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Rabbit Polyclonal HEATR3 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HEATR3 aa 550 to C-terminus.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-HEATR3 antibody (AB122762), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEATR3 antibody (AB122762), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human HEATR3 aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q7Z4Q2

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IF
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/1000.00000 - 1/2500.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1.00000-4.00000 µg/mL

Notes

Recommend PFA Fixation and Triton X-100 treatment

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Plays a role in ribosome biogenesis and in nuclear import of the 60S ribosomal protein L5/large ribosomal subunit protein uL18 (RPL5) (PubMed:35213692). Required for proper erythrocyte maturation (PubMed:35213692).

Alternative names

Recommended products

Rabbit Polyclonal HEATR3 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HEATR3 aa 550 to C-terminus.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human HEATR3 aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q7Z4Q2
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

HEATR3 also known as HEAT repeat-containing protein 3 is a protein characterized by HEAT repeats which are tandemly repeated motifs facilitating protein-protein interactions. This protein has a mass of approximately 94 kDa. Researchers have identified its expression in various tissues suggesting its broad role in cellular function. The structural design of HEATR3 implies it might act as a molecular scaffold helping in processes that need complex assemblies.

Biological function summary

HEATR3 assists in translation machinery modulation. It forms part of the R2TP/Prefoldin-like complex a multi-protein complex engaged in the assembly and maturation of large protein complexes. This involvement implies an important role in maintaining the stability and function of essential cellular structures. Additionally HEATR3 interacts with proteins containing HEAT repeats hinting at remarkable versatility in cellular tasks.

Pathways

HEATR3 is important in the process of ribosome biogenesis and cellular stress response pathways. It works closely with the PI3K/AKT signaling pathway aiding in the regulation of growth and survival signals. The interaction with related pathway proteins such as PIH1D1 indicates HEATR3's participation in cellular systems affecting proliferation and apoptosis.

Associated diseases and disorders

HEATR3 expression and function appear linked to cancer and neurodegenerative diseases. Abnormal expression patterns or mutations in HEATR3 might contribute to the pathogenesis of specific cancer types potentially through disrupted interactions with MTOR or other cancer-related proteins. Additionally the protein's role in proper cellular assembly and stress response implicates it in neurodegenerative disorders where these processes are often impaired.

Product promise

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2 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-HEATR3 antibody (ab122762), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-HEATR3 antibody (ab122762)

    Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus, nucleoli and cytoplasm. Recommended concentration of ab122762 1-4 µg/ml. Cells treated with PFA/Triton X-100.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEATR3 antibody (ab122762), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEATR3 antibody (ab122762)

    ab122762, at 1/1000 dilution, staining HEATR3 in paraffin-embedded Human bone marrow tissue by Immunohistochemistry.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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