AB101992

Anti-HECTD1 antibody

Name: Anti-HECTD1 antibody (ab101992) | Abcam
Brand: Abcam Limited
SKU: AB101992

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(9 Publications)

Rabbit Polyclonal HECTD1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human HECTD1 aa 1-50.

View Alternative Names

KIAA1131, HECTD1, E3 ubiquitin-protein ligase HECTD1, E3 ligase for inhibin receptor, HECT domain-containing protein 1, EULIR

2 Images

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Immunoprecipitation - Anti-HECTD1 antibody (AB101992)

ab101992 at 1 µg/ml staining HECTD1 in HeLa cell lysate immunoprecipitated using ab101992 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection : Chemiluminescence with exposure time of 3 minutes

All lanes:

Immunoprecipitation - Anti-HECTD1 antibody (ab101992)

Predicted band size: 289 kDa

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Western blot - Anti-HECTD1 antibody (AB101992)

All lanes:

Western blot - Anti-HECTD1 antibody (ab101992) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 289 kDa

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Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human HECTD1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9ULT8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Turkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HECTD1 which stands for HECT Domain E3 Ubiquitin Protein Ligase 1 also known by other names such as KIAA1594 is an important enzyme in the protein degradation process. HECTD1 acts mechanically as an E3 ubiquitin ligase which means it helps attach ubiquitin molecules to specific protein substrates marking them for degradation by the proteasome. The protein typically weighs around 261 kDa and expresses broadly in human tissues including nervous system cardiovascular tissues and epithelial cells indicating its diverse functional roles.

Biological function summary

The enzyme HECTD1 contributes to numerous cellular processes notably protein homeostasis and cellular signaling. It acts as a part of a larger ubiquitination complex interacting with various adaptor proteins to achieve substrate specificity. By regulating the fate of proteins within the cell HECTD1 impacts processes like cell cycle progression apoptosis and stress responses. Studies show that it also plays a role in embryonic development pointing to its function in tissue morphogenesis and organogenesis.

Pathways

HECTD1 has significant roles within the ubiquitin-proteasome pathway and the cell cycle regulatory pathway. It interacts with proteins such as SMAD2 in the TGF-beta signaling pathway which controls cell proliferation and differentiation. This enzyme also coordinates with TRAF6 in immune signaling pathways influencing immune responses. These interactions highlight HECTD1's pivotal role in maintaining cellular function by modulating protein stability and activity.

Mutations or deregulation of HECTD1 associate with neurodevelopmental disorders and cancer. Abnormal HECTD1 activity can disrupt normal brain development linking it to certain neurodevelopmental disorders. In cancer altered expression or function of HECTD1 affects cell proliferation pathways leading to uncontrolled growth. Researchers find HECTD1 interacting with proteins like c-MYC in oncogenic pathways further establishing its relevance in the pathophysiology of human diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PubMed : 33711283). Mediates 'Lys-63'-linked polyubiquitination of HSP90AA1 which leads to its intracellular localization and reduced secretion (By similarity). Negatively regulating HSP90AA1 secretion in cranial mesenchyme cells may impair their emigration and may be essential for the correct development of the cranial neural folds and neural tube closure (By similarity). Catalyzes ubiquitination and degradation of ZNF622, an assembly factor for the ribosomal 60S subunit, in hematopoietic cells, thereby promoting hematopoietic stem cell renewal (PubMed : 33711283).

See full target information HECTD1

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:4985 PubMed38862515

2024

Smooth muscle NF90 deficiency ameliorates diabetic atherosclerotic calcification in male mice via FBXW7-AGER1-AGEs axis.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Xie,Bin Liu,Wen Qiao,Jing-Zhen He,Jie Cheng,Zhao-Yang Wang,Ya-Min Hou,Xu Zhang,Bo-Han Xu,Yun Zhang,Yu-Guo Chen,Ming-Xiang Zhang

The EMBO journal 42:e113328 PubMed37641865

2023

Global histone H2B degradation regulates insulin/IGF signaling-mediated nutrient stress.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiwen Zhu,Dongdong Li,Zeran Jia,Wenhao Zhang,Yuling Chen,Ruixue Zhao,Yan-Ping Zhang,Wen-Hong Zhang,Haiteng Deng,Yinqing Li,Wei Li,Shouhong Guang,Guangshuo Ou

Scientific reports 12:13160 PubMed35915203

2022

The E3 ubiquitin ligase HECTD1 contributes to cell proliferation through an effect on mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Natalie Vaughan,Nico Scholz,Catherine Lindon,Julien D F Licchesi

Journal of immunology research 2022:7375879 PubMed35832649

2022

LMX1B Activated Circular RNA GFRA1 Modulates the Tumorigenic Properties and Immune Escape of Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Min Meng,Yi-Chen Wu

The Journal of biological chemistry 296:100246 PubMed33853758

2021

The deubiquitinase TRABID stabilizes the K29/K48-specific E3 ubiquitin ligase HECTD1.

Applications

Unspecified application

Species

Unspecified reactive species

Lee D Harris,Janic Le Pen,Nico Scholz,Juliusz Mieszczanek,Natalie Vaughan,Simon Davis,Georgina Berridge,Benedikt M Kessler,Mariann Bienz,Julien D F Licchesi

Scientific reports 8:4092 PubMed29511261

2018

Insights into the ubiquitin-proteasome system of human embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Isabel Saez,Seda Koyuncu,Ricardo Gutierrez-Garcia,Christoph Dieterich,David Vilchez

Molecular cell 67:181-193.e5 PubMed28689657

2017

Wnt-Dependent Inactivation of the Groucho/TLE Co-repressor by the HECT E3 Ubiquitin Ligase Hyd/UBR5.

Applications

Unspecified application

Species

Unspecified reactive species

Joshua E Flack,Juliusz Mieszczanek,Nikola Novcic,Mariann Bienz

Chembiochem : a European journal of chemical biolo 18:1415-1427 PubMed28425671

2017

Activity-Based Probes for HECT E3 Ubiquitin Ligases.

Applications

Unspecified application

Species

Unspecified reactive species

Robert Byrne,Thomas Mund,Julien D F Licchesi

Molecular cancer therapeutics 14:952-63 PubMed25646013

2015

Tumor cells chronically treated with a trastuzumab-maytansinoid antibody-drug conjugate develop varied resistance mechanisms but respond to alternate treatments.

Applications

Unspecified application

Species

Unspecified reactive species

Frank Loganzo,Xingzhi Tan,Matthew Sung,Guixian Jin,Jeremy S Myers,Eugene Melamud,Fang Wang,Veronica Diesl,Maximillian T Follettie,Sylvia Musto,My-Hanh Lam,William Hu,Manoj B Charati,Kiran Khandke,Kenny Sung Kyoo Kim,Mike Cinque,Judy Lucas,Edmund Graziani,Andreas Maderna,Christopher J O'Donnell,Kim T Arndt,Hans-Peter Gerber

View all publications

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Product promise

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Anti-HECTD1 antibody

Immunoprecipitation - Anti-HECTD1 antibody (AB101992)

Western blot - Anti-HECTD1 antibody (AB101992)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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Secondary Antibodies

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