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AB243073

Anti-HEL antibody [5E8]

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(1 Publication)

Mouse Monoclonal HEL antibody. Suitable for WB, Flow Cyt and reacts with Chemical samples. Cited in 1 publication. Immunogen corresponding to Chemical / Small Molecule corresponding to HEL.

View Alternative Names

Hexanoyl Lys

3 Images
Flow Cytometry - Anti-HEL antibody [5E8] (AB243073)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-HEL antibody [5E8] (AB243073)

Flow cytometric analysis of 90% methanol-fixed SH-SY5Y (human neuroblastoma cell line from bone marrow) cells labeling HEL with ab243073 at 1/50 dilution for 30 mins on ice (solid line) compared with an Isotype control IgG (dashed line). A Goat Anti-Mouse : PE at 1/100 dilution was used as the secondary antibody (20 mins at RT).

Western blot - Anti-HEL antibody [5E8] (AB243073)
  • WB

Supplier Data

Western blot - Anti-HEL antibody [5E8] (AB243073)

Primary incubation for 2 hrs at RT.

Secondary incubation for 1 hr at RT.

Block : 5% Skimmed milk in TBST.

All lanes:

Western blot - Anti-HEL antibody [5E8] (ab243073) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 12 µg

Lane 2:

HeLa (treated with hydrogen peroxide) cell lysate at 12 µg

Secondary

All lanes:

Goat Anti-Mouse IgG: HRP at 1/2000 dilution

true

Exposure time: 5min

Western blot - Anti-HEL antibody [5E8] (AB243073)
  • WB

Supplier Data

Western blot - Anti-HEL antibody [5E8] (AB243073)

Primary incubation for 2 hours at room temperature.

Secondary incubation for 1 hour at room temperature.

Block : 5% Skimmed milk in TBST.

All lanes:

Western blot - Anti-HEL antibody [5E8] (ab243073) at 1/1000 dilution

Lane 1:

Hexanoyl Lysine-BSA at 0.5 µg

Lane 2:

BSA at 0.5 µg

Secondary

All lanes:

Goat Anti-Mouse IgG: HRP at 1/2000 dilution

true

Exposure time: 5min

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5E8

Isotype

IgG1

Carrier free

No

Applications

Flow Cyt, WB

applications

Specificity

Specific for Hexanoyl-Lysine adduct (HEL) modified peptides & proteins. Does not detect free HEL. Does not X-react with Acrolein, Crotonaldehyde, 4-HHE, 4-HNE, MDA, or MGO modified proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from TCS.
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HEL or hen egg white lysozyme is an enzyme that cleaves the glycosidic linkages in the polysaccharides of bacterial cell walls. This enzyme has a known mass of approximately 14 kDa. You can find HEL expressed in various tissues primarily within the egg whites of birds. There are several forms of HEL including HEL full form which refers to its complete structure used in studies assessing enzyme-substrate interactions. Researchers often use anti-HEL antibodies to track HEL activity or in assays like 30 minutes of HEL which can indicate enzyme activity within a specific time frame.
Biological function summary

HEL plays an important role in innate immune defense by degrading peptidoglycan components of bacterial cell membranes. It does not function within a larger protein complex but possesses its antimicrobial activity independently. Typically found in saliva tears and mucus HEL contributes to the first line of defense against bacterial invasion. HEL's antimicrobial property also positions it as an influential protein for studies concerning bacterial resistance and immune response in higher organisms.

Pathways

HEL participates mainly in the defense mechanism pathways including the peptidoglycan degradation pathway. This pathway which involves breaking down bacterial cell walls links HEL intimately with proteins like N-acetylmuramyl-L-alanine amidase. While HEL acts alone in its enzymatic activity it complements actions of other antimicrobial proteins to form a robust defense network. Connections between HEL's role and other pathways may provide insights into broader immune responses but these remain a focused area for study.

HEL is particularly connected to infectious diseases linked with bacterial pathogens such as bacterial conjunctivitis. In such cases HEL activity correlates with proteins involved in local immune responses including lysozyme C. Though HEL's direct connection to non-infectious disorders remains less explored its application in disease models serves as a stepping stone for understanding other disorders. Paying attention to HEL and associated pathways through anti-HEL and anti-lysozyme antibodies can lend more understanding in pathological studies focused on bacterial infections.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information HEL
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39769037

2025

L. Leaf Extract Dose-Dependently Modulates Oxidative Stress in the Kidney and Exerts Anti-Fibrotic and Anti-Inflammatory Properties by the Molecular Mechanisms Independent of NRF-2 Signalization Mirroring the Effects of Losartan in SHR.

Applications

Unspecified application

Species

Unspecified reactive species

Una-Jovana Vajic,Nevena Mihailovic-Stanojevic,Danijela Karanovic,Maja Zivotic,Milan Ivanov,Djurdjica Jovovic,Jelica Grujic-Milanovic,Zoran Miloradovic
View all publications
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