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AB131225

Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)]

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(2 Publications)

Rabbit Recombinant Monoclonal Hemoglobin subunit beta/ba1 antibody. Suitable for IHC-P, WB and reacts with Human, Recombinant fragment - Human samples. Cited in 2 publications.

View Alternative Names

Hemoglobin subunit delta, Delta-globin, Hemoglobin delta chain, HBD

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (AB131225)
  • IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (AB131225)

Immunohistochemical analysis of paraffin embedded Human endometrium tissue labelling Hemoglobin subunit delta with ab131225 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (AB131225)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (AB131225)

Immunohistochemical analysis of paraffin embedded Human spleen tissue labelling Hemoglobin subunit delta with ab131225 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (AB131225)
  • WB

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Western blot - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (AB131225)

All lanes:

Western blot - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225) at 1/1000 dilution

Lane 1:

Human fetal heart tissue lysate at 10 µg

Lane 2:

Human fetal liver tissue lysate at 10 µg

Lane 3:

Human red blood cell (RBC) lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

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Western blot - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (AB131225)
  • WB

Supplier Data

Western blot - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (AB131225)

Blocking/Dilutiing buffer and concentration : 5% NFDM/TBST

Exposure time : 100 seconds

All lanes:

Western blot - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225) at 1/200 dilution

Lane 1:

Western blot - Recombinant Human Hemoglobin subunit alpha protein (denatured) (DDDDK tag N-Term + His tag N-Term) (<a href='/en-us/products/proteins-peptides/recombinant-human-hemoglobin-subunit-alpha-protein-denatured-ab131697'>ab131697</a>) at 0.1 µg

Lane 2:

His-tagged human Hemoglobin subunit beta/ba1 (aa 3-147) recombinant protein at 0.1 µg

Lane 3:

His-tagged human Hemoglobin subunit delta (aa 3-147) recombinant protein at 0.1 µg

Lane 4:

His-tagged full length human Hemoglobin subunit delta recombinant protein at 0.1 µg

Lane 5:

His-tagged human Hemoglobin subunit epsilon (aa 3-147) recombinant protein at 0.1 µg

Lane 6:

His-tagged human Hemoglobin subunit gamma-2 (aa 2-147) recombinant protein at 0.1 µg

Lane 7:

His-tagged human Hemoglobin subunit gamma-1 (aa 2-147) recombinant protein at 0.1 µg

Lane 8:

Western blot - Recombinant Human Hemoglobin subunit zeta protein (His tag C-Terminus) (<a href='/en-us/products/proteins-peptides/recombinant-human-hemoglobin-subunit-zeta-protein-ab95347'>ab95347</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

  • Carrier free

    Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8322(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/10000", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/10000", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hemoglobin subunit delta also known as Hb delta or delta hemoglobin is a component of the hemoglobin protein family which primarily includes hemoglobin subunit beta (Hb beta). Hemoglobin delta has a molecular mass of approximately 16 kDa. It expresses mainly in erythroid cells where it functions as part of the hemoglobin A2 (HbA2) molecule. HbA2 is a minor hemoglobin variant which includes two alpha globin chains and two delta globin chains. Delta hemoglobin plays a role in oxygen transport in red blood cells albeit to a lesser extent than the major form hemoglobin A (HbA) which contains hemoglobin subunit beta.
Biological function summary

Hemoglobin subunit delta participates in oxygen binding and transport. As part of the hemoglobin A2 complex it facilitates the pickup and release of oxygen molecules in tissues and organs. Although HbA2 only constitutes about 2-3% of total hemoglobin in adults it serves as an important backup system for effective oxygen delivery. The delta subunit closely interacts with partners like the alpha globin chains to perform its role in the hemoglobin complex.

Pathways

Hemoglobin subunit delta participates in the erythropoiesis pathway which is critical for red blood cell development. It works alongside proteins such as adult hemoglobin forms and alpha-like globins to ensure proper tissue oxygenation. The hypoxia-inducible factor pathway also involves hemoglobin components adjusting erythropoiesis in response to low oxygen levels though the direct role of delta hemoglobin in this adaptive process is comparatively minor.

Hemoglobin subunit delta has a connection with beta-thalassemia. This disorder results from mutations affecting beta globin synthesis leading to insufficient hemoglobin function. As a result HbA2 levels often rise as a compensatory mechanism. Sickle cell anemia mainly linked to mutations in the beta globin gene also exhibits some compensatory shifts in globin expression although delta hemoglobin itself is not directly involved. These disorders highlight the interconnected role of different hemoglobin chains including delta hemoglobin in red blood cell pathophysiology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in oxygen transport from the lung to the various peripheral tissues.
See full target information Hemoglobin subunit delta

Additional targets

HBB
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 11:5650-5674 PubMed33897873

2021

Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Zhang,Keke Yu,Lifang Ma,Zijun Qian,Xiaoting Tian,Yayou Miao,Yongjie Niu,Xin Xu,Susu Guo,Yueyue Yang,Zhixian Wang,Xiangfei Xue,Chuanjia Gu,Wentao Fang,Jiayuan Sun,Yongchun Yu,Jiayi Wang

Nature communications 11:6316 PubMed33298911

2020

TRIB3 promotes MYC-associated lymphoma development through suppression of UBE3B-mediated MYC degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Li,Feng Wang,Zhao-Na Yang,Ting-Ting Zhang,Yu-Fen Yuan,Chen-Xi Zhao,Zaiwuli Yeerjiang,Bing Cui,Fang Hua,Xiao-Xi Lv,Xiao-Wei Zhang,Jiao-Jiao Yu,Shan-Shan Liu,Jin-Mei Yu,Shuang Shang,Yang Xiao,Zhuo-Wei Hu
View all publications
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