AB137749

Anti-Heme Oxygenase 1 antibody

Name: Anti-Heme Oxygenase 1 antibody (ab137749) | Abcam
Brand: Abcam Limited
SKU: AB137749
Price: 565 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(36 Publications)

Rabbit Polyclonal Heme Oxygenase 1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Rat, Human, Mouse samples. Cited in 36 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HMOX1 aa 1 to C-terminus.

View Alternative Names

HO, HO1, HMOX1, Heme oxygenase 1, HO-1

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody (AB137749)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody (AB137749)

Immunohistochemical analysis of human renal carcinaoma using ab137749 at 1/500.

Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody (AB137749)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody (AB137749)

Immunofluorescence analysis of 4% paraformaldehyde fixed Mock and treated HeLa cells labelling Heme Oxygenase 1 protein at endoplasmic reticulum and nucleus using ab137749 at a 1/500 dilution at RT for 15 min (Green). The nuclear counter stain is DAPI (Blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody (AB137749)

Paraffin-embedded rat spleen tissue stained for Heme Oxygenase 1 protein at cytoplasm with ab137749 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Supplier Data

Western blot - Anti-Heme Oxygenase 1 antibody (AB137749)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-Heme Oxygenase 1 antibody (ab137749) at 1/5000 dilution

Lane 1:

Untreated (–) HeLa whole cell extracts at 30 µg

Lane 2:

Treated (+) HeLa whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 32 kDa

false

Supplier Data

Western blot - Anti-Heme Oxygenase 1 antibody (AB137749)

Samples were separated by 12% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-Heme Oxygenase 1 antibody (ab137749) at 1/1000 dilution

Lane 1:

U87-MG whole cell extracts at 30 µg

Lane 2:

DU145 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 32 kDa

false

Supplier Data

Western blot - Anti-Heme Oxygenase 1 antibody (AB137749)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-Heme Oxygenase 1 antibody (ab137749) at 1/1000 dilution

All lanes:

NIH-3T3 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 32 kDa

false

Supplier Data

Western blot - Anti-Heme Oxygenase 1 antibody (AB137749)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-Heme Oxygenase 1 antibody (ab137749) at 1/1000 dilution

All lanes:

Rat tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human HMOX1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P09601

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Alternatively antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) may be used. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Alternatively antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) may be used. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Heme Oxygenase 1 also known as HO-1 or HMOX1 is an enzyme that plays an important mechanistic role in heme catabolism. It catalyzes the degradation of heme into biliverdin carbon monoxide and free iron. This process involves the cleavage of the heme ring. HO-1 has a molecular weight of approximately 32 kDa. It is widely expressed in numerous tissues but is especially abundant in the liver and spleen. Its expression is induced by heme and other stress stimuli such as heavy metals cytokines and reactive oxygen species.

Biological function summary

Heme Oxygenase 1 serves important protective functions in the body. It is not part of a larger complex but its products such as carbon monoxide and biliverdin have their own biological activities. Carbon monoxide produced by HO-1 has antiflammatory properties and can modulate apoptotic pathways. Biliverdin is reduced to bilirubin which acts as an antioxidant. The enzyme therefore directly influences cellular stress responses and maintains cellular homeostasis through these processes.

Pathways

Heme Oxygenase 1 is integrally involved in oxidative stress response and heme metabolism. It participates in the cellular response to oxidative damage by reducing oxidative stress and promoting cytoprotection. Through its heme degradation activity it is connected with the synthesis of biologically active molecules like bilirubin and carbon monoxide. Heme Oxygenase 1 activity is related to other proteins in oxidative stress pathways such as Nuclear Factor Erythroid 2-Related Factor 2 (Nrf2) which regulates its expression and globins which are sources of heme for HO-1 activity.

Heme Oxygenase 1 has been linked to conditions like cardiovascular diseases and neurodegenerative disorders. Its expression can attenuate the severity of atherosclerosis where oxidative stress is an important factor. In neurodegenerative diseases HO-1’s antioxidant properties may provide neuroprotection by mitigating oxidative damage. The protein's interactions with inflammatory cytokines such as Interleukin-6 and tumor necrosis factor-alpha influence its activity in these disease contexts.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Heme oxygenase 1. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron (PubMed : 11121422, PubMed : 19556236, PubMed : 7703255). Affords protection against programmed cell death and this cytoprotective effect relies on its ability to catabolize free heme and prevent it from sensitizing cells to undergo apoptosis (PubMed : 20055707).. Heme oxygenase 1. (Microbial infection) During SARS-COV-2 infection, promotes SARS-CoV-2 ORF3A-mediated autophagy but is unlikely to be required for ORF3A-mediated induction of reticulophagy.. Heme oxygenase 1 soluble form. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron.

