvement implies an important role in maintaining the stability and function of essential cellular structures. Additionally HEATR3 interacts with proteins containing HEAT repeats hinting at remarkable versatility in cellular tasks.
HEATR3 is important in the process of ribosome biogenesis and cellular stress response pathways. It works closely with the PI3K/AKT signaling pathway aiding in the regulation of growth and survival signals. The interaction with related pathway proteins such as PIH1D1 indicates HEATR3's participation in cellular systems affecting proliferation and apoptosis.
HEATR3 expression and function appear linked to cancer and neurodegenerative diseases. Abnormal expression patterns or mutations in HEATR3 might contribute to the pathogenesis of specific cancer types potentially through disrupted interactions with MTOR or other cancer-related proteins. Additionally the protein's role in proper cellular assembly and stress response implicates it in neurodegenerative disorders where these processes are often impaired.
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Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus, nucleoli and cytoplasm. Recommended concentration of ab122762 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab122762, at 1/1000 dilution, staining HEATR3 in paraffin-embedded Human bone marrow tissue by Immunohistochemistry.
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