AB214049

Anti-Hemoglobin subunit beta antibody [EPR20614]

20ul selling size
RabMAb
Recombinant
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(7 Publications)

Rabbit Recombinant Monoclonal HBB antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 7 publications.

View Alternative Names

Hemoglobin subunit beta, Beta-globin, Hemoglobin beta chain, HBB

5 Images

Supplier Data

Immunoprecipitation - Anti-Hemoglobin subunit beta antibody [EPR20614] (AB214049)

Hemoglobin subunit beta/ba1 was immunoprecipitated from 0.35 mg of TF-1 (human bone marrow erythroleukemia cell line) lysate with ab214049 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab214049 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

Lane 1 : TF-1 whole cell lysate 10 μg (Input).

Lane 2 : ab214049 IP in TF-1 whole cell lysate (+).

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab214049 in TF-1 whole cell lysate (-).

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-Hemoglobin subunit beta antibody [EPR20614] (ab214049)

Predicted band size: 16 kDa

Observed band size: 13 kDa

false

Supplier Data

Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (AB214049)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (ab214049) at 1/10000 dilution

Lane 1:

Human fetal heart lysate at 10 µg

Lane 2:

Human lung lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/4000 dilution

Predicted band size: 16 kDa

Observed band size: 13 kDa

true

Exposure time: 3min

Supplier Data

Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (AB214049)

Blocking/Dilution buffer : 5% NFDM/TBST.

Positive Control : Anti-GAPDH antibody ab181602 (below)

It has been shown that hemoglobin subunit beta/ba1 is not expressed in HEK-293 cells.

All lanes:

Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (ab214049) at 1/1000 dilution

Lane 1:

TF-1 (human bone marrow erythroleukemia cell line) whole cell lysate at 10 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 16 kDa

Observed band size: 13 kDa

true

Exposure time: 3min

Supplier Data

Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (AB214049)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1-2 : 3 minutes; Lane 3 : 10 seconds.

Lanes 1 and 3:

Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (ab214049) at 1/1000 dilution

Lane 2:

Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (ab214049) at 1/10000 dilution

Lane 1:

Rat heart lysate at 20 µg

Lane 2:

Mouse heart lysate at 20 µg

Lane 3:

Mouse lung lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 16 kDa

Observed band size: 13 kDa

true

Lab

Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (AB214049)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (ab214049) at 1/1000 dilution

Lane 1:

Western blot - Recombinant Human Hemoglobin subunit alpha protein (denatured) (DDDDK tag N-Term + His tag N-Term) (<a href='/en-us/products/proteins-peptides/recombinant-human-hemoglobin-subunit-alpha-protein-denatured-ab131697'>ab131697</a>) at 0.01 µg

Lane 2:

His-tagged human Hemoglobin subunit beta/ba1 (aa3-147) recombinant protein at 0.01 µg

Lane 3:

His-tagged human Hemoglobin subunit delta (aa3-147) recombinant protein at 0.01 µg

Lane 4:

His-tagged human Hemoglobin subunit epsilon (aa3-147) recombinant protein at 0.01 µg

Lane 5:

His-tagged human Hemoglobin subunit Gamma-2 (aa2-147) recombinant protein at 0.01 µg

Lane 6:

His-tagged human Hemoglobin subunit Gamma-1 (aa2-147) recombinant protein at 0.01 µg

Lane 7:

Western blot - Recombinant Human Hemoglobin subunit zeta protein (His tag C-Terminus) (<a href='/en-us/products/proteins-peptides/recombinant-human-hemoglobin-subunit-zeta-protein-ab95347'>ab95347</a>) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 16 kDa

Observed band size: 15 kDa

true

Exposure time: 3s

Carrier free

Anti-Hemoglobin subunit beta antibody [EPR20614] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20614

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The hemoglobin subunit beta also known as beta hemoglobin or hemoglobin beta subunit is a component of the larger hemoglobin molecule important for transporting oxygen in red blood cells. This protein has a molecular weight of approximately 16 kDa. Hemoglobin subunit beta joins with alpha hemoglobin subunit to form hemoglobin A the most common form in adult humans. Expression of this protein happens in the erythroid cells of the bone marrow where it plays an important role in the production of hemoglobin during red blood cell formation.

Biological function summary

The hemoglobin beta subunit is essential for binding and releasing oxygen to tissues throughout the body. As part of the hemoglobin complex it helps stabilize the oxygen binding affinity through cooperative interactions with the alpha subunits. When oxygen binds to the heme groups in these subunits a conformational change occurs enhancing the efficiency of oxygen transport. This dynamic action allows hemoglobin to carry oxygen from the lungs to tissues and return carbon dioxide for expulsion.

Pathways

Hemoglobin beta subunit significantly participates in the oxygen transport and carbon dioxide exchange pathways. It interacts closely with carbonic anhydrase in the process of carbon dioxide transport converting it to bicarbonate for efficient clearance from the body. In this capacity the protein stands as a central component of the erythrocyte function and blood gas transport system partnering physiologically with proteins such as cytochrome b5 reductase in electron transfer-related pathways.

