Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248) is a mouse monoclonal antibody detecting Heme Oxygenase 1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ELISA. Suitable for Cow, Dog, Human, Mouse, Rat.
- Over 320 publications
- Trusted since 2004
Images
![Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (AB13248), expandable thumbnail](https://content.abcam.com/products/images/heme-oxygenase-1-antibody-ho-1-1-ab13248--western-blot-img13714.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (AB13248)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [HO-1-1] (AB13248), expandable thumbnail](https://content.abcam.com/products/images/heme-oxygenase-1-antibody-ho-1-1-ab13248--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img6502.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [HO-1-1] (AB13248)")
![Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (AB13248), expandable thumbnail](https://content.abcam.com/products/images/heme-oxygenase-1-antibody-ho-1-1-ab13248--western-blot-img128588.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (AB13248)")
![Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (AB13248), expandable thumbnail](https://content.abcam.com/products/images/heme-oxygenase-1-antibody-ho-1-1-ab13248--western-blot-img82871.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (AB13248)")
![Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (AB13248), expandable thumbnail](https://content.abcam.com/products/images/heme-oxygenase-1-antibody-ho-1-1-ab13248--western-blot-img90183.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (AB13248)")
Publications
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- Molecular neurobiology 61:2653-26712023Hyperphosphorylated Tau Inflicts Intracellular Stress Responses that Are Mitigated by Apomorphine.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenfeng Song,Kuang-Wei Wang,Hsiao-Tien Chien Hagar,Hong-Ru Chen,Chia-Yi Kuan,Kezhong Zhang,Min-Hao KuoPubMed 37919601
- PloS one 18:e02837052023Screening fructosamine-3-kinase (FN3K) inhibitors, a deglycating enzyme of oncogenic Nrf2: Human FN3K homology modelling, docking and molecular dynamics simulations.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNarasimha M Beeraka,Jin Zhang,Subhankar Mandal,Hemanth Vikram P R,Junqi Liu,Namitha B M,Di Zhao,Prashanth Vishwanath,Gurupadayya B M,Ruitai FanPubMed 37910519
- International journal of molecular sciences 24:2023Xanthones Isolated from Reduced Oxidative Stress in Periodontal Ligament Stem Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNisarat Ruangsawasdi,Nawong Boonnak,Chareerut Pruksaniyom,Pirasut RodanantPubMed 37834121
- Molecules (Basel, Switzerland) 28:2023A Standardized Botanical Composition Mitigated Acute Inflammatory Lung Injury and Reduced Mortality through Extracellular HMGB1 Reduction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMesfin Yimam,Teresa Horm,Alexandria O'Neal,Ping Jiao,Mei Hong,Lidia Brownell,Qi Jia,Mosi Lin,Alex Gauthier,Jiaqi Wu,Kranti Venkat Mateti,Xiaojian Yang,Katelyn Dial,Sidorela Zefi,Lin L MantellPubMed 37764336
- Antioxidants (Basel, Switzerland) 12:2023Copper(II) Complexes with Carnosine Conjugates of Hyaluronic Acids at Different Dipeptide Loading Percentages Behave as Multiple SOD Mimics and Stimulate Nrf2 Translocation and Antioxidant Response in In Vitro Inflammatory Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrancesco Bellia,Valeria Lanza,Irina Naletova,Barbara Tomasello,Valeria Ciaffaglione,Valentina Greco,Sebastiano Sciuto,Pietro Amico,Rosanna Inturri,Susanna Vaccaro,Tiziana Campagna,Francesco Attanasio,Giovanni Tabbì,Enrico RizzarelliPubMed 37627627
- Cell biology and toxicology 39:3031-30592023Transcriptional landscape of mitochondrial electron transport chain inhibition in renal cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGiada Carta,Wanda van der Stel,Emma W J Scuric,Liliana Capinha,Johannes Delp,Susanne Hougaard Bennekou,Anna Forsby,Paul Walker,Marcel Leist,Bob van de Water,Paul JenningsPubMed 37353587
- Antioxidants (Basel, Switzerland) 12:2023Hepatitis Delta Virus Antigens Trigger Oxidative Stress, Activate Antioxidant Nrf2/ARE Pathway, and Induce Unfolded Protein Response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOlga A Smirnova,Olga N Ivanova,Furkat Mukhtarov,Vladimir T Valuev-Elliston,Artemy P Fedulov,Petr M Rubtsov,Natalia F Zakirova,Sergey N Kochetkov,Birke Bartosch,Alexander V IvanovPubMed 37107349
- Frontiers in toxicology 5:11320202023Ethylhexadecyldimethylammonium bromide, a quaternary ammonium compound, controls inflammatory response through NRF2 pathway in a human immortalized keratinocyte cell line.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLise Aubry,Romain Vallion,Sara Salman,Marie-Hélène Damiens,Pierre-Jacques Ferret,Saadia Kerdine-RömerPubMed 37089166
