Anti-Heme Oxygenase 1 antibody [23/Heme Oxygenase 1] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [23/Heme Oxygenase 1] - BSA and Azide free (AB302672)

This data was developed using ab302671, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Heme oxygenase 1 with ab302671 at 1/2000 (0.501 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on Kuffer cells of human liver.The section was incubated with ab302671 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [23/Heme Oxygenase 1] - BSA and Azide free (AB302672)

This data was developed using ab302671, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Heme oxygenase 1 with ab302671 at 1/2000 (0.501 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on macrophages of human lung.The section was incubated with ab302671 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-Heme Oxygenase 1 antibody [23/Heme Oxygenase 1] - BSA and Azide free (AB302672)

This data was developed using ab302671, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 8247003 ). The identity of the band(s) between 75kDa~100kDa are unknown. Lanes 3/4/5 in blot was developed using a high sensitivity ECL substrate. Exposure time : Lanes 1-4 : 3minutes

All lanes:

Western blot - Anti-Heme Oxygenase 1 antibody [23/Heme Oxygenase 1] (<a href='/en-us/products/primary-antibodies/heme-oxygenase-1-antibody-23-heme-oxygenase-1-ab302671'>ab302671</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 10 µg

Lane 2:

A549 (human lung carcinoma epithelial cell), whole cell lysate 10 µg

Lane 3:

Untreated THP-1 (human monocytic leukemia monocyte), whole cell lysate 10 µg

Lane 4:

THP-1 treated with 80nm TPA (<a href='/en-us/products/biochemicals/phorbol-12-myristate-13-acetate-pma-pkc-activator-ab120297'>ab120297</a>) for 24hours, then /ml LPS for 30 minutes, whole cell lysate 10 µg

Lane 5:

HT-1080 (human fibrosarcoma epithelial cell), whole cell lysate 10 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 32 kDa

false

Exposure time: 3min