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AB303693

Anti-Hemagglutinin antibody [EPR24541-73] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal Hemagglutinin antibody. Carrier free. Suitable for sELISA and reacts with Influenza A samples.

View Alternative Names

Hemagglutinin, HA

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Sandwich ELISA - Anti-Hemagglutinin antibody [EPR24541-73] - BSA and Azide free (Detector) (AB303693)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Hemagglutinin antibody [EPR24541-73] - BSA and Azide free (Detector) (AB303693)

Sandwich ELISA of ab303691 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24541-73

Isotype

IgG

Carrier free

Yes

Reacts with

Influenza A

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Influenza A": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Influenza A Virus Hemagglutinin also known as hemagglutinin or influenza HA is a surface glycoprotein found on the influenza A virus. Hemagglutinin has a mass of approximately 75 kDa and serves as an important player in the virus's ability to infect host cells. It is expressed on the exterior membrane of the influenza virus and allows the virus to attach to and enter host cells initiating the infection process. This protein comes in various subtypes adding complexity to the virus's infection strategies and complicating vaccine development.
Biological function summary

Hemagglutinin facilitates the binding of the virus to sialic acid receptors on the surface of host epithelial cells. This binding triggers endocytosis allowing the virus entry into the host cell. Hemagglutinin is part of a complex that works closely with neuraminidase another glycoprotein on the virus surface. The hemagglutinin-neuraminidase complex is essential for the viral life cycle balancing attachment to host cells with the release of new viral particles.

Pathways

Hemagglutinin plays a significant role in the viral infection pathway of the influenza virus. It is critical in the initial steps of the pathway that involve viral entry and fusion with the host cell membrane. Hemagglutinin's interaction with the sialic acid receptors is closely linked with the activity of neuraminidase which aids in the release stage of the infection. This interplay between hemagglutinin and neuraminidase highlights their participation in the viral replication cycle demonstrating their complementary roles.

Hemagglutinin is deeply connected to seasonal influenza outbreaks and pandemics. Alterations in the hemagglutinin protein such as mutations and antigenic drifts or shifts can lead to new influenza strains with pandemic potential. The interaction of hemagglutinin with host immune responses and its capability to change allows the virus to evade immune detection. Neuraminidase inhibitors a class of antiviral drugs target enzymes closely related to hemagglutinin to prevent the spread of infection showcasing hemagglutinin's centrality in both infection and therapeutic intervention strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.
See full target information HA
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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