Rabbit Polyclonal Heme Oxygenase 1 antibody. Suitable for IHC-P, WB, sELISA, ICC/IF and reacts with Rat, Mouse, Human samples. Cited in 269 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Hmox1.
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- Nature metabolism 5:2111-21302023Iron accumulation drives fibrosis, senescence and the senescence-associated secretory phenotype.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMate Maus,Vanessa López-Polo,Lidia Mateo,Miguel Lafarga,Mònica Aguilera,Eugenia De Lama,Kathleen Meyer,Anna Sola,Cecilia Lopez-Martinez,Ines López-Alonso,Marc Guasch-Piqueras,Fernanda Hernandez-Gonzalez,Selim Chaib,Miguel Rovira,Mayka Sanchez,Rosa Faner,Alvar Agusti,Rodrigo Diéguez-Hurtado,Sagrario Ortega,Anna Manonelles,Stefan Engelhardt,Freddy Monteiro,Camille Stephan-Otto Attolini,Neus Prats,Guillermo Albaiceta,Josep M Cruzado,Manuel SerranoPubMed 38097808
- Military Medical Research 10:532023Caveolin-1 is critical for hepatic iron storage capacity in the development of nonalcoholic fatty liver disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuang-Hui Deng,Chao-Feng Wu,Yun-Jia Li,Hao Shi,Wei-Chao Zhong,Mu-Keng Hong,Jun-Jie Li,Jia-Min Zhao,Chang Liu,Meng-Chen Qin,Zhi-Yun Zeng,Wei-Min Zhang,Ken Kin Lam Yung,Zhi-Ping Lv,Lei GaoPubMed 37941054
- Glia 72:411-4322023Wnt5a/Ror2 promotes Nrf2-mediated tissue protective function of astrocytes after brain injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMitsuharu Endo,Yuki Tanaka,Mayo Fukuoka,Hayata Suzuki,Yasuhiro MinamiPubMed 37904612
- Scientific reports 13:182392023The lipolysis inhibitor acipimox reverses the cardiac phenotype induced by electronic cigarettes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJorge Espinoza-Derout,Jose Mari Luis Arambulo,William Ramirez-Trillo,Juan Carlos Rivera,Kamrul M Hasan,Candice J Lao,Maria C Jordan,Xuesi M Shao,Kenneth P Roos,Amiya P Sinha-Hikim,Theodore C FriedmanPubMed 37880325
- International journal of molecular sciences 24:2023Flavonoid-Rich Berry Extract Enhances Nrf2/HO-1 Signaling Pathway Activation and Exerts Antiulcerative Effects In Vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBetul Cicek,Betul Danısman,Serkan Yildirim,Neslihan Yuce,Dragana Nikitovic,Ismail Bolat,Mehmet Kuzucu,Ertuğrul Ceyran,Ebru Bardas,Kirill S Golokhvast,Aristidis Tsatsakis,Ali TaghizadehghalehjoughiPubMed 37895164
- Veterinary research 54:782023MicroRNA miR-223 modulates NLRP3 and Keap1, mitigating lipopolysaccharide-induced inflammation and oxidative stress in bovine mammary epithelial cells and murine mammary glands.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMan Zhou,Herman W Barkema,Jian Gao,Jingyue Yang,Yue Wang,John P Kastelic,Sohrab Khan,Gang Liu,Bo HanPubMed 37710276
- Frontiers in medicine 10:12309412023Restored retinal physiology after administration of niacin with citicoline in a mouse model of hypertensive glaucoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlberto Melecchi,Rosario Amato,Massimo Dal Monte,Dario Rusciano,Paola Bagnoli,Maurizio CammalleriPubMed 37731716
- BMC cardiovascular disorders 23:4102023Allicin protects against LPS-induced cardiomyocyte injury by activating Nrf2-HO-1 and inhibiting NLRP3 pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFangyuan Sun,Kailiang Xu,Jiayi Zhou,Wei Zhang,Guihe Duan,Ming LeiPubMed 37596540
- Open medicine (Warsaw, Poland) 18:202307292023miR-141-3p accelerates ovarian cancer progression and promotes M2-like macrophage polarization by targeting the Keap1-Nrf2 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingyun Zhao,Leilei Liu,Wei Zhao,Cuiting Lv,Na Zhang,Xinzhuan Jia,Zhengmao ZhangPubMed 37333452
- International journal of molecular sciences 24:20235-ALA/SFC Ameliorates Endotoxin-Induced Ocular Inflammation in Rats by Inhibiting the NF-κB Signaling Pathway and Activating the HO-1/Nrf2 Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuya Otaka,Kazutaka Kanai,Arisa Mori,Daiki Okada,Noriaki Nagai,Yohei Yamashita,Yoichiro Ichikawa,Kazuki TajimaPubMed 37239995
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Rat Hmox1. The exact immunogen used to generate this antibody is proprietary information. Database link P06762
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Reactivity data
Select an application| IHC-P | WB | sELISA | ICC/IF | |
|---|---|---|---|---|
| Human | Expected | Tested | Expected | Tested |
| Mouse | Expected | Tested | Expected | Expected |
| Rat | Tested | Expected | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1-5 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/2000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes For sandwich ELISA, use this antibody as Detection at 0.5 μg/ml with Mouse monoclonal [HO-1-1] to Heme Oxygenase 1 (Anti-Heme Oxygenase 1 antibody [HO-1-1] ab13248) as Capture. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Heme oxygenase 1. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron (PubMed:11121422, PubMed:19556236, PubMed:7703255). Affords protection against programmed cell death and this cytoprotective effect relies on its ability to catabolize free heme and prevent it from sensitizing cells to undergo apoptosis (PubMed:20055707). Heme oxygenase 1. (Microbial infection) During SARS-COV-2 infection, promotes SARS-CoV-2 ORF3A-mediated autophagy but is unlikely to be required for ORF3A-mediated induction of reticulophagy. Heme oxygenase 1 soluble form. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron.
