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AB241413

Anti-HECTD3 antibody

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(1 Publication)

Rabbit Polyclonal HECTD3 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human E3 ubiquitin-protein ligase HECTD3 aa 800-900.

View Alternative Names

E3 ubiquitin-protein ligase HECTD3, HECT domain-containing protein 3, HECT-type E3 ubiquitin transferase HECTD3, HECTD3

2 Images
Immunoprecipitation - Anti-HECTD3 antibody (AB241413)
  • IP

Supplier Data

Immunoprecipitation - Anti-HECTD3 antibody (AB241413)

HECTD3 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241413 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab241413 at 1 μg/ml.

Lane 1 : ab241413 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 minutes.

Lysates prepared using NETN lysis buffer.

All lanes:

Immunoprecipitation - Anti-HECTD3 antibody (ab241413)

Predicted band size: 97 kDa

false

Western blot - Anti-HECTD3 antibody (AB241413)
  • WB

Supplier Data

Western blot - Anti-HECTD3 antibody (AB241413)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-HECTD3 antibody (ab241413) at 1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 97 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human E3 ubiquitin-protein ligase HECTD3 aa 800-900. The exact immunogen used to generate this antibody is proprietary information.

Q5T447

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Primary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab241413 was affinity purified using an epitope specific to HECTD3 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HECTD3 also known as hectd3 ubiquitin protein ligase acts as an E3 ubiquitin ligase. It plays an important role in tagging proteins for degradation through the ubiquitin-proteasome system. The molecular weight of HECTD3 is approximately 110 kDa. HECTD3 is mainly expressed in the brain and various immune cells indicating its involvement in neurological and immune functions.
Biological function summary

The enzyme facilitates the regulation of proteins by adding ubiquitin molecules which marks them for degradation or signaling. This function helps control the levels of target proteins and maintain cellular homeostasis. HECTD3 often forms part of a protein complex enabling its ligase activity to be moderated by interacting proteins.

Pathways

The enzyme integrates into the ubiquitin-proteasome pathway and the NF-kB signaling pathway. In the ubiquitin-proteasome system HECTD3 collaborates with other E2 and E1 enzymes to achieve efficient ubiquitination. It interacts directly with IκB proteins in the NF-kB signaling pathway impacting inflammatory and immune responses by controlling the stability and activity of these proteins.

HECTD3's malfunctions correlate to various cancers and immune disorders. For example its dysregulation affects certain types of leukemia where it modifies the degradation process of tumor suppressor proteins. Moreover HECTD3 interacts with proteins such as TP53 which also plays a significant role in the development of cancers. Changes in HECTD3 expression in immune cells influence immune disorders connecting it to autoimmune diseases where immune regulation becomes disrupted.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin ligases accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates ubiquitination of TRIOBP and its subsequent proteasomal degradation, thus facilitating cell cycle progression by regulating the turn-over of TRIOBP. Mediates also ubiquitination of STX8 (By similarity).
See full target information E3 ubiquitin-protein ligase HECTD3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 5:564 PubMed35681048

2022

Stabilization of CDK6 by ribosomal protein uS7, a target protein of the natural product fucoxanthinol.

Applications

Unspecified application

Species

Unspecified reactive species

Yosuke Iizumi,Yoshihiro Sowa,Wakana Goi,Yuichi Aono,Motoki Watanabe,Yoichi Kurumida,Tomoshi Kameda,Kenichi Akaji,Masatoshi Kitagawa,Toshiyuki Sakai
View all publications
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Product promise

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