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Mouse Recombinant Monoclonal MYH3 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain Myosin antibody [F1.652] - BSA and Azide free (AB264039), expandable thumbnail
  • Western blot - Anti-heavy chain Myosin antibody [F1.652] - BSA and Azide free (AB264039), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain Myosin antibody [F1.652] - BSA and Azide free (AB264039), expandable thumbnail
  • Western blot - Anti-heavy chain Myosin antibody [F1.652] - BSA and Azide free (AB264039), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-FrIHC-P
Human
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended
Tested
Rat
Tested
Not recommended
Not recommended
Tested

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Target data

Function

Muscle contraction.

Alternative names

Myosin-3, Muscle embryonic myosin heavy chain, Myosin heavy chain 3, SMHCE, MYH3

Recommended products

Mouse Recombinant Monoclonal MYH3 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat samples.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
F1.652
Purification technique
Affinity purification Protein A
Specificity

Cross-reactivity with this protein has not been confirmed experimentally.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab264039 is the carrier-free version of Anti-heavy chain Myosin antibody [F1.652] ab264038.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Heavy chain Myosin also known as MYH3 is a protein involved in muscle contraction. Alternative names for this protein include Myosin heavy chain and MyHC with a molecular mass of approximately 223 kDa. This protein is primarily expressed in skeletal muscle tissues during fetal development. The heavy chain myosin is a part of the larger family of myosin proteins which are motor proteins found in most eukaryotic cells.

Biological function summary

Heavy chain Myosin functions to convert chemical energy from ATP into mechanical energy facilitating muscle contraction. It serves as the backbone for thick filaments playing a significant role in muscle fiber composition. Myosin heavy chain forms a complex with actin filaments and this actin-myosin interaction is essential for muscle contraction and force generation. This protein can associate with different myosin light chains and regulatory proteins which modulate its activity.

Pathways

Heavy chain Myosin is an important player in the muscle contraction pathway. This pathway is critical for muscle development and maintenance influencing how muscles respond to stimuli. Actin and tropomyosin are vital collaborators in this process with the interaction between actin and MyHC being central to contraction. The troponin complex acts as a regulatory unit within the pathway controlling the exposure of active sites on actin filaments.

Associated diseases and disorders

Mutations in the MYH3 gene can cause conditions like Freeman-Sheldon syndrome and distal arthrogryposis type 2A. These disorders are characterized by joint contractures and muscle abnormalities. MYH3 mutations alter the normal function of the myosin protein affecting muscle development. Additionally heavy chain Myosin interacts with other muscle-related proteins such as actin and myosin-binding protein C linking it to various skeletal muscle disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain Myosin antibody [F1.652] - BSA and Azide free (ab264039), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain Myosin antibody [F1.652] - BSA and Azide free (ab264039)

    Immunohistochemical analysis of paraffin-embedded mouse E14.5 tissue labeling Myosin with Anti-heavy chain Myosin antibody [F1.652] ab264038 at 1/500 dilution (2.22 μg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Positive staining on the skeletal muscle of mouse E14.5. The section was incubated with Anti-heavy chain Myosin antibody [F1.652] ab264038 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-heavy chain Myosin antibody [F1.652] ab264038).

  • Western blot - Anti-heavy chain Myosin antibody [F1.652] - BSA and Azide free (ab264039), expandable thumbnail

    Western blot - Anti-heavy chain Myosin antibody [F1.652] - BSA and Azide free (ab264039)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Negative control: Mouse adult skelatal muscle (PMID:3530499).


    The expression profile observed is consistent with what has been described in the literature (PMID: 3530499, 12899730).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-heavy chain Myosin antibody [F1.652] ab264038).

    All lanes: Western blot - Anti-heavy chain Myosin antibody [F1.652] (Anti-heavy chain Myosin antibody [F1.652] ab264038) at 0.222 µg/mL

    Lane 1: Mouse embryo tissue lysate at 20 µg

    Lane 2: Mouse adult skeletal muscle tissue lysate at 20 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 224 kDa

    Observed band size: 250 kDa

    Exposure time: 26s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain Myosin antibody [F1.652] - BSA and Azide free (ab264039), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain Myosin antibody [F1.652] - BSA and Azide free (ab264039)

    Immunohistochemical analysis of paraffin-embedded rat E14.5 tissue labeling Myosin with Anti-heavy chain Myosin antibody [F1.652] ab264038 at 1/500 dilution (2.22 μg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Positive staining on the skeletal muscle of rat E14.5. The section was incubated with Anti-heavy chain Myosin antibody [F1.652] ab264038 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-heavy chain Myosin antibody [F1.652] ab264038).

  • Western blot - Anti-heavy chain Myosin antibody [F1.652] - BSA and Azide free (ab264039), expandable thumbnail

    Western blot - Anti-heavy chain Myosin antibody [F1.652] - BSA and Azide free (ab264039)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID: 3530499, 12899730).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-heavy chain Myosin antibody [F1.652] ab264038).

    All lanes: Western blot - Anti-heavy chain Myosin antibody [F1.652] (Anti-heavy chain Myosin antibody [F1.652] ab264038) at 0.222 µg/mL

    Lane 1: L6 (rat skeletal muscle myoblast), whole cell lysate at 20 µg

    Lane 2: Mouse E14.5 limb tissue lysate at 20 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 224 kDa

    Observed band size: 250 kDa

    Exposure time: 26s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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