- Experimental and therapeutic medicine 25:2132023Heme oxygenase‑1 inhibits renal tubular epithelial cell pyroptosis by regulating mitochondrial function through PINK1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHai-Bo Li,Yan-Shuai Mo,Xi-Zhe Zhang,Qi Zhou,Xiao-Dong Liang,Jian-Nan Song,Li-Na Hou,Jian-Nan Wu,Ying Guo,Dan-Dan Feng,Yi Sun,Jian-Bo YuPubMed 37123216
- Cancers 15:2023Zinc Finger Protein 90 Knockdown Promotes Cisplatin Sensitivity via Nrf2/HO-1 Pathway in Ovarian Cancer Cell.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChing-Hu Wu,Chien-Wei Feng,Chiu-Lin Wang,Zhi-Hong Wen,Cheng-Yu Long,Feng-Hsiang TangPubMed 36900383
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human HMOX1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P09601
Abcam Recommends
Reactivity data
Select an application| IHC-P | WB | sELISA | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Tested | Expected | Tested |
| Mouse | Expected | Expected | Tested | Expected |
| Rat | Expected | Tested | Expected | Expected |
| Cow | Expected | Tested | Expected | Expected |
| Dog | Expected | Tested | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Dog, Cow, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
Species Dog | Dilution info 4 µg/mL | Notes - |
Species Cow | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes For sandwich ELISA, use this antibody as Capture at 5 μg/ml with Rabbit polyclonal to Heme Oxygenase 1 (Anti-Heme Oxygenase 1 antibody ab13243) as Detection. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Dog, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Dog, Cow, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Heme oxygenase 1. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron (PubMed:11121422, PubMed:19556236, PubMed:7703255). Affords protection against programmed cell death and this cytoprotective effect relies on its ability to catabolize free heme and prevent it from sensitizing cells to undergo apoptosis (PubMed:20055707). Heme oxygenase 1. (Microbial infection) During SARS-COV-2 infection, promotes SARS-CoV-2 ORF3A-mediated autophagy but is unlikely to be required for ORF3A-mediated induction of reticulophagy. Heme oxygenase 1 soluble form. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron.
Alternative names
HO, HO1, HMOX1, Heme oxygenase 1, HO-1
Recommended products
Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248) is a mouse monoclonal antibody detecting Heme Oxygenase 1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ELISA. Suitable for Cow, Dog, Human, Mouse, Rat.
- Over 320 publications
- Trusted since 2004
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human HMOX1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P09601
- Clone number
- HO-1-1
- Purity
- Tissue culture supernatant
- Concentration
- Purification notes
Purified from TCS.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), ELISA in Cow, Dog, Human, Mouse, Rat samples.
What is the molecular weight of Heme Oxygenase 1?
Anti-Heme Oxygenase 1 [HO-1-1] (ab13248) specifically detects a band for Heme Oxygenase 1 (UniProt: P09601) at a molecular weight of 34.6kDa.
Trusted by the scientific community
Anti-Heme Oxygenase 1 [HO-1-1] (ab13248) was first used in a scientific publication in 2004 and has been cited over 320 times in peer-reviewed journals.
Reviewed by scientists
Anti-Heme Oxygenase 1 [HO-1-1] (ab13248) has over 15 independent reviews from customers.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Heme Oxygenase 1 also known as HO-1 or HMOX1 is an enzyme that plays an important mechanistic role in heme catabolism. It catalyzes the degradation of heme into biliverdin carbon monoxide and free iron. This process involves the cleavage of the heme ring. HO-1 has a molecular weight of approximately 32 kDa. It is widely expressed in numerous tissues but is especially abundant in the liver and spleen. Its expression is induced by heme and other stress stimuli such as heavy metals cytokines and reactive oxygen species.
- Biological function summary
Heme Oxygenase 1 serves important protective functions in the body. It is not part of a larger complex but its products such as carbon monoxide and biliverdin have their own biological activities. Carbon monoxide produced by HO-1 has antiflammatory properties and can modulate apoptotic pathways. Biliverdin is reduced to bilirubin which acts as an antioxidant. The enzyme therefore directly influences cellular stress responses and maintains cellular homeostasis through these processes.
- Pathways
Heme Oxygenase 1 is integrally involved in oxidative stress response and heme metabolism. It participates in the cellular response to oxidative damage by reducing oxidative stress and promoting cytoprotection. Through its heme degradation activity it is connected with the synthesis of biologically active molecules like bilirubin and carbon monoxide. Heme Oxygenase 1 activity is related to other proteins in oxidative stress pathways such as Nuclear Factor Erythroid 2-Related Factor 2 (Nrf2) which regulates its expression and globins which are sources of heme for HO-1 activity.
