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AB247878

Anti-HEC1/HEC antibody [EPR5342] - BSA and Azide free

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Rabbit Recombinant Monoclonal HEC1/HEC antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

HEC, HEC1, KNTC2, NDC80, Kinetochore protein NDC80 homolog, Highly expressed in cancer protein, Kinetochore protein Hec1, Kinetochore-associated protein 2, Retinoblastoma-associated protein HEC, HsHec1

1 Images
Western blot - Anti-HEC1/HEC antibody [EPR5342] - BSA and Azide free (AB247878)
  • WB

Unknown

Western blot - Anti-HEC1/HEC antibody [EPR5342] - BSA and Azide free (AB247878)

This data was developed using ab109496, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HEC1/HEC antibody [EPR5342] (<a href='/en-us/products/primary-antibodies/hec1-hec-antibody-epr5342-ab109496'>ab109496</a>) at 1/10000 dilution

Lane 1:

Jurkat lysate at 10 µg

Lane 2:

HeLa lysate at 10 µg

Lane 3:

NCCIT lysate at 10 µg

Lane 4:

Human spleen lysate at 10 µg

Predicted band size: 74 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5342

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab247878 is the carrier-free version of ab109496.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HEC1 also known as NDC80 or HEC is an important component of the kinetochore a protein complex importantly involved in chromosome segregation during cell division. It is often referred to as HEC-1 with a molecular mass of approximately 80 kDa. HEC1 is expressed ubiquitously in proliferating cells and it plays an important role in the accurate alignment and segregation of chromosomes during mitosis. The protein is found at centromeres where it is essential for proper attachment of microtubules.
Biological function summary

In the context of cell division HEC1 functions as part of the NDC80 complex a critical ensemble of four proteins that include NUF2 SPC24 and SPC25. This complex is vital for microtubule binding and kinetochore function. HEC1 as a subunit of NDC80 ensures kinetic stability and maintains the structural integrity of the kinetochore during the cell cycle. Its function is necessary for accurate chromosome alignment on the metaphase plate which prevents missegregation and aneuploidy.

Pathways

HEC1 is actively involved in the spindle assembly checkpoint (SAC) a safety mechanism that prevents anaphase onset until all chromosomes are properly aligned. This pathway is important in regulating cell cycle progression and preventing genomic instability. HEC1 also interacts with proteins like BUB1 and MAD2 which are integral to the SAC pathway. HEC1’s associations highlight its role in maintaining mitotic accuracy and integrity in dividing cells.

HEC1 has a significant connection to cancer. Aberrant expression or malfunction of HEC1 contributes to chromosomal instability a hallmark of cancer progression. Its interactions with oncogenes and tumor suppressor proteins like TP53 can exacerbate this instability leading to unregulated cell division and tumor growth. Additionally HEC1's role in the cell cycle and proliferation links it to disorders like aneuploidies where chromosome number errors occur further implicating its importance in maintaining chromosomal balance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed : 12351790, PubMed : 14654001, PubMed : 14699129, PubMed : 15062103, PubMed : 15235793, PubMed : 15239953, PubMed : 15548592, PubMed : 16732327, PubMed : 30409912, PubMed : 9315664). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed : 15548592, PubMed : 30409912). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed : 23085020). Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules (PubMed : 23891108, PubMed : 25743205).
See full target information NDC80
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Product promise

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For full details, please see our Terms & Conditions

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