Rabbit Recombinant Monoclonal HBB antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 5 publications.
Images
![Immunoprecipitation - Anti-Hemoglobin subunit beta antibody [EPR20614] (AB214049), expandable thumbnail](https://content.abcam.com/products/images/hemoglobin-subunit-beta-antibody-epr20614-ab214049--immunoprecipitation-img5122.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Hemoglobin subunit beta antibody [EPR20614] (AB214049)")
![Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (AB214049), expandable thumbnail](https://content.abcam.com/products/images/hemoglobin-subunit-beta-antibody-epr20614-ab214049--western-blot-img164721.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (AB214049)")
![Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (AB214049), expandable thumbnail](https://content.abcam.com/products/images/hemoglobin-subunit-beta-antibody-epr20614-ab214049--western-blot-img148334.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (AB214049)")
![Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (AB214049), expandable thumbnail](https://content.abcam.com/products/images/hemoglobin-subunit-beta-antibody-epr20614-ab214049--western-blot-img151892.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (AB214049)")
![Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (AB214049), expandable thumbnail](https://content.abcam.com/products/images/hemoglobin-subunit-beta-antibody-epr20614-ab214049--western-blot-img139780.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (AB214049)")
Publications
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- Redox biology 58:1025352022Hemoglobin is an oxygen-dependent glutathione buffer adapting the intracellular reduced glutathione levels to oxygen availability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSimone Fenk,Elizaveta V Melnikova,Anastasia A Anashkina,Yuri M Poluektov,Pavel I Zaripov,Vladimir A Mitkevich,Yaroslav V Tkachev,Lars Kaestner,Giampaolo Minetti,Heimo Mairbäurl,Jeroen S Goede,Alexander A Makarov,Irina Yu Petrushanko,Anna BogdanovaPubMed 36413919
- Heliyon 8:e112712022First-in-human autologous implantation of genetically modified adipocytes expressing LCAT for the treatment of familial LCAT deficiency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMasayuki Aso,Tokuo T Yamamoto,Masayuki Kuroda,Jun Wada,Yoshitaka Kubota,Ko Ishikawa,Yoshiro Maezawa,Naoya Teramoto,Ayako Tawada,Sakiyo Asada,Yasuyuki Aoyagi,Mika Kirinashizawa,Akinobu Onitake,Yuta Matsuura,Kunio Yasunaga,Shun-Ichi Konno,Katsuaki Nishino,Misato Yamamoto,Junko Miyoshi,Norihiko Kobayashi,Masami Tanio,Takayuki Ikeuchi,Hidetoshi Igari,Nobuyuki Mitsukawa,Hideki Hanaoka,Koutaro Yokote,Yasushi SaitoPubMed 36387451
- Journal of immunology research 2022:84404222022Transmembrane Protein ANTXR1 Regulates -Globin Expression by Targeting the Wnt/-Catenin Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTingting Jin,Zhaojun Zhang,Yuanyuan Han,Di Li,Juan Liu,Minmin Jiang,Junwei Zhu,Ryo Kurita,Yukio Nakamura,Fangfang Hu,Yongjie Xu,Xiangdong Fang,Shengwen Huang,Zhaolin SunPubMed 35942209
- Frontiers in oncology 11:6813662021N-Myristoylation by NMT1 Is POTEE-Dependent to Stimulate Liver Tumorigenesis Differentially Regulating Ubiquitination of Targets.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuoqing Zhu,Feng Wang,Haojie Li,Xiao Zhang,Qi Wu,Ya Liu,Mingping Qian,Susu Guo,Yueyue Yang,Xiangfei Xue,Fenyong Sun,Yongxia Qiao,Qiuhui PanPubMed 34136404
- Science advances 6:2020Supramolecular nanosubstrate-mediated delivery system enables CRISPR-Cas9 knockin of hemoglobin beta gene for hemoglobinopathies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeng Yang et. al.PubMed 33097539
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Expected | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/40 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Involved in oxygen transport from the lung to the various peripheral tissues. LVV-hemorphin-7 potentiates the activity of bradykinin, causing a decrease in blood pressure. Spinorphin. Functions as an endogenous inhibitor of enkephalin-degrading enzymes such as DPP3, and as a selective antagonist of the P2RX3 receptor which is involved in pain signaling, these properties implicate it as a regulator of pain and inflammation.
Alternative names
Hemoglobin subunit beta, Beta-globin, Hemoglobin beta chain, HBB
Recommended products
Rabbit Recombinant Monoclonal HBB antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 5 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR20614
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The hemoglobin subunit beta also known as beta hemoglobin or hemoglobin beta subunit is a component of the larger hemoglobin molecule important for transporting oxygen in red blood cells. This protein has a molecular weight of approximately 16 kDa. Hemoglobin subunit beta joins with alpha hemoglobin subunit to form hemoglobin A the most common form in adult humans. Expression of this protein happens in the erythroid cells of the bone marrow where it plays an important role in the production of hemoglobin during red blood cell formation.
- Biological function summary
The hemoglobin beta subunit is essential for binding and releasing oxygen to tissues throughout the body. As part of the hemoglobin complex it helps stabilize the oxygen binding affinity through cooperative interactions with the alpha subunits. When oxygen binds to the heme groups in these subunits a conformational change occurs enhancing the efficiency of oxygen transport. This dynamic action allows hemoglobin to carry oxygen from the lungs to tissues and return carbon dioxide for expulsion.
- Pathways
Hemoglobin beta subunit significantly participates in the oxygen transport and carbon dioxide exchange pathways. It interacts closely with carbonic anhydrase in the process of carbon dioxide transport converting it to bicarbonate for efficient clearance from the body. In this capacity the protein stands as a central component of the erythrocyte function and blood gas transport system partnering physiologically with proteins such as cytochrome b5 reductase in electron transfer-related pathways.
- Associated diseases and disorders
Hemoglobin subunit beta is prominently associated with hemoglobinopathies like sickle cell disease and beta-thalassemia. Mutations in its gene can lead to abnormal hemoglobin function or structure causing red blood cell disorders that impact oxygen delivery. In sickle cell disease an amino acid substitution causes hemoglobin to polymerize under low oxygen resulting in sickle-shaped cells. In beta-thalassemia reduced or absent beta-globin chain production leads to anemia. These hemoglobin-related disorders are tightly connected to genetic mutations influencing other proteins such as alpha-globin complicating the clinical picture.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunoprecipitation - Anti-Hemoglobin subunit beta antibody [EPR20614] (ab214049), expandable thumbnail](https://content.abcam.com/products/images/hemoglobin-subunit-beta-antibody-epr20614-ab214049--immunoprecipitation-img5122.jpg "Immunoprecipitation - Anti-Hemoglobin subunit beta antibody [EPR20614] (ab214049)")
Immunoprecipitation - Anti-Hemoglobin subunit beta antibody [EPR20614] (ab214049)
Hemoglobin subunit beta/ba1 was immunoprecipitated from 0.35 mg of TF-1 (human bone marrow erythroleukemia cell line) lysate with ab214049 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab214049 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution
Lane 1: TF-1 whole cell lysate 10 μg (Input).
Lane 2: ab214049 IP in TF-1 whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab214049 in TF-1 whole cell lysate (-).
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
All lanes: Immunoprecipitation - Anti-Hemoglobin subunit beta antibody [EPR20614] (ab214049)
Predicted band size: 16 kDa
Observed band size: 13 kDa
![Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (ab214049), expandable thumbnail](https://content.abcam.com/products/images/hemoglobin-subunit-beta-antibody-epr20614-ab214049--western-blot-img164721.jpg "Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (ab214049)")
Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (ab214049)
Blocking/Dilution buffer: 5% NFDM/TBST.All lanes: Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (ab214049) at 1/1000 dilution
Lane 1: Western blot - Recombinant Human Hemoglobin subunit alpha protein (denatured) (Recombinant Human Hemoglobin subunit alpha protein (denatured) ab131697) at 0.01 µg
Lane 2: His-tagged human Hemoglobin subunit beta/ba1 (aa3-147) recombinant protein at 0.01 µg
Lane 3: His-tagged human Hemoglobin subunit delta (aa3-147) recombinant protein at 0.01 µg
Lane 4: His-tagged human Hemoglobin subunit epsilon (aa3-147) recombinant protein at 0.01 µg
Lane 5: His-tagged human Hemoglobin subunit Gamma-2 (aa2-147) recombinant protein at 0.01 µg
Lane 6: His-tagged human Hemoglobin subunit Gamma-1 (aa2-147) recombinant protein at 0.01 µg
Lane 7: Western blot - Recombinant Human Hemoglobin subunit zeta protein (Recombinant Human Hemoglobin subunit zeta protein ab95347) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 16 kDa
Observed band size: 15 kDa
Exposure time: 3s
![Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (ab214049), expandable thumbnail](https://content.abcam.com/products/images/hemoglobin-subunit-beta-antibody-epr20614-ab214049--western-blot-img148334.jpg "Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (ab214049)")
Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (ab214049)
Blocking/Dilution buffer: 5% NFDM/TBST.Positive Control: Anti-GAPDHantibody Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (below)
It has been shown that hemoglobin subunit beta/ba1 is not expressed in HEK-293 cells.
All lanes: Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (ab214049) at 1/1000 dilution
Lane 1: TF-1 (human bone marrow erythroleukemia cell line) whole cell lysate at 10 µg
Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 16 kDa
Observed band size: 13 kDa
Exposure time: 3min
![Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (ab214049), expandable thumbnail](https://content.abcam.com/products/images/hemoglobin-subunit-beta-antibody-epr20614-ab214049--western-blot-img151892.jpg "Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (ab214049)")
Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (ab214049)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (ab214049) at 1/10000 dilution
Lane 1: Human fetal heart lysate at 10 µg
Lane 2: Human lung lysate at 20 µg
SecondaryAll lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/4000 dilution
Developed using the ECL technique.
Predicted band size: 16 kDa
Observed band size: 13 kDa
Exposure time: 3min
![Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (ab214049), expandable thumbnail](https://content.abcam.com/products/images/hemoglobin-subunit-beta-antibody-epr20614-ab214049--western-blot-img139780.jpg "Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (ab214049)")
Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (ab214049)
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1-2: 3 minutes; Lane 3: 10 seconds.
Lanes 1 and 3: Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (ab214049) at 1/1000 dilution
Lane 2: Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (ab214049) at 1/10000 dilution
Lane 1: Rat heart lysate at 20 µg
Lane 2: Mouse heart lysate at 20 µg
Lane 3: Mouse lung lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 16 kDa
Observed band size: 13 kDa
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