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Rabbit Polyclonal Heme Oxygenase 1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Rat, Human, Mouse samples. Cited in 27 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HMOX1 aa 1 to C-terminus.


Images

Western blot - Anti-Heme Oxygenase 1 antibody (AB137749), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody (AB137749), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody (AB137749), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody (AB137749), expandable thumbnail
  • Western blot - Anti-Heme Oxygenase 1 antibody (AB137749), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human HMOX1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P09601

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Rat
Tested
Tested
Expected

Tested
Tested

Species

Rat

Dilution info

1/100 - 1/1000

Notes

Alternatively antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) may be used.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/100 - 1/1000

Notes

Alternatively antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) may be used.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500 - 1/3000

Notes

-

Species

Human

Dilution info

1/500 - 1/3000

Notes

-

Species

Rat

Dilution info

1/500 - 1/3000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/1000

Notes

-

Expected
Expected

Species

Rat, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Heme oxygenase 1Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron (PubMed:11121422, PubMed:19556236, PubMed:7703255). Affords protection against programmed cell death and this cytoprotective effect relies on its ability to catabolize free heme and prevent it from sensitizing cells to undergo apoptosis (PubMed:20055707).Heme oxygenase 1(Microbial infection) During SARS-COV-2 infection, promotes SARS-CoV-2 ORF3A-mediated autophagy but is unlikely to be required for ORF3A-mediated induction of reticulophagy.Heme oxygenase 1 soluble formCatalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron.

Alternative names

Recommended products

Rabbit Polyclonal Heme Oxygenase 1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Rat, Human, Mouse samples. Cited in 27 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HMOX1 aa 1 to C-terminus.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human HMOX1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P09601
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Heme Oxygenase 1 also known as HO-1 or HMOX1 is an enzyme that plays an important mechanistic role in heme catabolism. It catalyzes the degradation of heme into biliverdin carbon monoxide and free iron. This process involves the cleavage of the heme ring. HO-1 has a molecular weight of approximately 32 kDa. It is widely expressed in numerous tissues but is especially abundant in the liver and spleen. Its expression is induced by heme and other stress stimuli such as heavy metals cytokines and reactive oxygen species.

Biological function summary

Heme Oxygenase 1 serves important protective functions in the body. It is not part of a larger complex but its products such as carbon monoxide and biliverdin have their own biological activities. Carbon monoxide produced by HO-1 has antiflammatory properties and can modulate apoptotic pathways. Biliverdin is reduced to bilirubin which acts as an antioxidant. The enzyme therefore directly influences cellular stress responses and maintains cellular homeostasis through these processes.

Pathways

Heme Oxygenase 1 is integrally involved in oxidative stress response and heme metabolism. It participates in the cellular response to oxidative damage by reducing oxidative stress and promoting cytoprotection. Through its heme degradation activity it is connected with the synthesis of biologically active molecules like bilirubin and carbon monoxide. Heme Oxygenase 1 activity is related to other proteins in oxidative stress pathways such as Nuclear Factor Erythroid 2-Related Factor 2 (Nrf2) which regulates its expression and globins which are sources of heme for HO-1 activity.

Associated diseases and disorders

Heme Oxygenase 1 has been linked to conditions like cardiovascular diseases and neurodegenerative disorders. Its expression can attenuate the severity of atherosclerosis where oxidative stress is an important factor. In neurodegenerative diseases HO-1’s antioxidant properties may provide neuroprotection by mitigating oxidative damage. The protein's interactions with inflammatory cytokines such as Interleukin-6 and tumor necrosis factor-alpha influence its activity in these disease contexts.

Product promise

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14 product images

  • Western blot - Anti-Heme Oxygenase 1 antibody (ab137749), expandable thumbnail

    Western blot - Anti-Heme Oxygenase 1 antibody (ab137749)

    All lanes: Western blot - Anti-Heme Oxygenase 1 antibody (ab137749) at 1/500 dilution

    Lane 1: Wile-type HeLa cell lysate at 30 µg

    Lane 2: Knockout Hela cell lysates at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 33 kDa

    Observed band size: 33 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody (ab137749), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody (ab137749)

    Immunohistochemical analysis of human renal carcinaoma using ab137749 at 1/500.

  • Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody (ab137749), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody (ab137749)

    Immunofluorescent analysis of A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.
    Green: ab137749 diluted at 1:500.
    Blue: Hoechst 33342 staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody (ab137749), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody (ab137749)

    Immunohistochemical analysis of rat spleen using ab137749 at 1/500.

  • Western blot - Anti-Heme Oxygenase 1 antibody (ab137749), expandable thumbnail

    Western blot - Anti-Heme Oxygenase 1 antibody (ab137749)

    All lanes: Western blot - Anti-Heme Oxygenase 1 antibody (ab137749) at 1/5000 dilution

    Lane 1: Untreated HeLa whole cell extracts at 30 µg

    Lane 2: Treated HeLa whole cell extracts at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 33 kDa

  • Western blot - Anti-Heme Oxygenase 1 antibody (ab137749), expandable thumbnail

    Western blot - Anti-Heme Oxygenase 1 antibody (ab137749)

    12% SDS PAGE

    All lanes: Western blot - Anti-Heme Oxygenase 1 antibody (ab137749) at 1/1000 dilution

    Lane 1: H1299 cell lysate at 30 µg

    Lane 2: HeLa cell lysate at 30 µg

    Predicted band size: 33 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody (ab137749), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody (ab137749)

    Immunohistochemical analysis of paraffin-embedded OV90 xenograft, labelling Heme Oxygenase 1 using ab137749 at 1/100 dilution.

  • Western blot - Anti-Heme Oxygenase 1 antibody (ab137749), expandable thumbnail

    Western blot - Anti-Heme Oxygenase 1 antibody (ab137749)

    12% SDS PAGE

    All lanes: Western blot - Anti-Heme Oxygenase 1 antibody (ab137749) at 1/1000 dilution

    Lane 1: NIH-3T3 whole cell lysate at 30 µg

    Lane 2: JC whole cell lysate at 30 µg

    Lane 3: BCL1 whole cell lysate at 30 µg

    Lane 4: C2C12 whole cell lysate at 30 µg

    Lane 5: Raw 264.7 whole cell lysate at 30 µg

    Predicted band size: 33 kDa

  • Western blot - Anti-Heme Oxygenase 1 antibody (ab137749), expandable thumbnail

    Western blot - Anti-Heme Oxygenase 1 antibody (ab137749)

    Samples were seperated by 12% SDS-PAGE.

    All lanes: Western blot - Anti-Heme Oxygenase 1 antibody (ab137749) at 1/5000 dilution

    Lane 1: Untreated (–) HeLa whole cell extracts at 30 µg

    Lane 2: Treated (+) HeLa whole cell extracts at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 32 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody (ab137749), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody (ab137749)

    Immunofluorescence analysis of 4% paraformaldehyde fixed Mock and treated HeLa cells labelling Heme Oxygenase 1 protein at endoplasmic reticulum and nucleus using ab137749 at a 1/500 dilution at RT for 15 min (Green). The nuclear counter stain is DAPI (Blue).

  • Western blot - Anti-Heme Oxygenase 1 antibody (ab137749), expandable thumbnail

    Western blot - Anti-Heme Oxygenase 1 antibody (ab137749)

    Samples were seperated by 12% SDS-PAGE.

    All lanes: Western blot - Anti-Heme Oxygenase 1 antibody (ab137749) at 1/1000 dilution

    All lanes: Rat tissue extract at 50 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 32 kDa

  • Western blot - Anti-Heme Oxygenase 1 antibody (ab137749), expandable thumbnail

    Western blot - Anti-Heme Oxygenase 1 antibody (ab137749)

    Samples were seperated by 12% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

    All lanes: Western blot - Anti-Heme Oxygenase 1 antibody (ab137749) at 1/1000 dilution

    Lane 1: U87-MG whole cell extracts at 30 µg

    Lane 2: DU145 whole cell extracts at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 32 kDa

  • Western blot - Anti-Heme Oxygenase 1 antibody (ab137749), expandable thumbnail

    Western blot - Anti-Heme Oxygenase 1 antibody (ab137749)

    Samples were seperated by 12% SDS-PAGE.

    All lanes: Western blot - Anti-Heme Oxygenase 1 antibody (ab137749) at 1/1000 dilution

    All lanes: NIH-3T3 Whole cell extract at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 32 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody (ab137749), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody (ab137749)

    Paraffin-embedded rat spleen tissue stained for Heme Oxygenase 1 protein at cytoplasm with ab137749 at a 1/500 dilution in immunohistochemical analysis.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

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Product protocols

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