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AB122131

Anti-HEATR6 antibody

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(1 Publication)

Rabbit Polyclonal HEATR6 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human HEATR6 aa 1050 to C-terminus.

View Alternative Names

ABC1, HEATR6, HEAT repeat-containing protein 6, Amplified in breast cancer protein 1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-HEATR6 antibody (AB122131)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HEATR6 antibody (AB122131)

Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli, nuclear membrane, cytoplasm and golgi apparatus. Recommended concentration of ab122131 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEATR6 antibody (AB122131)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEATR6 antibody (AB122131)

ab122131, at a 1/200 dilution, staining HEATR6 in paraffin embedded Human kidney tissue by Immunohistochemistry.

Western blot - Anti-HEATR6 antibody (AB122131)
  • WB

Unknown

Western blot - Anti-HEATR6 antibody (AB122131)

All lanes:

Western blot - Anti-HEATR6 antibody (ab122131) at 1/250 dilution

Lane 1:

RT-4 cell lysate

Lane 2:

U-251 MG cell lysate

Lane 3:

Human Plasma

Lane 4:

Human Liver lysate

Lane 5:

Human Tonsil lysate

Predicted band size: 129 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human HEATR6 aa 1050 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q6AI08

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "<p>Recommend PFA Fixation and Triton X-100 treatment</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HEATR6 also known as "HEAT repeat containing protein 6" is a protein with a molecular mass of approximately 120 kDa. It has several HEAT repeat sequences which are recognized for mediating assembly and transport processes within cells. The expression of HEATR6 is found in multiple tissues with higher levels observed in the lungs liver and kidneys. These characteristics suggest it plays a role in diverse cellular functions leveraging its repeat sequences for interactions with other cellular components.
Biological function summary

HEATR6 contributes to cellular and molecular processes by acting as a scaffold protein. This role involves supporting the formation of multi-protein complexes essential for maintaining cellular structure and function. HEATR6 takes part in assembling ribonucleoprotein particles important for RNA processes. By aiding in the organization of these complexes HEATR6 ensures cellular homeostasis and efficient genetic information flow.

Pathways

HEATR6 interacts within the RNA splicing and transport pathways. Its role in these pathways involves coordination with other proteins like SF3B1 and U2AF2 which participate in pre-mRNA splicing. By facilitating RNA processing through these interactions HEATR6 ensures that pre-mRNA is correctly modified and transported a critical step for gene expression. This alignment with important pathways highlights HEATR6's significance in genetic and molecular fidelity.

Abnormalities in HEATR6 expression or function have associations with certain cancers including lung cancer. Experimental evidence indicates that its interaction with other proteins like HSP90 plays a part in cancer cell proliferation. Furthermore dysfunctional HEATR6 might link to neurodegenerative disorders where protein misfolding occurs. The involvement with key proteins and pathologies highlights the potential of HEATR6 as a clinical marker or therapeutic target in specific diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Amplification-dependent oncogene.
See full target information HEATR6
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Aging 15:10389-10406 PubMed37801482

2023

Novel diagnostic biomarkers of oxidative stress, immunological characterization and experimental validation in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Di Hu,Xiaocong Mo,Luo Jihang,Cheng Huang,Hesong Xie,Ling Jin
View all publications
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Product promise

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