See full target information HMOX1

style

left-column

Publications (36)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 87:103864 PubMed40972157

2025

Low-GPX4 drives a sustained drug-tolerant persister state in TNBC by a targetable adaptive FSP1 upregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Nazia Chaudhary,Dibita Mandal,Bhagya Shree Choudhary,Sushmita Patra,Darshan Jain,Pritam Poonia,Shagufa Shaikh,Siddhi Tekalkar,Shivani Malvankar,Anusha Shivashankar,Eeshrita Jog,Leena Pilankar,Rahul Thorat,Vaishali V Kailje,Sonal Khanna,Subhakankha Manna,Bushra K Khan,Anjana Jadhav,Kedar Sharma,Soundharya Ramu,Sarthak Sahoo,Mohit Kumar Jolly,Sorab N Dalal,Sanju Sinha,Nishanth Ulhas Nair,Eytan Ruppin,Fabienne Lamballe,Flavio Maina,Nandini Verma

Dose-response : a publication of International Hormesis Society 23:15593258251341593 PubMed40395975

2025

Nrf2 Deficiency Brings About Increased Sensitive to IR and 7,12-dimethylbenz(a)anthracene and Leukemia Predisposition.

Applications

Unspecified application

Species

Unspecified reactive species

Mingxin Dong,Ping Yang,Xinyu Zhang,Shasha Nie,Xiaohui Sun

Molecular medicine reports 32: PubMed40280116

2025

Understanding the role of iron/heme metabolism in the anti‑inflammatory effects of natural sulfur molecules against lipopolysaccharide‑induced inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Young Kang,Se Won Bae,Kyoung-Jin Jang

Oncology letters 29:155 PubMed39911153

2025

Construction of a lung adenocarcinoma prognostic model based on KEAP1/NRF2/HO‑1 mutation‑mediated upregulated genes and bioinformatic analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhu,Ye Zhang,Lingyun Yang,Lu Chen,Chaobo Chen,Qifeng Shi,Zipeng Xu

Phytotherapy research : PTR 38:5441-5457 PubMed39289784

2024

Geniposidic Acid Attenuates Chronic Tubulointerstitial Nephropathy Through Regulation of the NF-ƙB/Nrf2 Pathway Via Aryl Hydrocarbon Receptor Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Ni Wang,Xiao-Jun Li,Wen-Feng Wang,Liang Zou,Hua Miao,Ying-Yong Zhao

European journal of histochemistry : EJH 67: PubMed38058290

2023

Pre-treatment with galectin-1 attenuates lipopolysaccharide-induced myocarditis by regulating the Nrf2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Liying Shen,Kongjie Lu,Zhenfeng Chen,Yingwei Zhu,Cong Zhang,Li Zhang

Environmental toxicology and pharmacology 100:104138 PubMed37137421

2023

Disruption of the mouse liver epitranscriptome by long-term aroclor 1260 exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Kellianne M Piell,Belinda J Petri,Kimberly Z Head,Banrida Wahlang,Raobo Xu,Xiang Zhang,Jianmin Pan,Shesh N Rai,Kalpani de Silva,Julia H Chariker,Eric C Rouchka,Min Tan,Yan Li,Matthew C Cave,Carolyn M Klinge

Oxidative medicine and cellular longevity 2022:7957255 PubMed36092168

2022

Atorvastatin Attenuates Radiotherapy-Induced Intestinal Damage through Activation of Autophagy and Antioxidant Effects.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Feng Wei,Ching-Hsueh Cheng,Shu-Yu Wen,Jui-Chueh Lin,Yu-Hsuan Chen,Chun-Wei Wang,Yi-Hsuan Lee,Sung-Hsin Kuo

Evidence-based complementary and alternative medicine : eCAM 2021:5881321 PubMed34912465

2021

Shikonin Alleviates Endothelial Cell Injury Induced by ox-LDL via AMPK/Nrf2/HO-1 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shuang Liu,Wen Yan,Yanbing Hu,Huiying Wu

Frontiers in pharmacology 12:723988 PubMed34658862

2021

Betaine Attenuates Osteoarthritis by Inhibiting Osteoclastogenesis and Angiogenesis in Subchondral Bone.

Applications

Unspecified application

Species

Unspecified reactive species

Wang Yajun,Cui Jin,Gu Zhengrong,Fang Chao,Hu Yan,Weng Weizong,Li Xiaoqun,Zhou Qirong,Chen Huiwen,Zhang Hao,Guo Jiawei,Zhuang Xinchen,Sheng Shihao,Wang Sicheng,Chen Xiao,Su Jiacan

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Heme Oxygenase 1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody (AB137749)

Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody (AB137749)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody (AB137749)

Western blot - Anti-Heme Oxygenase 1 antibody (AB137749)

Western blot - Anti-Heme Oxygenase 1 antibody (AB137749)

Western blot - Anti-Heme Oxygenase 1 antibody (AB137749)

Western blot - Anti-Heme Oxygenase 1 antibody (AB137749)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (36)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise