Rabbit Monoclonal Heme Oxygenase 1 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human, Rat samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (AB224677), expandable thumbnail](https://content.abcam.com/products/images/heme-oxygenase-1-antibody-epr18161-128-bsa-and-azide-free-ab224677--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img76702.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (AB224677)")
![Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (AB224677), expandable thumbnail](https://content.abcam.com/products/images/heme-oxygenase-1-antibody-epr18161-128-bsa-and-azide-free-ab224677--immunocytochemistry-immunofluorescence-img69755.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (AB224677)")
![Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (AB224677), expandable thumbnail](https://content.abcam.com/products/images/heme-oxygenase-1-antibody-epr18161-128-bsa-and-azide-free-ab224677--immunocytochemistry-immunofluorescence-img109915.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (AB224677)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (AB224677), expandable thumbnail](https://content.abcam.com/products/images/heme-oxygenase-1-antibody-epr18161-128-bsa-and-azide-free-ab224677--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img90261.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (AB224677)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (AB224677), expandable thumbnail](https://content.abcam.com/products/images/heme-oxygenase-1-antibody-epr18161-128-bsa-and-azide-free-ab224677--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img3751.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (AB224677)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Human | Expected | Expected | Expected | Tested | Expected |
| Mouse | Tested | Expected | Tested | Expected | Expected |
| Rat | Expected | Expected | Expected | Expected | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Product
4 products for Alternative Version
Target data
Function
Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.
Alternative names
Heme oxygenase 1, HO-1, HMOX1, HO, HO1
Recommended products
Rabbit Monoclonal Heme Oxygenase 1 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human, Rat samples.
Alternative names
Heme oxygenase 1, HO-1, HMOX1, HO, HO1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR18161-128
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab224677 is the carrier-free version of Anti-Heme Oxygenase 1 antibody [EPR18161-128] ab189491.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- Activity summary
Heme Oxygenase 1 also known as HO-1 or HMOX1 is an enzyme that plays an important mechanistic role in heme catabolism. It catalyzes the degradation of heme into biliverdin carbon monoxide and free iron. This process involves the cleavage of the heme ring. HO-1 has a molecular weight of approximately 32 kDa. It is widely expressed in numerous tissues but is especially abundant in the liver and spleen. Its expression is induced by heme and other stress stimuli such as heavy metals cytokines and reactive oxygen species.
- Biological function summary
Heme Oxygenase 1 serves important protective functions in the body. It is not part of a larger complex but its products such as carbon monoxide and biliverdin have their own biological activities. Carbon monoxide produced by HO-1 has antiflammatory properties and can modulate apoptotic pathways. Biliverdin is reduced to bilirubin which acts as an antioxidant. The enzyme therefore directly influences cellular stress responses and maintains cellular homeostasis through these processes.
- Pathways
Heme Oxygenase 1 is integrally involved in oxidative stress response and heme metabolism. It participates in the cellular response to oxidative damage by reducing oxidative stress and promoting cytoprotection. Through its heme degradation activity it is connected with the synthesis of biologically active molecules like bilirubin and carbon monoxide. Heme Oxygenase 1 activity is related to other proteins in oxidative stress pathways such as Nuclear Factor Erythroid 2-Related Factor 2 (Nrf2) which regulates its expression and globins which are sources of heme for HO-1 activity.
- Associated diseases and disorders
Heme Oxygenase 1 has been linked to conditions like cardiovascular diseases and neurodegenerative disorders. Its expression can attenuate the severity of atherosclerosis where oxidative stress is an important factor. In neurodegenerative diseases HO-1’s antioxidant properties may provide neuroprotection by mitigating oxidative damage. The protein's interactions with inflammatory cytokines such as Interleukin-6 and tumor necrosis factor-alpha influence its activity in these disease contexts.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
9 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (ab224677), expandable thumbnail](https://content.abcam.com/products/images/heme-oxygenase-1-antibody-epr18161-128-bsa-and-azide-free-ab224677--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img76702.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (ab224677)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (ab224677)
Immunohistochemical analysis of paraffin embedded mouse liver tissue labeling Heme Oxygenase 1 with Anti-Heme Oxygenase 1 antibody [EPR18161-128] ab189491 at 1/20000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on Kupffer cells of mouse liver (PMID: 9449694) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Heme Oxygenase 1 antibody [EPR18161-128] ab189491).
![Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (ab224677), expandable thumbnail](https://content.abcam.com/products/images/heme-oxygenase-1-antibody-epr18161-128-bsa-and-azide-free-ab224677--immunocytochemistry-immunofluorescence-img69755.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (ab224677)")
Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (ab224677)
Immunofluorescent analysis of 4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling Heme Oxygenase 1 with Anti-Heme Oxygenase 1 antibody [EPR18161-128] ab189491 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cells. Details of counterstains: Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution; DAPI for nuclei.
The negative controls are as follows: Secondary antibody only for control.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Heme Oxygenase 1 antibody [EPR18161-128] ab189491).
![Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (ab224677), expandable thumbnail](https://content.abcam.com/products/images/heme-oxygenase-1-antibody-epr18161-128-bsa-and-azide-free-ab224677--immunocytochemistry-immunofluorescence-img109915.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (ab224677)")
Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (ab224677)
Immunofluorescent analysis of 4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labeling Heme Oxygenase 1 with Anti-Heme Oxygenase 1 antibody [EPR18161-128] ab189491 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on NIH/3T3 cells. Details of counterstains: Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution; DAPI for nuclei.
The negative controls are as follows: Secondary antibody only for control.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Heme Oxygenase 1 antibody [EPR18161-128] ab189491).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (ab224677), expandable thumbnail](https://content.abcam.com/products/images/heme-oxygenase-1-antibody-epr18161-128-bsa-and-azide-free-ab224677--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img90261.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (ab224677)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (ab224677)
Immunohistochemical analysis of paraffin embedded human liver tissue labeling Heme Oxygenase 1 with Anti-Heme Oxygenase 1 antibody [EPR18161-128] ab189491 at 1/20000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on Kupffer cells of human liver (PMID: 9449694) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Heme Oxygenase 1 antibody [EPR18161-128] ab189491).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (ab224677), expandable thumbnail](https://content.abcam.com/products/images/heme-oxygenase-1-antibody-epr18161-128-bsa-and-azide-free-ab224677--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img3751.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (ab224677)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (ab224677)
Immunohistochemical analysis of paraffin embedded rat liver tissue labeling Heme Oxygenase 1 with Anti-Heme Oxygenase 1 antibody [EPR18161-128] ab189491 at 1/20,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on Kupffer cells of rat liver (PMID: 9449694) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Heme Oxygenase 1 antibody [EPR18161-128] ab189491).
![Immunoprecipitation - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (ab224677), expandable thumbnail](https://content.abcam.com/products/images/heme-oxygenase-1-antibody-epr18161-128-bsa-and-azide-free-ab224677--immunoprecipitation-img12229.jpg "Immunoprecipitation - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (ab224677)")
Immunoprecipitation - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (ab224677)
Heme Oxygenase 1 was immunoprecipitated from 0.35 mg of NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with Anti-Heme Oxygenase 1 antibody [EPR18161-128] ab189491 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-Heme Oxygenase 1 antibody [EPR18161-128] ab189491 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution.
Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 μg (Input).
Lane 2: NIH/3T3 whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Heme Oxygenase 1 antibody [EPR18161-128] ab189491 in NIH/3T3 whole cell lysate (-).
Blocking and dilution buffer and concentration: 5% NFDM/TBST.The truncated form of Heme Oxygenase 1 is described in the literature (PMID: 17430897).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Heme Oxygenase 1 antibody [EPR18161-128] ab189491).
All lanes: Immunoprecipitation - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (Anti-Heme Oxygenase 1 antibody [EPR18161-128] ab189491)
Predicted band size: 33 kDa
Observed band size: 28 kDa, 32 kDa
Exposure time: 1s
![Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (ab224677), expandable thumbnail](https://content.abcam.com/products/images/heme-oxygenase-1-antibody-epr18161-128-bsa-and-azide-free-ab224677--flow-cytometry-intracellular-img37362.jpg "Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (ab224677)")
Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (ab224677)
Intracellular flow cytometric analysis of 90% methanol/ 4% paraformaldehyde fixed NIH/3T3 (mouse embryonic fibroblast) cell line labeling Heme Oxygenase 1 with Anti-Heme Oxygenase 1 antibody [EPR18161-128] ab189491 at 1/50 (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Heme Oxygenase 1 antibody [EPR18161-128] ab189491).
![Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (ab224677), expandable thumbnail](https://content.abcam.com/products/images/heme-oxygenase-1-antibody-epr18161-128-bsa-and-azide-free-ab224677--flow-cytometry-intracellular-img26824.jpg "Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (ab224677)")
Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (ab224677)
Intracellular flow cytometric analysis of 90% methanol/4% paraformaldehyde fixed HeLa (human cervix adenocarcinoma epithelial cell) cell line labeling Heme Oxygenase 1 with Anti-Heme Oxygenase 1 antibody [EPR18161-128] ab189491 at 1/50 (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Heme Oxygenase 1 antibody [EPR18161-128] ab189491).
![Western blot - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (ab224677), expandable thumbnail](https://content.abcam.com/products/images/heme-oxygenase-1-antibody-epr18161-128-bsa-and-azide-free-ab224677--western-blot-img417807.jpg "Western blot - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (ab224677)")
Western blot - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (ab224677)
This data was developed using the same antibody clone in a different buffer formulation (Anti-Heme Oxygenase 1 antibody [EPR18161-128] ab189491).
False colour image of Western blot: Anti-Heme Oxygenase 1 antibody [EPR18161-128] staining at 1/2000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-Heme Oxygenase 1 antibody [EPR18161-128] ab189491 was shown to bind specifically to Heme Oxygenase 1. A band was observed at 32 kDa in wild-type A549 cell lysates with no signal observed at this size in HMOX1 knockout cell line Human HMOX1 (Heme Oxygenase 1) knockout A549 cell line ab269503 (HMOX knockout A549 lysate ab259782).
To generate this image, wild-type and HMOX1 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween®20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.Lanes 1 - 4: Western blot - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (ab224677) at 1/2000 dilution
Lanes 1 - 5: Western blot - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (Anti-Heme Oxygenase 1 antibody [EPR18161-128] ab189491) at 1/2000 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: HMOX1 knockout A549 cell lysate at 20 µg
Lane 3: Mouse Spleen cell lysate at 20 µg
Lane 4: HeLa cell lysate at 20 µg
Lane 5: HEK-293 cell lysate at 20 µg
SecondaryAll lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 32 kDa
![Immunoprecipitation - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (ab224677), expandable thumbnail](https://content.abcam.com/products/images/heme-oxygenase-1-antibody-epr18161-128-bsa-and-azide-free-ab224677--immunoprecipitation-img12229.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (ab224677)")
![Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (ab224677), expandable thumbnail](https://content.abcam.com/products/images/heme-oxygenase-1-antibody-epr18161-128-bsa-and-azide-free-ab224677--flow-cytometry-intracellular-img37362.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (ab224677)")
![Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (ab224677), expandable thumbnail](https://content.abcam.com/products/images/heme-oxygenase-1-antibody-epr18161-128-bsa-and-azide-free-ab224677--flow-cytometry-intracellular-img26824.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (ab224677)")
![Western blot - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (ab224677), expandable thumbnail](https://content.abcam.com/products/images/heme-oxygenase-1-antibody-epr18161-128-bsa-and-azide-free-ab224677--western-blot-img417807.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Heme Oxygenase 1 antibody [EPR18161-128] - BSA and Azide free (ab224677)")
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