Rabbit Polyclonal Heme Oxygenase 1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Rat, Human, Mouse samples. Cited in 27 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HMOX1 aa 1 to C-terminus.
IgG
Rabbit
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
IHC-P | WB | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Expected | Tested | Expected |
Rat | Tested | Tested | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/100 - 1/1000 | Notes Alternatively antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) may be used. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/100 - 1/1000 | Notes Alternatively antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) may be used. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
Species Rat | Dilution info 1/500 - 1/3000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
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Heme oxygenase 1Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron (PubMed:11121422, PubMed:19556236, PubMed:7703255). Affords protection against programmed cell death and this cytoprotective effect relies on its ability to catabolize free heme and prevent it from sensitizing cells to undergo apoptosis (PubMed:20055707).Heme oxygenase 1(Microbial infection) During SARS-COV-2 infection, promotes SARS-CoV-2 ORF3A-mediated autophagy but is unlikely to be required for ORF3A-mediated induction of reticulophagy.Heme oxygenase 1 soluble formCatalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron.
HO, HO1, HMOX1, HO, HO1, Heme oxygenase 1, HO-1
Rabbit Polyclonal Heme Oxygenase 1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Rat, Human, Mouse samples. Cited in 27 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HMOX1 aa 1 to C-terminus.
IgG
Rabbit
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
Heme Oxygenase 1 also known as HO-1 or HMOX1 is an enzyme that plays an important mechanistic role in heme catabolism. It catalyzes the degradation of heme into biliverdin carbon monoxide and free iron. This process involves the cleavage of the heme ring. HO-1 has a molecular weight of approximately 32 kDa. It is widely expressed in numerous tissues but is especially abundant in the liver and spleen. Its expression is induced by heme and other stress stimuli such as heavy metals cytokines and reactive oxygen species.
Heme Oxygenase 1 serves important protective functions in the body. It is not part of a larger complex but its products such as carbon monoxide and biliverdin have their own biological activities. Carbon monoxide produced by HO-1 has antiflammatory properties and can modulate apoptotic pathways. Biliverdin is reduced to bilirubin which acts as an antioxidant. The enzyme therefore directly influences cellular stress responses and maintains cellular homeostasis through these processes.
Heme Oxygenase 1 is integrally involved in oxidative stress response and heme metabolism. It participates in the cellular response to oxidative damage by reducing oxidative stress and promoting cytoprotection. Through its heme degradation activity it is connected with the synthesis of biologically active molecules like bilirubin and carbon monoxide. Heme Oxygenase 1 activity is related to other proteins in oxidative stress pathways such as Nuclear Factor Erythroid 2-Related Factor 2 (Nrf2) which regulates its expression and globins which are sources of heme for HO-1 activity.
Heme Oxygenase 1 has been linked to conditions like cardiovascular diseases and neurodegenerative disorders. Its expression can attenuate the severity of atherosclerosis where oxidative stress is an important factor. In neurodegenerative diseases HO-1’s antioxidant properties may provide neuroprotection by mitigating oxidative damage. The protein's interactions with inflammatory cytokines such as Interleukin-6 and tumor necrosis factor-alpha influence its activity in these disease contexts.
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All lanes: Western blot - Anti-Heme Oxygenase 1 antibody (ab137749) at 1/500 dilution
Lane 1: Wile-type HeLa cell lysate at 30 µg
Lane 2: Knockout Hela cell lysates at 30 µg
All lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 33 kDa
Observed band size: 33 kDa
Immunohistochemical analysis of human renal carcinaoma using ab137749 at 1/500.
Immunofluorescent analysis of A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ab137749 diluted at 1:500.
Blue: Hoechst 33342 staining.
Immunohistochemical analysis of rat spleen using ab137749 at 1/500.
All lanes: Western blot - Anti-Heme Oxygenase 1 antibody (ab137749) at 1/5000 dilution
Lane 1: Untreated HeLa whole cell extracts at 30 µg
Lane 2: Treated HeLa whole cell extracts at 30 µg
All lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 33 kDa
12% SDS PAGE
All lanes: Western blot - Anti-Heme Oxygenase 1 antibody (ab137749) at 1/1000 dilution
Lane 1: H1299 cell lysate at 30 µg
Lane 2: HeLa cell lysate at 30 µg
Predicted band size: 33 kDa
Immunohistochemical analysis of paraffin-embedded OV90 xenograft, labelling Heme Oxygenase 1 using ab137749 at 1/100 dilution.
12% SDS PAGE
All lanes: Western blot - Anti-Heme Oxygenase 1 antibody (ab137749) at 1/1000 dilution
Lane 1: NIH-3T3 whole cell lysate at 30 µg
Lane 2: JC whole cell lysate at 30 µg
Lane 3: BCL1 whole cell lysate at 30 µg
Lane 4: C2C12 whole cell lysate at 30 µg
Lane 5: Raw 264.7 whole cell lysate at 30 µg
Predicted band size: 33 kDa
Samples were seperated by 12% SDS-PAGE.
All lanes: Western blot - Anti-Heme Oxygenase 1 antibody (ab137749) at 1/5000 dilution
Lane 1: Untreated (–) HeLa whole cell extracts at 30 µg
Lane 2: Treated (+) HeLa whole cell extracts at 30 µg
All lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 32 kDa
Immunofluorescence analysis of 4% paraformaldehyde fixed Mock and treated HeLa cells labelling Heme Oxygenase 1 protein at endoplasmic reticulum and nucleus using ab137749 at a 1/500 dilution at RT for 15 min (Green). The nuclear counter stain is DAPI (Blue).
Samples were seperated by 12% SDS-PAGE.
All lanes: Western blot - Anti-Heme Oxygenase 1 antibody (ab137749) at 1/1000 dilution
All lanes: Rat tissue extract at 50 µg
All lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 32 kDa
Samples were seperated by 12% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
All lanes: Western blot - Anti-Heme Oxygenase 1 antibody (ab137749) at 1/1000 dilution
Lane 1: U87-MG whole cell extracts at 30 µg
Lane 2: DU145 whole cell extracts at 30 µg
All lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 32 kDa
Samples were seperated by 12% SDS-PAGE.
All lanes: Western blot - Anti-Heme Oxygenase 1 antibody (ab137749) at 1/1000 dilution
All lanes: NIH-3T3 Whole cell extract at 30 µg
All lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 32 kDa
Paraffin-embedded rat spleen tissue stained for Heme Oxygenase 1 protein at cytoplasm with ab137749 at a 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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