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Rabbit Recombinant Monoclonal Hemoglobin subunit alpha antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Cited in 31 publications.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EPR3608] (AB92492), expandable thumbnail
  • Western blot - Anti-Hemoglobin subunit alpha antibody [EPR3608] (AB92492), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EPR3608] (AB92492), expandable thumbnail
  • Western blot - Anti-Hemoglobin subunit alpha antibody [EPR3608] (AB92492), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EPR3608] (AB92492), expandable thumbnail

Publications

  • International journal of molecular sciences 24:2023
    Changes in Hemoglobin Properties in Complex with Glutathione and after Glutathionylation.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Iuliia D Kuleshova,Pavel I Zaripov,Yuri M Poluektov,Anastasia A Anashkina,Dmitry N Kaluzhny,Evgeniia Yu Parshina,Georgy V Maksimov,Vladimir A Mitkevich,Alexander A Makarov,Irina Yu Petrushanko
    PubMed 37686361
  • Biomedicines 11:2023
    Long-Term L-Glutamine Treatment Reduces Hemolysis without Ameliorating Hepatic Vaso-Occlusion and Liver Fibrosis in a Mouse Model of Sickle Cell Disease.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Omika Katoch,Ramakrishna Ungalara,Tomasz Kaminski,Ziming Li,Rikesh K Dubey,Isabella Burholt,Shweta Gudapati,Tirthadipa Pradhan-Sundd
    PubMed 37760853

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWB
Human
Tested
Not recommended
Not recommended
Tested
Mouse
Tested
Not recommended
Not recommended
Tested
Rat
Tested
Not recommended
Not recommended
Tested

Tested
Tested

Species

Mouse

Dilution info

1/100 - 1/500

Notes

Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/100 - 1/500

Notes

Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Species

Human

Dilution info

1/100 - 1/500

Notes

Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species

Rat

Dilution info

-

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species

Human

Dilution info

-

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species

Mouse

Dilution info

1/1000 - 1/2000

Notes

-

Species

Rat

Dilution info

1/1000 - 1/2000

Notes

-

Species

Human

Dilution info

1/1000 - 1/2000

Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

    5 products for Alternative Version

      Target data

      Function

      Involved in oxygen transport from the lung to the various peripheral tissues.

      Alternative names

      Hemoglobin subunit alpha, Alpha-globin, Hemoglobin alpha chain, HBA2, HBA1

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      Rabbit Recombinant Monoclonal Hemoglobin subunit alpha antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Cited in 31 publications.

      Alternative names

      Hemoglobin subunit alpha, Alpha-globin, Hemoglobin alpha chain, HBA2, HBA1

      Key facts

      Isotype

      IgG

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Clone number

      EPR3608

      Purification technique

      Affinity purification Protein A

      Specificity

      This antibody gave a positive signal in Western blotting of rat brain, heart, kidney and spleen tissue. This antibody works in human, mouse and rat in IHC.

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate short-term storage duration

      1-2 weeks

      Appropriate short-term storage conditions

      +4°C

      Appropriate long-term storage conditions

      -20°C

      Aliquoting information

      Upon delivery aliquot

      Storage information

      Avoid freeze / thaw cycle

      Notes

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      Supplementary info

      Activity summary

      Hemoglobin subunit alpha also known as alpha-globin is a component of the hemoglobin protein complex which plays an important role in oxygen transport within the blood. Alpha-globin has an approximate molecular weight of 15.1 kDa and is expressed highly in the red blood cells. It is part of the hemoglobin tetramer along with two beta subunits each one containing an iron-bound heme group. Variants of the alpha chain can be studied using hemoglobin peptides or denatured hemoglobin samples. Researchers can further analyze the alpha hemoglobin using methods like SDS-PAGE or alpha ELISA assays.

      Biological function summary

      Hemoglobin subunit alpha forms an important part of the hemoglobin complex facilitating the binding and release of oxygen molecules as blood circulates in the body. Alpha hemoglobin ensures efficient loading of oxygen in the lungs and unloading in tissues maintaining cellular respiration. The subunit plays a structural role as well stabilizing the hemoglobin tetramer for optimal function. Its ability to carry oxygen depends on the cooperative interaction between its alpha and beta globin counterparts.

      Pathways

      Hemoglobin subunit alpha operates predominantly within the oxygen transport pathway which is essential to meet the metabolic demands of cells. It also links to pathways involving iron metabolism given its coordination with heme groups. Alpha hemoglobin interacts cooperatively with proteins such as beta-globin to ensure efficient oxygen delivery. This interplay is highlighted when examining hemoglobin biosynthesis and breakdown pathways.

      Associated diseases and disorders

      Alpha-globin is linked to conditions like alpha-thalassemia and sickle cell disease. These disorders result from mutations in the hemoglobin alpha or beta subunits leading to imbalanced globin production or abnormal hemoglobin structures. Alpha thalassemia is connected with unequal production of globin chains affecting hemoglobin stability while beta-globin mutations lead to sickle cell disease with altered oxygen delivery. Anti-hemoglobin antibodies might help in researching these conditions allowing a better understanding of molecular changes and potential therapeutic targets.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      9 product images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labeling Hemoglobin subunit alpha antibody with purified ab92492 at 1/500 dilution (1.4 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody at 1/500 dilution. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

      • Western blot - Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492), expandable thumbnail

        Western blot - Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492)

        Blocking and diluting buffer: 5% NFDM/TBST.

        All lanes: Western blot - Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492) at 1/2000 dilution

        Lane 1: Human heart lysate at 15 µg

        Lane 2: Rat heart lysate at 15 µg

        Secondary

        All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

        Predicted band size: 15 kDa

        Observed band size: 14 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebral cortex tissue labeling Hemoglobin subunit alpha antibody with purified ab92492 at 1/500 dilution (1.4 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody at 1/500 dilution. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

      • Western blot - Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492), expandable thumbnail

        Western blot - Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492)

        Blocking and diluting buffer and concentration: 5% NFDM /TBST.

        All lanes: Western blot - Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492) at 1/10000 dilution

        All lanes: Mouse spleen lysate at 15 µg

        Secondary

        All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

        Predicted band size: 15 kDa

        Observed band size: 14 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492)

        Immunohistochemical analysis of paraffin embedded human placenta tissue labeling hemoglobin subunit alpha with unpurified ab92492 at 0.051 μg/ml (1:40000 dilution).

        Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

      • Western blot - Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492), expandable thumbnail

        Western blot - Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492)

        All lanes: Western blot - Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492) at 1/1000 dilution

        Lane 1: K562 cell lysate at 10 µg

        Lane 2: Human heart lysate at 10 µg

        Secondary

        All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 15 kDa

        Observed band size: 14 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492)

        Immunohistochemical analysis of paraffin embedded human placenta tissue labeling hemoglobin subunit alpha with unpurified ab92492 at 0.051 μg/ml (1:40000 dilution).

        Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492)

        Immunohistochemical analysis of paraffin embedded human stomach cancer tissue labeling hemoglobin subunit alpha with unpurified ab92492 at 0.051 μg/ml (1:40000 dilution).

        Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labeling Hemoglobin subunit alpha antibody with purified ab92492 at 1/500 dilution (1.4 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody at 1/500 dilution. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

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      For this product, it's our understanding that no specific protocols are required. You can:

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