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Knockout Tested Rabbit Monoclonal Heme Oxygenase 1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 53 publications.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (AB189491), expandable thumbnail
  • Western blot - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (AB189491), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (AB189491), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (AB189491), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (AB189491), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Expected
Tested
Tested
Tested
Tested
Mouse
Tested
Tested
Tested
Tested
Tested
Rat
Expected
Tested
Expected
Expected
Tested

Tested
Tested

Species

Mouse

Dilution info

1/30

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/2000

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/250

Notes

-

Species

Human

Dilution info

1/250

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/50

Notes

-

Species

Human

Dilution info

1/50

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/20000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/20000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/20000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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Target data

Function

Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.

Alternative names

Heme oxygenase 1, HO-1, HMOX1, HO, HO1

Recommended products

Knockout Tested Rabbit Monoclonal Heme Oxygenase 1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 53 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR18161-128

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Heme Oxygenase 1 also known as HO-1 or HMOX1 is an enzyme that plays an important mechanistic role in heme catabolism. It catalyzes the degradation of heme into biliverdin carbon monoxide and free iron. This process involves the cleavage of the heme ring. HO-1 has a molecular weight of approximately 32 kDa. It is widely expressed in numerous tissues but is especially abundant in the liver and spleen. Its expression is induced by heme and other stress stimuli such as heavy metals cytokines and reactive oxygen species.

Biological function summary

Heme Oxygenase 1 serves important protective functions in the body. It is not part of a larger complex but its products such as carbon monoxide and biliverdin have their own biological activities. Carbon monoxide produced by HO-1 has antiflammatory properties and can modulate apoptotic pathways. Biliverdin is reduced to bilirubin which acts as an antioxidant. The enzyme therefore directly influences cellular stress responses and maintains cellular homeostasis through these processes.

Pathways

Heme Oxygenase 1 is integrally involved in oxidative stress response and heme metabolism. It participates in the cellular response to oxidative damage by reducing oxidative stress and promoting cytoprotection. Through its heme degradation activity it is connected with the synthesis of biologically active molecules like bilirubin and carbon monoxide. Heme Oxygenase 1 activity is related to other proteins in oxidative stress pathways such as Nuclear Factor Erythroid 2-Related Factor 2 (Nrf2) which regulates its expression and globins which are sources of heme for HO-1 activity.

Associated diseases and disorders

Heme Oxygenase 1 has been linked to conditions like cardiovascular diseases and neurodegenerative disorders. Its expression can attenuate the severity of atherosclerosis where oxidative stress is an important factor. In neurodegenerative diseases HO-1’s antioxidant properties may provide neuroprotection by mitigating oxidative damage. The protein's interactions with inflammatory cytokines such as Interleukin-6 and tumor necrosis factor-alpha influence its activity in these disease contexts.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)

    Immunohistochemical analysis of paraffin embedded mouse liver tissue labeling Heme Oxygenase 1 with ab189491 at 1/20000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on Kupffer cells of mouse liver (PMID: 9449694) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491), expandable thumbnail

    Western blot - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)

    Blocking: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 18400743).

    The lower band observed is a truncated form of Heme Oxygenase 1 (PMID: 17430897).

    All lanes: Western blot - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491) at 1/2000 dilution

    All lanes: Human spleen lysate at 10 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/4000 dilution

    Predicted band size: 33 kDa

    Observed band size: 28 kDa, 32 kDa

    Exposure time: 3min

  • Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)

    Immunofluorescent analysis of 4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling Heme Oxygenase 1 with ab189491 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cells. Details of counterstains: Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution; DAPI for nuclei.
    The negative controls are as follows: Secondary antibody only for control.

  • Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)

    Immunofluorescent analysis of 4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labeling Heme Oxygenase 1 with ab189491 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on NIH/3T3 cells. Details of counterstains: Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution; DAPI for nuclei.
    The negative controls are as follows: Secondary antibody only for control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)

    Immunohistochemical analysis of paraffin embedded human liver tissue labeling Heme Oxygenase 1 with ab189491 at 1/20,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on Kupffer cells of human liver (PMID: 9449694) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)

    Immunohistochemical analysis of paraffin embedded rat liver tissue labeling Heme Oxygenase 1 with ab189491 at 1/20,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on Kupffer cells of rat liver (PMID: 9449694) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunoprecipitation - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491), expandable thumbnail

    Immunoprecipitation - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)

    Heme Oxygenase 1 was immunoprecipitated from 0.35 mg of NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab189491 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab189491 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution.
    Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 μg (Input).
    Lane 2: NIH/3T3 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab189491 in NIH/3T3 whole cell lysate (-).
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    The truncated form of Heme Oxygenase 1 is described in the literature (PMID: 17430897).

    All lanes: Immunoprecipitation - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)

    Predicted band size: 33 kDa

    Observed band size: 28 kDa, 32 kDa

    Exposure time: 1s

  • Western blot - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491), expandable thumbnail

    Western blot - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)

    Blocking: 5% NFDM/TBST.

    Exposure time:

    Lanes 1,2 and 3: 2 seconds;

    Lane 4: 1 second

    The molecular weight observed is consistent with what has been described in the literature (PMID: 18400743).

    The lower band observed is a truncated form of Heme Oxygenase 1 (PMID: 17430897).

    All lanes: Western blot - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491) at 1/5000 dilution

    Lane 1: NIH/3T3 (mouse embryonic fibroblast) lysate at 20 µg

    Lane 2: Rat spleen lysate at 20 µg

    Lane 3: HeLa (human cervix adenocarcinoma epithelial cell) lysate at 20 µg

    Lane 4: Mouse spleen lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 33 kDa

    Observed band size: 28 kDa, 32 kDa

  • Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)

    Intracellular flow cytometric analysis of 90% methanol/ 4% paraformaldehyde fixed NIH/3T3 (mouse embryonic fibroblast) cell line labeling Heme Oxygenase1 with ab189491 at 1/50 (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluorr® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)

    Intracellular flow cytometric analysis of 90% methanol/4% paraformaldehyde fixed HeLa (human cervix adenocarcinoma epithelial cell) cell line labeling Heme Oxygenase1 with ab189491 at 1/50 (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluorr® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491), expandable thumbnail

    Western blot - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491)

    False colour image of Western blot: Anti-Heme Oxygenase 1 antibody [EPR18161-128] staining at 1/2000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab189491 was shown to bind specifically to Heme Oxygenase 1. A band was observed at 32 kDa in wild-type A549 cell lysates with no signal observed at this size in HMOX1 knockout cell line Human HMOX1 (Heme Oxygenase 1) knockout A549 cell line ab269503 (HMOX knockout A549 lysate ab259782).
    To generate this image, wild-type and HMOX1 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween®20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    Lanes 1 - 4: Western blot - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free ab224677) at 1/2000 dilution

    Lanes 1 - 5: Western blot - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (ab189491) at 1/2000 dilution

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 2: HMOX1 knockout A549 cell lysate at 20 µg

    Lane 3: Mouse Spleen cell lysate at 20 µg

    Lane 4: HeLa cell lysate at 20 µg

    Lane 5: HEK-293 cell lysate at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 32 kDa

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Product protocols

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