AB231803

Anti-Hemoglobin antibody

Name: Anti-Hemoglobin antibody (ab231803) | Abcam
Brand: Abcam Limited
SKU: AB231803

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(1 Publication)

Rabbit Polyclonal Hemoglobin subunit alpha antibody. Suitable for WB and reacts with Mouse, Cow samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Cow Hemoglobin subunit alpha aa 1 to C-terminus.

View Alternative Names

Hba-a1, Hba, Hemoglobin subunit alpha, Alpha-globin, Hemoglobin alpha chain

5 Images

Supplier Data

Western blot - Anti-Hemoglobin antibody (AB231803)

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Western blot - Anti-Hemoglobin antibody (ab231803) at 1 µg/mL

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Cow blood cell lysate

Secondary

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HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

Predicted band size: 15 kDa

false

Supplier Data

Western blot - Anti-Hemoglobin antibody (AB231803)

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Western blot - Anti-Hemoglobin antibody (ab231803) at 2 µg/mL

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Recombinant cow Hemoglobin protein

Predicted band size: 15 kDa

false

Supplier Data

Western blot - Anti-Hemoglobin antibody (AB231803)

All lanes:

Western blot - Anti-Hemoglobin antibody (ab231803) at 2 µg/mL

All lanes:

Mouse spleen lysate

Predicted band size: 15 kDa

false

Supplier Data

Western blot - Anti-Hemoglobin antibody (AB231803)

All lanes:

Western blot - Anti-Hemoglobin antibody (ab231803) at 2 µg/mL

All lanes:

Mouse liver lysate

Predicted band size: 15 kDa

false

Supplier Data

Western blot - Anti-Hemoglobin antibody (AB231803)

All lanes:

Western blot - Anti-Hemoglobin antibody (ab231803) at 2 µg/mL

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Mouse cerebrum lysate

Predicted band size: 15 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Cow

Applications

applications

Immunogen

Recombinant Fragment Protein within Cow Hemoglobin subunit alpha aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P01966

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>" }, "Goat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab231803 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hemoglobin also known as Hb or HBB is a protein found in red blood cells. It is a tetramer with a molecular mass of approximately 64.5 kDa composed of two alpha and two beta subunits. This protein is predominantly expressed in erythrocytes where it plays an important role in oxygen transport from the lungs to tissues and facilitates the return of carbon dioxide from tissues to the lungs for exhalation.

Biological function summary

Hemoglobin carries oxygen in blood and acts as a buffer in maintaining blood pH. Within the erythrocytes hemoglobin comprises one of the principal components of these cells each molecule binding up to four oxygen molecules. Hemoglobin exists as a complex with heme groups that directly interact with oxygen allowing hemoglobin to perform its respiratory function efficiently.

Pathways

Hemoglobin is important in the circulatory and respiratory pathways. It works in conjunction with proteins like myoglobin which assists with oxygen storage in muscles and cytochrome c which is important in the electron transport chain. Hemoglobin's function aligns with these pathways to maintain proper oxygen and energy levels throughout the body.

Hemoglobin abnormalities lead to conditions such as sickle cell anemia and thalassemia. Sickle cell anemia involves a mutation in the beta-globin gene resulting in deformed red blood cells that hinder oxygen delivery and cause pain due to blockages in blood vessels. Thalassemia results from reduced production of globin chains leading to anemia. These conditions involve aberrations in the hemoglobin or related proteins impacting overall health and requiring clinical management.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in oxygen transport from the lung to the various peripheral tissues.. Hemopressin. Hemopressin acts as an antagonist peptide of the cannabinoid receptor CNR1. Hemopressin-binding efficiently blocks cannabinoid receptor CNR1 and subsequent signaling.

See full target information Hba

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 28:515 PubMed39247492

2024

Analysis of metastasis‑related risk factors and clinical relevance in adult soft‑tissue sarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Han,Xin Song,Jialiang Liu,Jingfen Zhou,Zhipeng Wu,Haihan Song,Jun Tao,Jian Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Hemoglobin antibody

Western blot - Anti-Hemoglobin antibody (AB231803)

Western blot - Anti-Hemoglobin antibody (AB231803)

Western blot - Anti-Hemoglobin antibody (AB231803)

Western blot - Anti-Hemoglobin antibody (AB231803)

Western blot - Anti-Hemoglobin antibody (AB231803)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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