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Goat Polyclonal HEC1/HEC antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human NDC80 aa 600 to C-terminus.

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Images

Key facts

Isotype

IgG

Host species

Goat

Storage buffer

pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human NDC80 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O14777
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

5 µg/mL

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

0.50000-2.00000 µg/mL

Notes

-

Target data

Function

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:9315664, PubMed:12351790, PubMed:14654001, PubMed:14699129, PubMed:15062103, PubMed:15235793, PubMed:15239953, PubMed:15548592, PubMed:16732327). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:15548592). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020). Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules (PubMed:25743205, PubMed:23891108).

Alternative names

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Goat Polyclonal HEC1/HEC antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human NDC80 aa 600 to C-terminus.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human NDC80 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O14777
Purification technique

Affinity purification Protein G

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

In the context of cell division HEC1 functions as part of the NDC80 complex a critical ensemble of four proteins that include NUF2 SPC24 and SPC25. This complex is vital for microtubule binding and kinetochore function. HEC1 as a subunit of NDC80 ensures kinetic stability and maintains the structural integrity of the kinetochore during the cell cycle. Its function is necessary for accurate chromosome alignment on the metaphase plate which prevents missegregation and aneuploidy.

Activity summary

HEC1 also known as NDC80 or HEC is an important component of the kinetochore a protein complex importantly involved in chromosome segregation during cell division. It is often referred to as HEC-1 with a molecular mass of approximately 80 kDa. HEC1 is expressed ubiquitously in proliferating cells and it plays an important role in the accurate alignment and segregation of chromosomes during mitosis. The protein is found at centromeres where it is essential for proper attachment of microtubules.

Pathways

HEC1 is actively involved in the spindle assembly checkpoint (SAC) a safety mechanism that prevents anaphase onset until all chromosomes are properly aligned. This pathway is important in regulating cell cycle progression and preventing genomic instability. HEC1 also interacts with proteins like BUB1 and MAD2 which are integral to the SAC pathway. HEC1’s associations highlight its role in maintaining mitotic accuracy and integrity in dividing cells.

Associated diseases and disorders

HEC1 has a significant connection to cancer. Aberrant expression or malfunction of HEC1 contributes to chromosomal instability a hallmark of cancer progression. Its interactions with oncogenes and tumor suppressor proteins like TP53 can exacerbate this instability leading to unregulated cell division and tumor growth. Additionally HEC1's role in the cell cycle and proliferation links it to disorders like aneuploidies where chromosome number errors occur further implicating its importance in maintaining chromosomal balance.

Product promise

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3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEC1/HEC antibody (ab117415), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEC1/HEC antibody (ab117415)

    ab117415, at 5 ug/ml staining HEC1/HEC in Formalin-fixed, Paraffin-embedded Human adrenal tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEC1/HEC antibody (ab117415), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEC1/HEC antibody (ab117415)

    ab117415, at 5 ug/ml staining HEC1/HEC in Formalin-fixed, Paraffin-embedded Human liver tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

  • Western blot - Anti-HEC1/HEC antibody (ab117415), expandable thumbnail

    Western blot - Anti-HEC1/HEC antibody (ab117415)

    All lanes: Western blot - Anti-HEC1/HEC antibody (AB117415) at 0.5 µg/mL

    All lanes: HeLa cell lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 74 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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