- Associated diseases and disorders
Heme Oxygenase 1 has been linked to conditions like cardiovascular diseases and neurodegenerative disorders. Its expression can attenuate the severity of atherosclerosis where oxidative stress is an important factor. In neurodegenerative diseases HO-1’s antioxidant properties may provide neuroprotection by mitigating oxidative damage. The protein's interactions with inflammatory cytokines such as Interleukin-6 and tumor necrosis factor-alpha influence its activity in these disease contexts.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
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Full details and terms and conditions can be found here:
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7 product images
![Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248), expandable thumbnail](https://content.abcam.com/products/images/heme-oxygenase-1-antibody-ho-1-1-ab13248--western-blot-img13714.jpg "Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248)")
Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248)
Hek293 & HL60 presumed negative or very low expression.
Loading control GAPDH at 38kDa
This image was generated using the ascites version of the product.
All lanes: Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248) at 1 µg/mL
Lane 1: Hek293 at 10 µg
Lane 2: HL60 at 10 µg
Lane 3: HeLa at 10 µg
Lane 4: A549 at 10 µg
Lane 5: Hu spleen at 10 µg
Lane 6: Ms spleen at 10 µg
Lane 7: Rt spleen at 10 µg
SecondaryAll lanes: IRDye® 800CW Goat anti Mouse
Predicted band size: 33 kDa
Observed band size: 32 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248), expandable thumbnail](https://content.abcam.com/products/images/heme-oxygenase-1-antibody-ho-1-1-ab13248--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img6502.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248)
IHC image of Hem Oxygensae 1 staining in a section of formalin fixed, paraffin embedded normal human spleen tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13248, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
This image was generated using the ascites version of the product.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
![Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248), expandable thumbnail](https://content.abcam.com/products/images/heme-oxygenase-1-antibody-ho-1-1-ab13248--western-blot-img128588.jpg "Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248)")
Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248)
The following proteins and lysates were electrophoresed; Lane 1 - Heme-Oxygenase-1 (Hsp32) Protein (50ng), lane 2 - Heme-Oxygenase-2 protein NSP-550 (100ng), lane 3 - MDBK Cell Lysate (20ug), lane 4 - Mouse liver microsome (20ug) and lane 5 - Dog liver microsome (20ug). ab13248 was applied at a concentration of 4ugml-1.
This image was generated using the ascites version of the product.
All lanes: Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248)
Predicted band size: 33 kDa
![Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248), expandable thumbnail](https://content.abcam.com/products/images/heme-oxygenase-1-antibody-ho-1-1-ab13248--western-blot-img82871.jpg "Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248)")
Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248)
This image was generated using the ascites version of the product.
Lane 1: MW marker
Lanes 2 - 7: Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248)
Lane 2: Recombinant Rat Heme Oxygenase 1
Lane 3: Recombinant Human Heme Oxygenase 1
Lane 4: Recombinant Human Heme Oxygenase 2
Lane 5: MDBK cell lysate
Lane 6: Dog liver microsome
Lane 7: Mouse liver microsome
Predicted band size: 33 kDa
![Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248), expandable thumbnail](https://content.abcam.com/products/images/heme-oxygenase-1-antibody-ho-1-1-ab13248--western-blot-img90183.jpg "Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248)")
Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248)
This image was generated using the ascites version of the product.
All lanes: Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248) at 1/250 dilution
All lanes: Human microsome lysate
Predicted band size: 33 kDa
![Sandwich ELISA - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248), expandable thumbnail](https://content.abcam.com/products/images/heme-oxygenase-1-antibody-ho-1-1-ab13248--sandwich-elisa-img43806.jpg "Sandwich ELISA - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248)")
Sandwich ELISA - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248)
Standard Curve for Heme Oxygenase 1 (Analyte: Heme Oxygenase 1 protein (Tagged) (Recombinant Human Heme Oxygenase 1 protein ab85243)); dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal [HO-1-1] to Heme Oxygenase 1 (ab13248) at 5μg/ml and Detector Antibody Rabbit polyclonal to Heme Oxygenase 1 (Anti-Heme Oxygenase 1 antibody ab13243) at 0.5μg/ml.
This image was generated using the ascites version of the product.
![Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248), expandable thumbnail](https://content.abcam.com/products/images/heme-oxygenase-1-antibody-ho-1-1-ab13248--flow-cytometry-intracellular-img30547.jpg "Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248)")
Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248)
ab13248 at 10μg/ml staining Heme Oxygenase 1 in human lung cancer A2 cells by flow cytometery. The left image repersents staining with isotype control antibody and the right image show staining with ab13248.
This image was generated using the ascites version of the product.
![Sandwich ELISA - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248), expandable thumbnail](https://content.abcam.com/products/images/heme-oxygenase-1-antibody-ho-1-1-ab13248--sandwich-elisa-img43806.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248)")
![Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248), expandable thumbnail](https://content.abcam.com/products/images/heme-oxygenase-1-antibody-ho-1-1-ab13248--flow-cytometry-intracellular-img30547.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248)")
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