JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB220780

Anti-HEATR5B antibody

Be the first to review this product! Submit a review

|

(2 Publications )

Rabbit Polyclonal HEATR5B antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HEATR5B aa 700 to C-terminus.

View Alternative Names

KIAA1414, p200, p200a, HEATR5B, HEAT repeat-containing protein 5B

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-HEATR5B antibody (AB220780)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HEATR5B antibody (AB220780)

Immunofluorescent analysis of A431 cells (PFA-fixed/Triton X-100 permeabilized) labeling HEATR5B with ab220780 at 4 μg/mL (green).

Western blot - Anti-HEATR5B antibody (AB220780)
  • WB

Supplier Data

Western blot - Anti-HEATR5B antibody (AB220780)

All lanes:

Western blot - Anti-HEATR5B antibody (ab220780) at 1/100 dilution

Lane 1:

RT4 cell lysate

Lane 2:

U251 MG cell lysate

Predicted band size: 224 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human HEATR5B aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9P2D3

style
left-column

Complementary Products

Primary Antibodies

AB186831

Anti-DP1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DP1 antibody (AB186831)

  • 2
  • 3
Primary Antibodies

AB231044

Anti-COL16A1 antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL16A1 antibody - C-terminal (AB231044)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HEATR5B also known as CG11316-PB is a protein composed of approximately 205 kDa. The HEATR5B protein consists of HEAT repeat motifs which often facilitate protein-protein interactions. This protein expresses in various human tissues including high levels in the brain and liver. The presence of HEAT repeats suggests that HEATR5B might function as a scaffold organizing multiprotein complexes for specific cellular processes due to their architectural role in assembling proteins.
Biological function summary

HEATR5B participates actively in cellular maintenance by being part of larger multiprotein assemblies. The protein interacts with other cellular proteins supporting their stability and proper functioning. Part of its biological roles likely involves regulation due to its structural conformation which can support versatile coordination of signaling or structural complexes. Current research might be exploring its specific role given its wide expression in vital organs.

Pathways

Many cellular mechanisms rely on HEATR5B due to its structural involvement in molecular signaling and transport pathways. It associates with the mTOR signaling pathway which is essential for regulating cell growth and proliferation. Through these interactions HEATR5B might interface with key components such as mTOR and Raptor serving as a conduit that connects various cellular functions to nutrient and energy sensing.

HEATR5B shows potential relevance to neurodegenerative diseases and some forms of cancer. Disturbances in its normal expression or function could relate to disrupted cellular signaling pathways observed in brain disorders such as Alzheimer's disease. Additionally its association with proteins involved in cell growth regulation suggests a possible link to cancerous processes. Further investigation is essential to verify the direct connections and interactions including how it interacts with associated proteins such as Beta-amyloid in these disorders.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Component of clathrin-coated vesicles (PubMed : 15758025). Component of the aftiphilin/p200/gamma-synergin complex, which plays roles in AP1G1/AP-1-mediated protein trafficking including the trafficking of transferrin from early to recycling endosomes, and the membrane trafficking of furin and the lysosomal enzyme cathepsin D between the trans-Golgi network (TGN) and endosomes (PubMed : 15758025).
See full target information HEATR5B
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 20:3956-3971 PubMed39113702

2024

PKCα Induced the Generation of Extracellular Vesicles in Activated Platelets to Promote Breast Cancer Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Jinghua Zhao,Huan Tian,Xiaona Zhao,Lan Lan,Huanhuan Liu,Yi Sun,Fengyan Yu

Cancer biomarkers : section A of Disease markers 28:91-100 PubMed32176628

2020

The interactome of the prostate-specific protein Anoctamin 7.

Applications

Unspecified application

Species

Unspecified reactive species

Elina Kaikkonen,Aliisa Takala,Juha-Pekka Pursiheimo,Gudrun Wahlström,Johanna Schleutker
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com