Hemoglobin subunit beta is prominently associated with hemoglobinopathies like sickle cell disease and beta-thalassemia. Mutations in its gene can lead to abnormal hemoglobin function or structure causing red blood cell disorders that impact oxygen delivery. In sickle cell disease an amino acid substitution causes hemoglobin to polymerize under low oxygen resulting in sickle-shaped cells. In beta-thalassemia reduced or absent beta-globin chain production leads to anemia. These hemoglobin-related disorders are tightly connected to genetic mutations influencing other proteins such as alpha-globin complicating the clinical picture.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in oxygen transport from the lung to the various peripheral tissues.. LVV-hemorphin-7 potentiates the activity of bradykinin, causing a decrease in blood pressure.. Spinorphin. Functions as an endogenous inhibitor of enkephalin-degrading enzymes such as DPP3, and as a selective antagonist of the P2RX3 receptor which is involved in pain signaling, these properties implicate it as a regulator of pain and inflammation.

See full target information HBB

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Signal transduction and targeted therapy 10:270 PubMed40846833

2025

Hemoglobin as a pseudoperoxidase and drug target for oxidative stress-related diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Woojin Won,Elijah Hwejin Lee,Lizaveta Gotina,Heejung Chun,Jae-Hun Lee,Mridula Bhalla,Uiyeol Park,Daeun Kim,Tai Young Kim,Ji Won Choi,Yoowon Kim,Sun Jun Park,Jiwoon Lim,Jong-Hyun Park,Hyeon Jeong Kim,Jun Young Heo,Woosuk Chung,Myung Jin Oh,Hyun Joo An,Junghee Lee,Soo-Jin Oh,Hoon Ryu,Ae Nim Pae,Ki Duk Park,C Justin Lee

Cancer cell international 24:71 PubMed38347631

2024

YY1: a key regulator inhibits gastric cancer ferroptosis and mediating apatinib-resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Zi-Han Geng,Jun-Xian Du,Yue-Da Chen,Pei-Yao Fu,Ping-Hong Zhou,Wen-Zheng Qin,Yi-Hong Luo

Redox biology 58:102535 PubMed36413919

2022

Hemoglobin is an oxygen-dependent glutathione buffer adapting the intracellular reduced glutathione levels to oxygen availability.

Applications

Unspecified application

Species

Unspecified reactive species

Simone Fenk,Elizaveta V Melnikova,Anastasia A Anashkina,Yuri M Poluektov,Pavel I Zaripov,Vladimir A Mitkevich,Yaroslav V Tkachev,Lars Kaestner,Giampaolo Minetti,Heimo Mairbäurl,Jeroen S Goede,Alexander A Makarov,Irina Yu Petrushanko,Anna Bogdanova

Heliyon 8:e11271 PubMed36387451

2022

First-in-human autologous implantation of genetically modified adipocytes expressing LCAT for the treatment of familial LCAT deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Masayuki Aso,Tokuo T Yamamoto,Masayuki Kuroda,Jun Wada,Yoshitaka Kubota,Ko Ishikawa,Yoshiro Maezawa,Naoya Teramoto,Ayako Tawada,Sakiyo Asada,Yasuyuki Aoyagi,Mika Kirinashizawa,Akinobu Onitake,Yuta Matsuura,Kunio Yasunaga,Shun-Ichi Konno,Katsuaki Nishino,Misato Yamamoto,Junko Miyoshi,Norihiko Kobayashi,Masami Tanio,Takayuki Ikeuchi,Hidetoshi Igari,Nobuyuki Mitsukawa,Hideki Hanaoka,Koutaro Yokote,Yasushi Saito

Journal of immunology research 2022:8440422 PubMed35942209

2022

Transmembrane Protein ANTXR1 Regulates -Globin Expression by Targeting the Wnt/-Catenin Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Jin,Zhaojun Zhang,Yuanyuan Han,Di Li,Juan Liu,Minmin Jiang,Junwei Zhu,Ryo Kurita,Yukio Nakamura,Fangfang Hu,Yongjie Xu,Xiangdong Fang,Shengwen Huang,Zhaolin Sun

Frontiers in oncology 11:681366 PubMed34136404

2021

N-Myristoylation by NMT1 Is POTEE-Dependent to Stimulate Liver Tumorigenesis Differentially Regulating Ubiquitination of Targets.

Applications

Unspecified application

Species

Unspecified reactive species

Guoqing Zhu,Feng Wang,Haojie Li,Xiao Zhang,Qi Wu,Ya Liu,Mingping Qian,Susu Guo,Yueyue Yang,Xiangfei Xue,Fenyong Sun,Yongxia Qiao,Qiuhui Pan

Science advances 6: PubMed33097539

2020

Supramolecular nanosubstrate-mediated delivery system enables CRISPR-Cas9 knockin of hemoglobin beta gene for hemoglobinopathies.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Yang,Shih-Jie Chou,Jindian Li,Wenqiao Hui,Wenfei Liu,Na Sun,Ryan Y Zhang,Yazhen Zhu,Ming-Long Tsai,Henkie I Lai,Matthew Smalley,Xinyue Zhang,Jiayuan Chen,Zulema Romero,Dahai Liu,Zunfu Ke,Chang Zou,Chin-Fa Lee,Steven J Jonas,Qian Ban,Paul S Weiss,Donald B Kohn,Kai Chen,Shih-Hwa Chiou,Hsian-Rong Tseng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Hemoglobin subunit beta antibody [EPR20614]

Immunoprecipitation - Anti-Hemoglobin subunit beta antibody [EPR20614] (AB214049)

Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (AB214049)

Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (AB214049)

Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (AB214049)

Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (AB214049)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

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Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise