Anti-heavy chain Myosin antibody

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain Myosin antibody (AB124205)

IHC image of Anti-heavy chain Myosin antibody staining in a section of formalin-fixed paraffin-embedded mouse skeletal muscle performed on a Leica BOND^TM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab124205, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• WB

Lab

Western blot - Anti-heavy chain Myosin antibody (AB124205)

All lanes:

Western blot - Anti-heavy chain Myosin antibody (ab124205) at 1 µg/mL

Lane 1:

Skeletal Muscle (Human) Tissue Lysate - fetal normal tissue at 10 µg

Lane 2:

Human heart tissue lysate - total protein (<a href='/en-us/products/unavailable/human-heart-tissue-lysate-total-protein-ab29431'>ab29431</a>) at 10 µg

Lane 3:

Human skeletal muscle tissue lysate - total protein (<a href='/en-us/products/unavailable/human-skeletal-muscle-tissue-lysate-total-protein-ab29330'>ab29330</a>) at 10 µg

Lane 4:

Human small intestine tissue lysate - total protein (<a href='/en-us/products/unavailable/human-small-intestine-tissue-lysate-total-protein-ab29276'>ab29276</a>) at 10 µg

Lane 5:

Heart (Human) Tissue Lysate - fetal normal tissue at 10 µg

Lane 6:

Human skin tissue lysate - total protein (<a href='/en-us/products/unavailable/human-skin-tissue-lysate-total-protein-ab30166'>ab30166</a>) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 224 kDa

Observed band size: 190 kDa,223 kDa,40 kDa,50 kDa

true

Exposure time: 30s

• WB

Unknown

Western blot - Anti-heavy chain Myosin antibody (AB124205)

All lanes:

Western blot - Anti-heavy chain Myosin antibody (ab124205) at 1 µg/mL

Lane 1:

Human skeletal muscle tissue lysate - total protein (<a href='/en-us/products/unavailable/human-skeletal-muscle-tissue-lysate-total-protein-ab29330'>ab29330</a>) at 10 µg

Lane 2:

Skeletal Muscle (Mouse) Tissue Lysate at 10 µg

Lane 3:

Skeletal Muscle (Rat) Tissue Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 224 kDa

Observed band size: 223 kDa,40 kDa

true

Exposure time: 30s