Anti-Hemoglobin subunit alpha antibody [EPR3608] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EPR3608] - BSA and Azide free (AB174536)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labeling Hemoglobin subunit alpha antibody with purified ab92492 at 1/500 dilution (1.4 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. ab97051, a HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody at 1/500 dilution. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92492).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EPR3608] - BSA and Azide free (AB174536)

This IHC data was generated using the same anti-hemoglobin subunit alpha antibody clone, EPR3608, in a different buffer formulation (cat# ab92492).

Immunohistochemical analysis of paraffin embedded human stomach cancer tissue labeling hemoglobin subunit alpha with unpurified ab92492 at 0.051 μg/ml (1 : 40000 dilution).

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EPR3608] - BSA and Azide free (AB174536)

Immunohistochemical analysis of paraffin embedded human placenta tissue labeling hemoglobin subunit alpha with unpurified ab92492 at 0.051 μg/ml (1 : 40000 dilution).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92492).

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EPR3608] - BSA and Azide free (AB174536)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebral cortex tissue labeling Hemoglobin subunit alpha antibody with purified ab92492 at 1/500 dilution (1.4 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. ab97051, a HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody at 1/500 dilution. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92492).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EPR3608] - BSA and Azide free (AB174536)

Immunohistochemical analysis of paraffin embedded human placenta tissue labeling hemoglobin subunit alpha with unpurified ab92492 at 0.051 μg/ml (1 : 40000 dilution).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92492).

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EPR3608] - BSA and Azide free (AB174536)

This IHC data was generated using the same anti-hemoglobin subunit alpha antibody clone, EPR3608, in a different buffer formulation (cat# ab92492).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labeling Hemoglobin subunit alpha antibody with purified ab92492 at 1/500 dilution (1.4 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. ab97051, a HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody at 1/500 dilution. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EPR3608] - BSA and Azide free (AB174536)

Clone EPR3608 (ab174536) has been successfully conjugated by Abcam. This image was generated using Anti-Hemoglobin subunit alpha antibody [EPR3608] (Alexa Fluor® 647). Please refer to ab215919 for protocol details.

IHC image of Hemoglobin subunit alpha staining in a section of formalin-fixed paraffin-embedded normal human placenta*.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Dako Pascal pressure cooker using the standard factory-set regime. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature.  The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab215919 at 1/100 dilution (shown in red) and counterstained using ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.