AB231433

Anti-Helicobacter pylori antibody [SPM526]

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(2 Publications)

Mouse Monoclonal Helicobacter pylori antibody. Suitable for IHC-P and reacts with Helicobacter pylori samples. Cited in 2 publications.

View Alternative Names

H. pylori

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Helicobacter pylori antibody [SPM526] (AB231433)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Helicobacter pylori antibody [SPM526] (AB231433)

Formalin-fixed, paraffin-embedded human H. pylori-infected stomach tissue stained for Helicobacter pylori using ab231433 at 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

SPM526

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Helicobacter pylori

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Helicobacter pylori": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>Boil tissue section in Citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.</p><p>Incubate with primary antibody for 30 minutes at room temperature.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from TCS by Protein A/G.

Storage buffer

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Helicobacter pylori often called H. pylori is a gram-negative spiral-shaped bacterium with a mass of about 3.39 megadaltons. This organism colonizes the human gastric epithelium where it survives in the acidic environment of the stomach. The bacterium expresses urease which breaks down urea to ammonia and carbon dioxide helping neutralize gastric acid. It attaches to the gastric mucosa using flagella for motility and adheres to host cells via adhesins. Researchers utilize specific staining methods such as the Helicobacter pylori stain and tools like anti-Helicobacter antibodies to detect and study this pathogen.

Biological function summary

Helicobacter pylori plays a significant role in gastric health by disrupting mucosal integrity. It engages in the secretion of virulence factors like CagA and VacA leading to changes in cellular signaling and inflammation. H. pylori does not operate as part of a large complex but its interactions with the gastric mucosa have substantial effects. Detection and diagnosis often employ tools like H. pylori IHC and H. pylori antibody kits which target the bacterium's proteins to confirm infection presence.

Pathways

H. pylori infection activates signaling routes including the NF-κB pathway and the MAPK pathway in gastric epithelial cells. These pathways contribute to inflammatory responses and immune cell activation leading to mucosal damage. Within these pathways proteins like tumor necrosis factor-alpha (TNF-α) and interleukin-8 (IL-8) play roles in mediating inflammation initiated by the bacterial colonization of the gastric lining.

Chronic H. pylori infection has strong associations with gastric ulcers and gastric cancer. The bacterium promotes persistent inflammation and cellular damage that can progress to malignancy. H. pylori-related disorders are linked with proteins such as CagA a known oncogenic factor that interferes with host cell signaling and promotes carcinogenesis. Detection and specific targeting by Helicobacter pylori antibodies play important roles in managing and studying these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Helicobacter pylori

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:669 PubMed38253620

2024

LOX-1 acts as an N-methyladenosine-regulated receptor for Helicobacter pylori by binding to the bacterial catalase.

Applications

Unspecified application

Species

Unspecified reactive species

Judeng Zeng,Chuan Xie,Ziheng Huang,Chi H Cho,Hung Chan,Qing Li,Hassan Ashktorab,Duane T Smoot,Sunny H Wong,Jun Yu,Wei Gong,Cong Liang,Hongzhi Xu,Huarong Chen,Xiaodong Liu,Justin C Y Wu,Margaret Ip,Tony Gin,Lin Zhang,Matthew T V Chan,Wei Hu,William K K Wu

Pathology oncology research : POR 28:1610684 PubMed36561231

2022

Negative correlation between the nuclear size and nuclear Lamina component Lamin A in intraductal papillary mucinous neoplasms of the pancreas.

Applications

Unspecified application

Species

Unspecified reactive species

Tamaki Hiroe,Shunichi Moriya,Sayaka Kobayashi,Yoshimi Nishijima,Akira Watanabe,Ken Shirabe,Hayato Ikota,Hideaki Yokoo,Masanao Saio

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Helicobacter pylori antibody [SPM526]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Helicobacter pylori antibody [SPM526] (AB231433)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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