AB173572

Anti-HECTD2 antibody [EPR11554]

RabMAb
Recombinant
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(3 Publications)

Rabbit Recombinant Monoclonal HECTD2 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

Probable E3 ubiquitin-protein ligase HECTD2, HECT domain-containing protein 2, HECT-type E3 ubiquitin transferase HECTD2, HECTD2

1 Images

Supplier Data

Western blot - Anti-HECTD2 antibody [EPR11554] (AB173572)

All lanes:

Western blot - Anti-HECTD2 antibody [EPR11554] (ab173572) at 1/1000 dilution

Lane 1:

MCF-7 cell lysates at 10 µg

Lane 2:

NIH:OVCAR-3 cell lysates at 10 µg

Lane 3:

HeLa cell lysates at 10 µg

Predicted band size: 88 kDa

false

Carrier free

Anti-HECTD2 antibody [EPR11554] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11554

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HECTD2 also referred to as HECT domain E3 ubiquitin protein ligase 2 operates as an E3 ubiquitin ligase an important player in the ubiquitination process. Ubiquitination tags proteins for degradation via the proteasome. HECTD2 has an approximate molecular weight of 106 kDa. It’s expressed in various tissues with heightened levels in the brain and testis. The HECT domain in the protein is critical for its ubiquitin transfer function which involves forming a thioester bond with ubiquitin before transferring it to substrate proteins.

Biological function summary

HECTD2 functions in protein homeostasis and regulates cellular stress responses pathways. It associates with other cellular components to form complexes that guide substrate selectivity and ubiquitination. HECTD2’s regulatory role impacts cell cycle progression and apoptosis thereby influencing cell survival. Researchers have observed its interactions with proteins involved in the inflammation process suggesting a role in immune response modulation.

Pathways

HECTD2 acts within the NF-kB signaling pathway and the endoplasmic reticulum-associated degradation (ERAD) pathway. In the NF-kB pathway HECTD2 regulates the degradation of specific inhibitory proteins facilitating the activation of NF-kB a transcription factor important for immune response. It also interacts with other proteins like IκBα. HECTD2's involvement in the ERAD pathway supports its role in ensuring proper folding and degradation of proteins in the endoplasmic reticulum integrating with proteins such as BiP/GRP78.

The function of HECTD2 connects to neurodegenerative diseases and autoimmune disorders. Alterations in HECTD2 function or expression levels have been linked to the pathology of Alzheimer's disease where misfolded proteins accumulate due to inadequate ubiquitin-proteasome pathway activity. Additionally in autoimmune disorders like lupus aberrant HECTD2 activity might modulate the immune response through its interaction with NF-kB pathway components. These associations make HECTD2 a significant focus in disease mechanism research.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.. (Microbial infection) Catalyzes ubiquitination of Botulinum neurotoxin A light chain (LC) of C.botulinum neurotoxin type A (BoNT/A).

See full target information HECTD2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

In vivo (Athens, Greece) 38:1094-1103 PubMed38688591

2024

HECTD2/TNFAIP1 Axis Regulating the p38/JNK Pathway to Promote an Inflammatory Response in Renal Cell Carcinoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Lv,Yongbo Chen,Liangyou Tang,Yuchang Tian,Dong Ren,Nenghong Jian,Taimin Shen

The ISME journal 16:1205-1221 PubMed34972816

2022

Human gut-microbiome-derived propionate coordinates proteasomal degradation via HECTD2 upregulation to target EHMT2 in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tae Young Ryu,Kwangho Kim,Tae-Su Han,Mi-Ok Lee,Jinkwon Lee,Jinhyeon Choi,Kwang Bo Jung,Eun-Jeong Jeong,Da Mi An,Cho-Rok Jung,Jung Hwa Lim,Jaeeun Jung,Kunhyang Park,Moo-Seung Lee,Mi-Young Kim,Soo Jin Oh,Keun Hur,Ryuji Hamamoto,Doo-Sang Park,Dae-Soo Kim,Mi-Young Son,Hyun-Soo Cho

International journal of molecular sciences 20: PubMed31756985

2019

CGRP Signaling via CALCRL Increases Chemotherapy Resistance and Stem Cell Properties in Acute Myeloid Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Tobias Gluexam,Alexander M Grandits,Angela Schlerka,Chi Huu Nguyen,Julia Etzler,Thomas Finkes,Michael Fuchs,Christoph Scheid,Gerwin Heller,Hubert Hackl,Nathalie Harrer,Heinz Sill,Elisabeth Koller,Dagmar Stoiber,Wolfgang Sommergruber,Rotraud Wieser

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HECTD2 antibody [EPR11554]

Western blot - Anti-HECTD2 antibody [EPR11554] (AB173572)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise