AB202141

Anti-HELB antibody - C-terminal

Name: Anti-HELB antibody - C-terminal (ab202141) | Abcam
Brand: Abcam Limited
SKU: AB202141
Price: 510 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal HELB antibody. C-terminal. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human HELB aa 1000 to C-terminus.

View Alternative Names

DNA helicase B, hDHB, HELB

2 Images

Supplier Data

Immunoprecipitation - Anti-HELB antibody - C-terminal (AB202141)

Lane 1 : Detection of HELB in immunoprecipitates of 293T whole cell lysate (prepared using NETN lysis buffer; 0.5 or 1.0 mg per IP reaction, 20% of IP loaded) using ab202141 at 6 μg per reaction, and at 0.4 μg/ml for subsequent Western blot detection.

Lane 2 : Control IgG
Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-HELB antibody - C-terminal (ab202141)

Predicted band size: 123 kDa

true

Exposure time: 30s

Supplier Data

Western blot - Anti-HELB antibody - C-terminal (AB202141)

All lanes:

Western blot - Anti-HELB antibody - C-terminal (ab202141) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK-1 whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 123 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human HELB aa 1000 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8NG08

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HELB also known as DNA helicase B plays an important role in DNA replication and repair. This protein has a mass of approximately 112 kDa. It acts as a DNA helicase unwinding the duplex DNA during replication and repair. HELB is expressed mainly in the nucleus of proliferating cells particularly in tissues with high cell division rates like the testes and bone marrow. Important for maintaining genome stability HELB interacts with various DNA structures to facilitate repair processes.

Biological function summary

DNA helicase B takes part in the maintenance of genomic integrity through its helicase activity. HELB does not form a known complex but acts synergistically with other proteins during DNA synthesis and repair. It interacts with replication protein A (RPA) enhancing its ability to assist in DNA repair. The protein influences DNA damage response preventing replication fork stalling and promoting cell cycle progression.

Pathways

HELB functions in important DNA repair pathways like homologous recombination and base excision repair. Through these pathways it collaborates with key proteins such as ATM and ATR kinases. HELB regulates the DNA damage response by modulating the action of these kinases which are vital for cell cycle checkpoints and repair mechanisms.

HELB has associations with cancer and genomic instability disorders. Alterations in HELB expression can impact DNA repair proficiency potentially leading to oncogenesis. Mutations or dysfunctions in HELB may contribute to the development of cancer by allowing DNA damage accumulation. Additionally HELB's interaction with proteins such as BRCA1 indicates its involvement in breast cancer where ineffective repair of DNA damage can promote tumorigenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

5'-3' DNA helicase involved in DNA damage response by acting as an inhibitor of DNA end resection (PubMed : 25617833, PubMed : 26774285). Recruitment to single-stranded DNA (ssDNA) following DNA damage leads to inhibit the nucleases catalyzing resection, such as EXO1, BLM and DNA2, possibly via the 5'-3' ssDNA translocase activity of HELB (PubMed : 26774285). As cells approach S phase, DNA end resection is promoted by the nuclear export of HELB following phosphorylation (PubMed : 26774285). Acts independently of TP53BP1 (PubMed : 26774285). Unwinds duplex DNA with 5'-3' polarity. Has single-strand DNA-dependent ATPase and DNA helicase activities. Prefers ATP and dATP as substrates (PubMed : 12181327). During S phase, may facilitate cellular recovery from replication stress (PubMed : 22194613).

See full target information HELB

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

NAR molecular medicine 2:ugaf019 PubMed40496644

2025

Rare SNP in the gene interferes with RPA interaction and cellular function of HELB.

Applications

Unspecified application

Species

Unspecified reactive species

Bertha Osei,Benjamin H May,Joseph S Beard,Matthew D Thompson,Duah Alkam,Maroof Khan Zafar,Erik Bergstrom,Stephanie D Byrum,Eric J Enemark,Kirk L West,Alicia K Byrd

Molecular cell 81:4440-4456.e7 PubMed34597596

2021

The Protexin complex counters resection on stalled forks to promote homologous recombination and crosslink repair.

Applications

Unspecified application

Species

Unspecified reactive species

Richard O Adeyemi,Nicholas A Willis,Andrew E H Elia,Connor Clairmont,Shibo Li,Xiaohua Wu,Alan D D'Andrea,Ralph Scully,Stephen J Elledge

Biochemistry 59:2401-2409 PubMed32478505

2020

The Expression of Human DNA Helicase B Is Affected by G-Quadruplexes in the Promoter.

Applications

Unspecified application

Species

Unspecified reactive species

Maroof Khan Zafar,Lindsey Hazeslip,Muhammad Zain Chauhan,Alicia K Byrd

Journal of molecular histology 51:3-13 PubMed31722080

2019

Natriuretic peptide system expression in murine and human submandibular salivary glands: a study of the spatial localisation of ANB, BNP, CNP and their receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Araz Ahmed,Alessandro Gulino,Simita Amayo,Walter Arancio,Ada Maria Florena,Beatrice Belmonte,Abdo Jurjus,Angelo Leone,Isabelle Miletich

View all publications

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Product promise

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Anti-HELB antibody - C-terminal

Immunoprecipitation - Anti-HELB antibody - C-terminal (AB202141)

Western blot - Anti-HELB antibody - C-terminal (AB202141)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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