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AB88584

Anti-HEF1/NEDD-9 antibody

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(2 Publications)

Mouse Polyclonal HEF1/NEDD-9 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NEDD9.

View Alternative Names

CASL, NEDD9, Enhancer of filamentation 1, hEF1, CRK-associated substrate-related protein, Cas scaffolding protein family member 2, Neural precursor cell expressed developmentally down-regulated protein 9, Renal carcinoma antigen NY-REN-12, p105, CAS-L, CasL, CASS2, NEDD-9

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-HEF1/NEDD-9 antibody (AB88584)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HEF1/NEDD-9 antibody (AB88584)

ab88584 at 10μg/ml staining HEF1/NEDD-9 in HeLa cells by Immunofluorescence.

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human NEDD9.

Q14511

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HEF1 also known as NEDD9 is an intracellular signaling protein and a member of the Crk-associated substrate family. It has a molecular mass of approximately 93 kDa. The expression of this protein occurs mainly in tissues involved in cell movement and proliferation like lymphoid organs and neural tissues. It is part of the CAS (Crk-associated substrate) proteins hence associated with a wide range of cellular mechanics.
Biological function summary

HEF1 functions as a scaffolding protein involved in various cellular processes including cell adhesion migration and proliferation. It interacts with several other proteins and forms part of complexes such as focal adhesion complexes. It regulates the dynamics of these complexes by modulating signaling pathways linked to cellular adhesion and migration. In neuronal cells it influences axonal and dendritic branching.

Pathways

HEF1 plays a significant role in both the integrin signaling pathway and cell cycle regulation. Through these pathways HEF1 interacts with various key proteins including focal adhesion kinase (FAK) and Src kinase. It positions itself strategically within these pathways and facilitates cross-talk between signaling molecules enabling the coordination of structural and replicative functions in cells.

HEF1 has been implicated in the progression of cancers such as breast cancer and melanoma. Its overexpression often correlates with increased metastatic potential. In breast cancer HEF1 interacts with proteins like Aurora A and BRCA1 linking it to pathways involved in tumor proliferation and invasion. In melanoma alterations in HEF1 levels can affect melanoma cell migration making it a potential target for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Scaffolding protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion (PubMed : 24574519). As a focal adhesion protein, plays a role in embryonic fibroblast migration (By similarity). May play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B- and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRKL and SHPTP2 to the tyrosine phosphorylated form (PubMed : 9020138). Promotes adhesion and migration of lymphocytes; as a result required for the correct migration of lymphocytes to the spleen and other secondary lymphoid organs (PubMed : 17174122). Plays a role in the organization of T-cell F-actin cortical cytoskeleton and the centralization of T-cell receptor microclusters at the immunological synapse (By similarity). Negatively regulates cilia outgrowth in polarized cysts (By similarity). Modulates cilia disassembly via activation of AURKA-mediated phosphorylation of HDAC6 and subsequent deacetylation of alpha-tubulin (PubMed : 17604723). Positively regulates RANKL-induced osteoclastogenesis (By similarity). Required for the maintenance of hippocampal dendritic spines in the dentate gyrus and CA1 regions, thereby involved in spatial learning and memory (By similarity).
See full target information NEDD9
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer biotherapy & radiopharmaceuticals 34:405-412 PubMed31411930

2019

miR-5195-3p Suppresses Cell Proliferation and Induces Apoptosis by Directly Targeting NEDD9 in Osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Luowen Wang,Genbing Shi,Donghui Zhu,Yongjia Jin,Xuan Yang

Open biology 6: PubMed27655733

2016

Differential sub-nuclear distribution of hypoxia-inducible factors (HIF)-1 and -2 alpha impacts on their stability and mobility.

Applications

Unspecified application

Species

Unspecified reactive species

S E Taylor,J Bagnall,D Mason,R Levy,D G Fernig,V See
View all publications
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Product promise

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