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Rabbit Recombinant Monoclonal Hemoglobin subunit beta/ba1 antibody. Suitable for IHC-P, WB and reacts with Human, Recombinant fragment - Human samples. Cited in 1 publication.

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Images

Western blot - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (AB131225), expandable thumbnail
  • Western blot - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (AB131225), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (AB131225), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (AB131225), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBIP
Human
Tested
Tested
Not recommended
Recombinant fragment - Human
Not recommended
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1/250 - 1/500
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/200 - 1/10000
Notes

-

Species
Recombinant fragment - Human
Dilution info
1/200 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Human, Recombinant fragment - Human
Dilution info
-
Notes

-

Target data

Function

Involved in oxygen transport from the lung to the various peripheral tissues.

Additional Targets

HBB

Alternative names

Hemoglobin subunit delta, Delta-globin, Hemoglobin delta chain, HBD

Recommended products

Rabbit Recombinant Monoclonal Hemoglobin subunit beta/ba1 antibody. Suitable for IHC-P, WB and reacts with Human, Recombinant fragment - Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR8322(B)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Hemoglobin subunit delta also known as Hb delta or delta hemoglobin is a component of the hemoglobin protein family which primarily includes hemoglobin subunit beta (Hb beta). Hemoglobin delta has a molecular mass of approximately 16 kDa. It expresses mainly in erythroid cells where it functions as part of the hemoglobin A2 (HbA2) molecule. HbA2 is a minor hemoglobin variant which includes two alpha globin chains and two delta globin chains. Delta hemoglobin plays a role in oxygen transport in red blood cells albeit to a lesser extent than the major form hemoglobin A (HbA) which contains hemoglobin subunit beta.

Biological function summary

Hemoglobin subunit delta participates in oxygen binding and transport. As part of the hemoglobin A2 complex it facilitates the pickup and release of oxygen molecules in tissues and organs. Although HbA2 only constitutes about 2-3% of total hemoglobin in adults it serves as an important backup system for effective oxygen delivery. The delta subunit closely interacts with partners like the alpha globin chains to perform its role in the hemoglobin complex.

Pathways

Hemoglobin subunit delta participates in the erythropoiesis pathway which is critical for red blood cell development. It works alongside proteins such as adult hemoglobin forms and alpha-like globins to ensure proper tissue oxygenation. The hypoxia-inducible factor pathway also involves hemoglobin components adjusting erythropoiesis in response to low oxygen levels though the direct role of delta hemoglobin in this adaptive process is comparatively minor.

Associated diseases and disorders

Hemoglobin subunit delta has a connection with beta-thalassemia. This disorder results from mutations affecting beta globin synthesis leading to insufficient hemoglobin function. As a result HbA2 levels often rise as a compensatory mechanism. Sickle cell anemia mainly linked to mutations in the beta globin gene also exhibits some compensatory shifts in globin expression although delta hemoglobin itself is not directly involved. These disorders highlight the interconnected role of different hemoglobin chains including delta hemoglobin in red blood cell pathophysiology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225), expandable thumbnail

    Western blot - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)

    Blocking/Dilutiing buffer and concentration: 5% NFDM/TBST

    Exposure time: 100 seconds

    All lanes: Western blot - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225) at 1/200 dilution

    Lane 1: Western blot - Recombinant Human Hemoglobin subunit alpha protein (denatured) (Recombinant Human Hemoglobin subunit alpha protein (denatured) ab131697) at 0.1 µg

    Lane 2: His-tagged human Hemoglobin subunit beta/ba1 (aa 3-147) recombinant protein at 0.1 µg

    Lane 3: His-tagged human Hemoglobin subunit delta (aa 3-147) recombinant protein at 0.1 µg

    Lane 4: His-tagged full length human Hemoglobin subunit delta recombinant protein at 0.1 µg

    Lane 5: His-tagged human Hemoglobin subunit epsilon (aa 3-147) recombinant protein at 0.1 µg

    Lane 6: His-tagged human Hemoglobin subunit gamma-2 (aa 2-147) recombinant protein at 0.1 µg

    Lane 7: His-tagged human Hemoglobin subunit gamma-1 (aa 2-147) recombinant protein at 0.1 µg

    Lane 8: Western blot - Recombinant Human Hemoglobin subunit zeta protein (Recombinant Human Hemoglobin subunit zeta protein ab95347) at 0.1 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  • Western blot - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225), expandable thumbnail

    Western blot - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)

    All lanes: Western blot - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225) at 1/1000 dilution

    Lane 1: Human fetal heart tissue lysate at 10 µg

    Lane 2: Human fetal liver tissue lysate at 10 µg

    Lane 3: Human red blood cell (RBC) lysate at 10 µg

    Secondary

    All lanes: Goat anti-Rabbit HRP at 1/2000 dilution

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)

    Immunohistochemical analysis of paraffin embedded Human endometrium tissue labelling Hemoglobin subunit delta with ab131225 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)

    Immunohistochemical analysis of paraffin embedded Human spleen tissue labelling Hemoglobin subunit delta with ab131225 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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