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Rabbit Recombinant Monoclonal Heme Oxygenase 1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 194 publications.


Images

Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (AB68477), expandable thumbnail
  • Immunoprecipitation - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (AB68477), expandable thumbnail
  • Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (AB68477), expandable thumbnail
  • Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (AB68477), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (AB68477), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Not recommended
Tested
Tested
Mouse
Expected
Tested
Not recommended
Expected
Expected
Rat
Expected
Tested
Not recommended
Expected
Expected

Tested
Tested

Species
Human
Dilution info
1/20
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/20000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/20000
Notes

-

Species
Human
Dilution info
1/1000 - 1/20000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Heme oxygenase 1. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron (PubMed:11121422, PubMed:19556236, PubMed:7703255). Affords protection against programmed cell death and this cytoprotective effect relies on its ability to catabolize free heme and prevent it from sensitizing cells to undergo apoptosis (PubMed:20055707). Heme oxygenase 1. (Microbial infection) During SARS-COV-2 infection, promotes SARS-CoV-2 ORF3A-mediated autophagy but is unlikely to be required for ORF3A-mediated induction of reticulophagy. Heme oxygenase 1 soluble form. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron.

Alternative names

HO, HO1, HMOX1, Heme oxygenase 1, HO-1

Recommended products

Rabbit Recombinant Monoclonal Heme Oxygenase 1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 194 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR1390Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Heme Oxygenase 1 also known as HO-1 or HMOX1 is an enzyme that plays an important mechanistic role in heme catabolism. It catalyzes the degradation of heme into biliverdin carbon monoxide and free iron. This process involves the cleavage of the heme ring. HO-1 has a molecular weight of approximately 32 kDa. It is widely expressed in numerous tissues but is especially abundant in the liver and spleen. Its expression is induced by heme and other stress stimuli such as heavy metals cytokines and reactive oxygen species.

Biological function summary

Heme Oxygenase 1 serves important protective functions in the body. It is not part of a larger complex but its products such as carbon monoxide and biliverdin have their own biological activities. Carbon monoxide produced by HO-1 has antiflammatory properties and can modulate apoptotic pathways. Biliverdin is reduced to bilirubin which acts as an antioxidant. The enzyme therefore directly influences cellular stress responses and maintains cellular homeostasis through these processes.

Pathways

Heme Oxygenase 1 is integrally involved in oxidative stress response and heme metabolism. It participates in the cellular response to oxidative damage by reducing oxidative stress and promoting cytoprotection. Through its heme degradation activity it is connected with the synthesis of biologically active molecules like bilirubin and carbon monoxide. Heme Oxygenase 1 activity is related to other proteins in oxidative stress pathways such as Nuclear Factor Erythroid 2-Related Factor 2 (Nrf2) which regulates its expression and globins which are sources of heme for HO-1 activity.

Associated diseases and disorders

Heme Oxygenase 1 has been linked to conditions like cardiovascular diseases and neurodegenerative disorders. Its expression can attenuate the severity of atherosclerosis where oxidative stress is an important factor. In neurodegenerative diseases HO-1’s antioxidant properties may provide neuroprotection by mitigating oxidative damage. The protein's interactions with inflammatory cytokines such as Interleukin-6 and tumor necrosis factor-alpha influence its activity in these disease contexts.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477), expandable thumbnail

    Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)

    Lanes 1 - 7: Merged signal (red and green). Green - ab68477 observed at 33 kDa. Red - loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

    ab68477 was shown to react with Heme Oxygenase 1 in wild-type A549 cells in Western blot with loss of signal observed in HMOX1 knockout cell line Human HMOX1 (Heme Oxygenase 1) knockout A549 cell line ab269503 (knockout cell lysate Human HMOX1 (Heme Oxygenase 1) knockout A549 cell lysate ab269665). Wild-type A549 and HMOX1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab68477 and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477) at 1/10000 dilution

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 2: HMOX1 knockout A549 cell lysate at 20 µg

    Lane 2: Western blot - Human HMOX1 (Heme Oxygenase 1) knockout A549 cell line (Human HMOX1 (Heme Oxygenase 1) knockout A549 cell line ab269503)

    Lane 3: Human Spleen tissue lysate at 20 µg

    Lane 4: HL-60 cell lysate at 20 µg

    Lane 5: MCF7 cell lysate at 20 µg

    Lane 6: HeLa cell lysate at 20 µg

    Lane 7: A549 cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 33 kDa

    Observed band size: 33 kDa

  • Immunoprecipitation - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477), expandable thumbnail

    Immunoprecipitation - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)

    ab68477 (purified) at 1/20 immunoprecipitating Heme Oxygenase 1 in A549 (Human lung carcinoma cell line) whole cell lysate.

    Lane 1 (input): A549 whole cell lysate (10ug).

    Lane 2 (+): ab68477 + A549 whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Syntenin antibody [EPR8102] ab133267 in HeLa whole cell lysate.

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Immunoprecipitation - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)

    Predicted band size: 33 kDa

    Observed band size: 33 kDa

  • Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477), expandable thumbnail

    Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)

    Hek293 & HL60 presumed negative or very low expression.

    Loading control GAPDH at 38kDa

    All lanes: Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477) at 1/1000 dilution

    Lane 1: Hek293 at 10 µg

    Lane 2: HL60 at 10 µg

    Lane 3: HeLa at 10 µg

    Lane 4: A549 at 10 µg

    Lane 5: Hu spleen at 10 µg

    Lane 6: Ms spleen at 10 µg

    Lane 7: Rt spleen at 10 µg

    Secondary

    All lanes: IRDye® 800CW Goat anti Rabbit

    Predicted band size: 33 kDa

    Observed band size: 32 kDa

  • Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477), expandable thumbnail

    Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)

    We are unsure how to define the extra bands.

    All lanes: Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477) at 1/1000 dilution

    Lane 1: HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates at 20 µg with 5% NFDM/TBST

    Lane 2: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 20 µg with 5% NFDM/TBST

    Lane 3: A549 (Human lung carcinoma epithelial cell) whole cell lysates at 20 µg with 5% NFDM/TBST

    Lane 4: Mouse spleen lysates at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 33 kDa

    Observed band size: 33 kDa

    Exposure time: 10s

  • Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)

    Immunocytochemistry/Immunofluorescence analysis of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Heme Oxygenase 1 with purified ab68477 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. A goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

    Negative control 1: PBS only.

  • Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477), expandable thumbnail

    Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)

    Blocking and dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477) at 1/20000 dilution

    Lane 1: Rat kidney lysate at 20 µg

    Lane 2: Rat spleen lysate at 20 µg

    Lane 3: Mouse kidney lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 33 kDa

    Observed band size: 33 kDa

  • Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)

    Intracellular Flow Cytometry analysis of A549 (human lung carcinoma) cells labeling with purified ab68477 at 1/200 dilution (1ug/ml) (Red). Cells were fixed with4% paraformaldehydeand permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) (1/2000 dilution) was used as the secondary antibody.Rabbit monoclonal IgG (Black) (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) was used as a isotype control.Cell without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

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