AB82585

Anti-Heme Oxygenase 1 antibody

Name: Anti-Heme Oxygenase 1 antibody (ab82585) | Abcam
Brand: Abcam Limited
SKU: AB82585
Price: 535 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal Heme Oxygenase 1 antibody. Suitable for WB and reacts with Human, Rat, Mouse, Dog, Recombinant full length protein - Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human HMOX1.

View Alternative Names

HO, HO1, HMOX1, Heme oxygenase 1, HO-1

1 Images

Unknown

Western blot - Anti-Heme Oxygenase 1 antibody (AB82585)

All lanes:

Western blot - Anti-Heme Oxygenase 1 antibody (ab82585) at 1/1000 dilution

Lane 1:

Heme Oxygenase 1 Recombinant Rat Protein

Lane 2:

Heme Oxygenase 1 Recombinant Human Protein

Lane 3:

Heme Oxygenase 2 Recombinant Human Protein

Lane 4:

Dog Liver Microsome Extract

Lane 5:

Mouse Liver Microsome Extract

Lane 6:

Rat Liver Microsome Extract

Lane 7:

Human Liver Microsome Extract

Predicted band size: 33 kDa

Observed band size: 33 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Dog, Human

Applications

applications

Immunogen

Synthetic Peptide within Human HMOX1. The exact immunogen used to generate this antibody is proprietary information.

P09601

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Heme Oxygenase 1 also known as HO-1 or HMOX1 is an enzyme that plays an important mechanistic role in heme catabolism. It catalyzes the degradation of heme into biliverdin carbon monoxide and free iron. This process involves the cleavage of the heme ring. HO-1 has a molecular weight of approximately 32 kDa. It is widely expressed in numerous tissues but is especially abundant in the liver and spleen. Its expression is induced by heme and other stress stimuli such as heavy metals cytokines and reactive oxygen species.

Biological function summary

Heme Oxygenase 1 serves important protective functions in the body. It is not part of a larger complex but its products such as carbon monoxide and biliverdin have their own biological activities. Carbon monoxide produced by HO-1 has antiflammatory properties and can modulate apoptotic pathways. Biliverdin is reduced to bilirubin which acts as an antioxidant. The enzyme therefore directly influences cellular stress responses and maintains cellular homeostasis through these processes.

Pathways

Heme Oxygenase 1 is integrally involved in oxidative stress response and heme metabolism. It participates in the cellular response to oxidative damage by reducing oxidative stress and promoting cytoprotection. Through its heme degradation activity it is connected with the synthesis of biologically active molecules like bilirubin and carbon monoxide. Heme Oxygenase 1 activity is related to other proteins in oxidative stress pathways such as Nuclear Factor Erythroid 2-Related Factor 2 (Nrf2) which regulates its expression and globins which are sources of heme for HO-1 activity.

Heme Oxygenase 1 has been linked to conditions like cardiovascular diseases and neurodegenerative disorders. Its expression can attenuate the severity of atherosclerosis where oxidative stress is an important factor. In neurodegenerative diseases HO-1’s antioxidant properties may provide neuroprotection by mitigating oxidative damage. The protein's interactions with inflammatory cytokines such as Interleukin-6 and tumor necrosis factor-alpha influence its activity in these disease contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Heme oxygenase 1. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron (PubMed : 11121422, PubMed : 19556236, PubMed : 7703255). Affords protection against programmed cell death and this cytoprotective effect relies on its ability to catabolize free heme and prevent it from sensitizing cells to undergo apoptosis (PubMed : 20055707).. Heme oxygenase 1. (Microbial infection) During SARS-COV-2 infection, promotes SARS-CoV-2 ORF3A-mediated autophagy but is unlikely to be required for ORF3A-mediated induction of reticulophagy.. Heme oxygenase 1 soluble form. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron.

See full target information HMOX1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular biosciences 10:1156062 PubMed37065442

2023

Susceptibility of cervical cancer to dihydroartemisinin-induced ferritinophagy-dependent ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hanqiang Shi,Lie Xiong,Guang Yan,Shuqin Du,Jie Liu,Yanbo Shi

BMC gastroenterology 19:25 PubMed30736744

2019

Hydrogen-rich solution attenuates cold ischemia-reperfusion injury in rat liver transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Keiichi Uto,Seisuke Sakamoto,Weitao Que,Keita Shimata,Shintaro Hashimoto,Masataka Sakisaka,Yasuko Narita,Daiki Yoshii,Lin Zhong,Yoshihiro Komohara,Xiao-Kang Li,Yukihiro Inomata,Taizo Hibi

Experimental and therapeutic medicine 16:1079-1086 PubMed30116359

2018

Effects of ginsenoside Rb1 on oxidative stress injury in rat spinal cords by regulating the eNOS/Nrf2/HO-1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xinwei Liu,Xiaochuan Gu,Miaomiao Yu,Ying Zi,Hailong Yu,Yu Wang,Yanchun Xie,Liangbi Xiang

Naunyn-Schmiedeberg's archives of pharmacology 391:371-383 PubMed29354880

2018

Endothelin-1-induced hypertrophic alterations and heme oxygenase-1 expression in cardiomyoblasts are counteracted by beta estradiol: in vitro and in vivo studies.

Applications

Species

Rat

Tunde Barta,Agnes Tosaki,David Haines,Gyorgy Balla,Istvan Lekli,Arpad Tosaki

Molecules (Basel, Switzerland) 20:22395-410 PubMed26694327

2015

Retinoprotective Effects of Bilberry Anthocyanins via Antioxidant, Anti-Inflammatory, and Anti-Apoptotic Mechanisms in a Visible Light-Induced Retinal Degeneration Model in Pigmented Rabbits.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Wang,Liang Zhao,Feng Lu,Xue Yang,Qianchun Deng,Baoping Ji,Fenghong Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Heme Oxygenase 1 antibody

Western blot - Anti-Heme Oxygenase 1 antibody (AB82585)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise