Rabbit Polyclonal Hemogen antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HEMGN aa 100-200.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human HEMGN aa 100-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BXL5
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target data
Function
Regulates the proliferation and differentiation of hematopoietic cells. Overexpression block the TPA-induced megakaryocytic differentiation in the K562 cell model. May also prevent cell apoptosis through the activation of the nuclear factor-kappa B (NF-kB).
Alternative names
EDAG, NDR, PRO1037, PRO1620, HEMGN, Hemogen, Erythroid differentiation-associated gene protein, Hemopoietic gene protein, Negative differentiation regulator protein, EDAG-1
Recommended products
Rabbit Polyclonal Hemogen antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HEMGN aa 100-200.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human HEMGN aa 100-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BXL5
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Hemogen sometimes referred to as EDAG is a nuclear protein that plays a role in hematopoiesis. Hemogen has a molecular mass of approximately 41 kDa. The expression of Hemogen occurs primarily in the bone marrow liver and spleen. This protein is known for its regulatory functions and its involvement in cell proliferation and differentiation particularly in erythroid and myeloid lineages.
- Biological function summary
Hemogen contributes to the proliferation of blood cells by influencing gene expression. It is part of a complex interaction within the cell nucleus where it supports transcriptional regulation. Hemogen may interact with nuclear co-factors that modulate the expression of specific genes necessary for blood cell development. It also has roles in maintaining genetic stability during cell division.
- Pathways
Hemogen participates actively in hematopoietic pathways and erythropoiesis. In the hematopoietic pathway Hemogen coordinates with proteins like GATA-1 which is critical for erythroid differentiation. It also engages with signaling pathways that ensure the proper formation of red blood cells. Hemogen has been implicated in pathways governed by factors such as NF-kB where it acts to modulate immune cell function.
- Associated diseases and disorders
Hemogen has associations with conditions like leukemia and myelodysplastic syndromes. Abnormal expression or mutation of Hemogen can disrupt normal hematopoiesis leading to malignant transformations in blood cells. In leukemia cases Hemogen may exhibit interactions with proteins such as Bcl-2 influencing cell survival and apoptosis. Additionally alterations in Hemogen expression can contribute to ineffective hematopoiesis increasing the risk of blood-related disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemogen antibody (ab224417)
Paraffin-embedded human bone marrow tissue stained for Hemogen using ab224417 at 1/200 dilution in immunohistochemical analysis.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com