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Knockout Tested Mouse Monoclonal Heme Oxygenase 1 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human HMOX1 aa 1-50.

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Images

Western blot - Anti-Heme Oxygenase 1 antibody [1F12-A6] (AB223349), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [1F12-A6] (AB223349), expandable thumbnail
  • Immunohistochemistry paraffin embedded sections - Anti-Heme Oxygenase 1 antibody [1F12-A6] (AB223349), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [1F12-A6] (AB223349), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [1F12-A6] (AB223349), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Synthetic Peptide within Human HMOX1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P09601

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-P
Human
Tested
Tested
Expected
Mouse
Expected
Expected
Tested
Cow
Predicted
Predicted
Predicted
Orangutan
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Orangutan, Cow
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Orangutan, Cow
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

Fixation: Bouin's fixative.

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Orangutan, Cow
Dilution info
-
Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Heme oxygenase 1. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron (PubMed:11121422, PubMed:19556236, PubMed:7703255). Affords protection against programmed cell death and this cytoprotective effect relies on its ability to catabolize free heme and prevent it from sensitizing cells to undergo apoptosis (PubMed:20055707). Heme oxygenase 1. (Microbial infection) During SARS-COV-2 infection, promotes SARS-CoV-2 ORF3A-mediated autophagy but is unlikely to be required for ORF3A-mediated induction of reticulophagy. Heme oxygenase 1 soluble form. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron.

Alternative names

HO, HO1, HMOX1, Heme oxygenase 1, HO-1

Recommended products

Knockout Tested Mouse Monoclonal Heme Oxygenase 1 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human HMOX1 aa 1-50.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Synthetic Peptide within Human HMOX1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P09601
Clone number
1F12-A6
Purification technique
Affinity purification Protein G
Specificity

Does not cross-react with Heme Oxygenase 2.

Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Heme Oxygenase 1 also known as HO-1 or HMOX1 is an enzyme that plays an important mechanistic role in heme catabolism. It catalyzes the degradation of heme into biliverdin carbon monoxide and free iron. This process involves the cleavage of the heme ring. HO-1 has a molecular weight of approximately 32 kDa. It is widely expressed in numerous tissues but is especially abundant in the liver and spleen. Its expression is induced by heme and other stress stimuli such as heavy metals cytokines and reactive oxygen species.

Biological function summary

Heme Oxygenase 1 serves important protective functions in the body. It is not part of a larger complex but its products such as carbon monoxide and biliverdin have their own biological activities. Carbon monoxide produced by HO-1 has antiflammatory properties and can modulate apoptotic pathways. Biliverdin is reduced to bilirubin which acts as an antioxidant. The enzyme therefore directly influences cellular stress responses and maintains cellular homeostasis through these processes.

Pathways

Heme Oxygenase 1 is integrally involved in oxidative stress response and heme metabolism. It participates in the cellular response to oxidative damage by reducing oxidative stress and promoting cytoprotection. Through its heme degradation activity it is connected with the synthesis of biologically active molecules like bilirubin and carbon monoxide. Heme Oxygenase 1 activity is related to other proteins in oxidative stress pathways such as Nuclear Factor Erythroid 2-Related Factor 2 (Nrf2) which regulates its expression and globins which are sources of heme for HO-1 activity.

Associated diseases and disorders

Heme Oxygenase 1 has been linked to conditions like cardiovascular diseases and neurodegenerative disorders. Its expression can attenuate the severity of atherosclerosis where oxidative stress is an important factor. In neurodegenerative diseases HO-1’s antioxidant properties may provide neuroprotection by mitigating oxidative damage. The protein's interactions with inflammatory cytokines such as Interleukin-6 and tumor necrosis factor-alpha influence its activity in these disease contexts.

Product promise

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Full details and terms and conditions can be found here:
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6 product images

  • Western blot - Anti-Heme Oxygenase 1 antibody [1F12-A6] (ab223349), expandable thumbnail

    Western blot - Anti-Heme Oxygenase 1 antibody [1F12-A6] (ab223349)

    Blocking: 1.5% BSA for 30 minutes at RT.

    All lanes: Western blot - Anti-Heme Oxygenase 1 antibody [1F12-A6] (ab223349) at 1/1000 dilution

    All lanes: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 15 µg

    Secondary

    All lanes: Sheep Anti-Mouse IgG: HRP

    Predicted band size: 33 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [1F12-A6] (ab223349), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [1F12-A6] (ab223349)

    2% formaldehyde fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Heme Oxygenase 1 (green) using ab223349 at a 1/100 dilution in ICC/IF. Secondary: FITC Goat Anti-Mouse at 1/200 dilution for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 dilution for 2 hours at RT.

    (A) DAPI nuclear stain (B) ab223349 (C) Merge.

  • Immunohistochemistry paraffin embedded sections - Anti-Heme Oxygenase 1 antibody [1F12-A6] (ab223349), expandable thumbnail

    Immunohistochemistry paraffin embedded sections - Anti-Heme Oxygenase 1 antibody [1F12-A6] (ab223349)

    Bouin's fixative, paraffin embedded mouse back skin tissue stained for Heme Oxygenase 1 (green) with ab223349 at a 1/100 dilution in immunohistochemical analysis. Secondary Antibody: FITC Goat Anti-Mouse at 1/50 for 1 hour at RT.

  • Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [1F12-A6] (ab223349), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [1F12-A6] (ab223349)

    2% formaldehyde fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Heme Oxygenase 1 (yellow) using ab223349 at a 1/100 dilution in ICC/IF. Secondary: R-PE Goat Anti-Mouse at 1/200 dilution for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 dilution for 2 hours at RT.

    (A) DAPI nuclear stain (B) ab223349 (C) Merge.

  • Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [1F12-A6] (ab223349), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [1F12-A6] (ab223349)

    100% methanol fixed HaCaT (human keratinocyte cell line) cells stained for Heme Oxygenase 1 (green) using ab223349 at a 1/100 dilution in ICC/IF. Secondary: FITC Goat Anti-Mouse at 1/50 dilution for 1 hour at RT.

  • Western blot - Anti-Heme Oxygenase 1 antibody [1F12-A6] (ab223349), expandable thumbnail

    Western blot - Anti-Heme Oxygenase 1 antibody [1F12-A6] (ab223349)

    False colour image of Western blot: Anti-Heme Oxygenase 1 antibody [1F12-A6] staining at 1/1000 dilution, shown in green; Rabbit anti-alpha Tubulin antibody [EP1332Y] (Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab52866) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab223349 was shown to bind specifically to Heme Oxygenase 1. A band was observed at 33 kDa in wild-type A549 cell lysates with no signal observed at this size in HMOX1 knockout cell line Human HMOX1 (Heme Oxygenase 1) knockout A549 cell line ab269503 (HMOX knockout A549 lysate ab259782). To generate this image, wild-type and HMOX1 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution

    All lanes: Western blot - Anti-Heme Oxygenase 1 antibody [1F12-A6] (ab223349) at 1/1000 dilution

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 2: HMOX1 knockout A549 cell lysate at 20 µg

    Lane 3: Mouse Spleen cell lysate at 20 µg

    Lane 4: HeLa cell lysate at 20 µg

    Lane 5: HEK-293 cell lysate at 20 µg

    Secondary

    All lanes: Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 33 kDa

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