Anti-heavy chain Myosin antibody [F1.652] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain Myosin antibody [F1.652] - BSA and Azide free (AB264039)

Immunohistochemical analysis of paraffin-embedded mouse E14.5 tissue labeling Myosin with ab264038 at 1/500 dilution (2.22 μg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on the skeletal muscle of mouse E14.5. The section was incubated with ab264038 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264038).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain Myosin antibody [F1.652] - BSA and Azide free (AB264039)

Immunohistochemical analysis of paraffin-embedded rat E14.5 tissue labeling Myosin with ab264038 at 1/500 dilution (2.22 μg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on the skeletal muscle of rat E14.5. The section was incubated with ab264038 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264038).

• WB

Lab

Western blot - Anti-heavy chain Myosin antibody [F1.652] - BSA and Azide free (AB264039)

Blocking/Dilution buffer : 5% NFDM/TBST.

Negative control : Mouse adult skelatal muscle (PMID : 3530499).

The expression profile observed is consistent with what has been described in the literature (PMID : 3530499, 12899730).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264038).

All lanes:

Western blot - Anti-heavy chain Myosin antibody [F1.652] (<a href='/en-us/products/primary-antibodies/heavy-chain-myosin-antibody-f1652-ab264038'>ab264038</a>) at 0.222 µg/mL

Lane 1:

Mouse embryo tissue lysate at 20 µg

Lane 2:

Mouse adult skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 224 kDa

Observed band size: 250 kDa

false

Exposure time: 26s

• WB

Lab

Western blot - Anti-heavy chain Myosin antibody [F1.652] - BSA and Azide free (AB264039)

Blocking/Dilution buffer : 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID : 3530499, 12899730).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264038).

All lanes:

Western blot - Anti-heavy chain Myosin antibody [F1.652] (<a href='/en-us/products/primary-antibodies/heavy-chain-myosin-antibody-f1652-ab264038'>ab264038</a>) at 0.222 µg/mL

Lane 1:

L6 (rat skeletal muscle myoblast), whole cell lysate at 20 µg

Lane 2:

Mouse E14.5 limb tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 224 kDa

Observed band size: 250 kDa

false

Exposure time: 26s