Alternative names
HO, HO1, HMOX1, Heme oxygenase 1, HO-1
Recommended products
Rabbit Polyclonal Heme Oxygenase 1 antibody. Suitable for IHC-P, WB, sELISA, ICC/IF and reacts with Rat, Mouse, Human samples. Cited in 269 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Hmox1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Rat Hmox1. The exact immunogen used to generate this antibody is proprietary information. Database link P06762
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Heme Oxygenase 1 also known as HO-1 or HMOX1 is an enzyme that plays an important mechanistic role in heme catabolism. It catalyzes the degradation of heme into biliverdin carbon monoxide and free iron. This process involves the cleavage of the heme ring. HO-1 has a molecular weight of approximately 32 kDa. It is widely expressed in numerous tissues but is especially abundant in the liver and spleen. Its expression is induced by heme and other stress stimuli such as heavy metals cytokines and reactive oxygen species.
- Biological function summary
Heme Oxygenase 1 serves important protective functions in the body. It is not part of a larger complex but its products such as carbon monoxide and biliverdin have their own biological activities. Carbon monoxide produced by HO-1 has antiflammatory properties and can modulate apoptotic pathways. Biliverdin is reduced to bilirubin which acts as an antioxidant. The enzyme therefore directly influences cellular stress responses and maintains cellular homeostasis through these processes.
- Pathways
Heme Oxygenase 1 is integrally involved in oxidative stress response and heme metabolism. It participates in the cellular response to oxidative damage by reducing oxidative stress and promoting cytoprotection. Through its heme degradation activity it is connected with the synthesis of biologically active molecules like bilirubin and carbon monoxide. Heme Oxygenase 1 activity is related to other proteins in oxidative stress pathways such as Nuclear Factor Erythroid 2-Related Factor 2 (Nrf2) which regulates its expression and globins which are sources of heme for HO-1 activity.
- Associated diseases and disorders
Heme Oxygenase 1 has been linked to conditions like cardiovascular diseases and neurodegenerative disorders. Its expression can attenuate the severity of atherosclerosis where oxidative stress is an important factor. In neurodegenerative diseases HO-1’s antioxidant properties may provide neuroprotection by mitigating oxidative damage. The protein's interactions with inflammatory cytokines such as Interleukin-6 and tumor necrosis factor-alpha influence its activity in these disease contexts.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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7 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody (ab13243)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody (ab13243)
Western blot - Anti-Heme Oxygenase 1 antibody (ab13243)
Hek293 & HL60 presumed negative or very low expression.
Loading control GAPDH at 38kDa
All lanes: Western blot - Anti-Heme Oxygenase 1 antibody (ab13243) at 1/1000 dilution
Lane 1: Hek293 at 10 µg
Lane 2: HL60 at 10 µg
Lane 3: HeLa at 10 µg
Lane 4: A549 at 10 µg
Lane 5: Hu spleen at 10 µg
Lane 6: Ms spleen at 10 µg
Lane 7: Rt spleen at 10 µg
SecondaryAll lanes: IRDye® 800CW Goat anti Rabbit
Predicted band size: 33 kDa
Observed band size: 32 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody (ab13243)
IHC image of Heme Oxygenase 1 staining in formalin fixed, paraffin embedded rat spleen normal tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13243, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Western blot - Anti-Heme Oxygenase 1 antibody (ab13243)
All lanes: Western blot - Anti-Heme Oxygenase 1 antibody (ab13243)
Predicted band size: 33 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody (ab13243)
Alzheimer diseased section stained with ab13243.
Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody (ab13243)
ICC/IF image of ab13243 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13243, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Sandwich ELISA - Anti-Heme Oxygenase 1 antibody (ab13243)
Standard Curve for Heme Oxygenase 1 (Analyte: Heme Oxygenase 1 protein (Tagged) (Recombinant Human Heme Oxygenase 1 protein ab85243));
dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal [HO-1-1] to Heme
Oxygenase 1 (Anti-Heme Oxygenase 1 antibody [HO-1-1] ab13248) at 5μg/ml and Detector Antibody Rabbit polyclonal to Heme Oxygenase 1
(ab13243) at 0.5μg/ml.
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