JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB288750

Anti-HEF1/NEDD-9 antibody [2G9]

Be the first to review this product! Submit a review

|

(0 Publication)

Mouse Monoclonal HEF1/NEDD-9 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human NEDD9 aa 50-400.
1 Images
Western blot - Anti-HEF1/NEDD-9 antibody [2G9] (AB288750)
  • WB

Supplier Data

Western blot - Anti-HEF1/NEDD-9 antibody [2G9] (AB288750)

Western blot using ab288750 monoclonal anti-HEF1 antibody (clone 2G9) antibody shows detection of a ~92 kDa band corresponding to HEF1 in MCF7 lysate [arrowhead]. Approximately 35 μg of lysate was loaded for SDS-PAGE followed by transfer onto nitrocellulose and reaction with a 1 : 1,000 dilution of anti-HEF1 antibody. Detection occurred using a 1 : 5,000 dilution of IRDye®800 conjugated Sh-a-Mouse IgG [H&L] for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers (indicated at left). IRDye®800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

All lanes:

Western blot - Anti-HEF1/NEDD-9 antibody [2G9] (ab288750) at 1/1000 dilution

All lanes:

MCF7 lysate

Secondary

All lanes:

IRDye®800 conjugated Sh-a-Mouse IgG [H&L] at 1/5000 dilution

Predicted band size: 92 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2G9

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human NEDD9 aa 50-400. The exact immunogen used to generate this antibody is proprietary information.

Q14511

Specificity

<p>Reactivity occurs against human, mouse and rat forms of the protein. Reactivity against multiple isoforms is expected. Reactivity against homologues from other sources is not known. Specificity was determined by partial epitope mapping.</p>

style
left-column

Complementary Products

Primary Antibodies

AB151555

Anti-Dynamin 2 antibody [EPR9053(2)(ABC)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 2 antibody [EPR9053(2)(ABC)] (AB151555)

  • 4
  • 1
Primary Antibodies

AB125031

Anti-MC1-R antibody [EPR6530]

20ul selling size|RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MC1-R antibody [EPR6530] (AB125031)

  • 4
  • 2
Primary Antibodies

AB81298

Anti-FAK (phospho Y397) antibody [EP2160Y]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-FAK (phospho Y397) antibody [EP2160Y] (AB81298)

  • 4
  • 2
Primary Antibodies

AB40855

Anti-Smad2 antibody [EP784Y]

20ul selling size|RabMAb|Advanced Validation|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad2 antibody [EP784Y] (AB40855)

  • 5
  • 4
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
This Protein A purified antibody is directed against human HEF1 protein. The product was purified from tissue culture supernatant by chromatography.
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HEF1 also known as NEDD9 is an intracellular signaling protein and a member of the Crk-associated substrate family. It has a molecular mass of approximately 93 kDa. The expression of this protein occurs mainly in tissues involved in cell movement and proliferation like lymphoid organs and neural tissues. It is part of the CAS (Crk-associated substrate) proteins hence associated with a wide range of cellular mechanics.
Biological function summary

HEF1 functions as a scaffolding protein involved in various cellular processes including cell adhesion migration and proliferation. It interacts with several other proteins and forms part of complexes such as focal adhesion complexes. It regulates the dynamics of these complexes by modulating signaling pathways linked to cellular adhesion and migration. In neuronal cells it influences axonal and dendritic branching.

Pathways

HEF1 plays a significant role in both the integrin signaling pathway and cell cycle regulation. Through these pathways HEF1 interacts with various key proteins including focal adhesion kinase (FAK) and Src kinase. It positions itself strategically within these pathways and facilitates cross-talk between signaling molecules enabling the coordination of structural and replicative functions in cells.

HEF1 has been implicated in the progression of cancers such as breast cancer and melanoma. Its overexpression often correlates with increased metastatic potential. In breast cancer HEF1 interacts with proteins like Aurora A and BRCA1 linking it to pathways involved in tumor proliferation and invasion. In melanoma alterations in HEF1 levels can affect melanoma cell migration making it a potential target for therapeutic intervention.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Scaffolding protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion (PubMed : 24574519). As a focal adhesion protein, plays a role in embryonic fibroblast migration (By similarity). May play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B- and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRKL and SHPTP2 to the tyrosine phosphorylated form (PubMed : 9020138). Promotes adhesion and migration of lymphocytes; as a result required for the correct migration of lymphocytes to the spleen and other secondary lymphoid organs (PubMed : 17174122). Plays a role in the organization of T-cell F-actin cortical cytoskeleton and the centralization of T-cell receptor microclusters at the immunological synapse (By similarity). Negatively regulates cilia outgrowth in polarized cysts (By similarity). Modulates cilia disassembly via activation of AURKA-mediated phosphorylation of HDAC6 and subsequent deacetylation of alpha-tubulin (PubMed : 17604723). Positively regulates RANKL-induced osteoclastogenesis (By similarity). Required for the maintenance of hippocampal dendritic spines in the dentate gyrus and CA1 regions, thereby involved in spatial learning and memory (By similarity).
See full target information NEDD9

Alternative Names

CASL, NEDD9, Enhancer of filamentation 1, hEF1, CRK-associated substrate-related protein, Cas scaffolding protein family member 2, Neural precursor cell expressed developmentally down-regulated protein 9, Renal carcinoma antigen NY-REN-12, p105, CAS-L, CasL, CASS2, NEDD-9
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com