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Rabbit Recombinant Monoclonal HBB antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

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Images

Western blot - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] - BSA and Azide free (AB248384), expandable thumbnail
  • Western blot - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] - BSA and Azide free (AB248384), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] - BSA and Azide free (AB248384), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] - BSA and Azide free (AB248384), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPIHC-PWB
Human
Not recommended
Tested
Tested

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Target data

Function

Involved in oxygen transport from the lung to the various peripheral tissues. LVV-hemorphin-7 potentiates the activity of bradykinin, causing a decrease in blood pressure. Spinorphin. Functions as an endogenous inhibitor of enkephalin-degrading enzymes such as DPP3, and as a selective antagonist of the P2RX3 receptor which is involved in pain signaling, these properties implicate it as a regulator of pain and inflammation.

Alternative names

Hemoglobin subunit beta, Beta-globin, Hemoglobin beta chain, HBB

Recommended products

Rabbit Recombinant Monoclonal HBB antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR8322(B)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab248384 is the carrier-free version of Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] ab131225.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Hemoglobin subunit delta also known as Hb delta or delta hemoglobin is a component of the hemoglobin protein family which primarily includes hemoglobin subunit beta (Hb beta). Hemoglobin delta has a molecular mass of approximately 16 kDa. It expresses mainly in erythroid cells where it functions as part of the hemoglobin A2 (HbA2) molecule. HbA2 is a minor hemoglobin variant which includes two alpha globin chains and two delta globin chains. Delta hemoglobin plays a role in oxygen transport in red blood cells albeit to a lesser extent than the major form hemoglobin A (HbA) which contains hemoglobin subunit beta.

Biological function summary

Hemoglobin subunit delta participates in oxygen binding and transport. As part of the hemoglobin A2 complex it facilitates the pickup and release of oxygen molecules in tissues and organs. Although HbA2 only constitutes about 2-3% of total hemoglobin in adults it serves as an important backup system for effective oxygen delivery. The delta subunit closely interacts with partners like the alpha globin chains to perform its role in the hemoglobin complex.

Pathways

Hemoglobin subunit delta participates in the erythropoiesis pathway which is critical for red blood cell development. It works alongside proteins such as adult hemoglobin forms and alpha-like globins to ensure proper tissue oxygenation. The hypoxia-inducible factor pathway also involves hemoglobin components adjusting erythropoiesis in response to low oxygen levels though the direct role of delta hemoglobin in this adaptive process is comparatively minor.

Associated diseases and disorders

Hemoglobin subunit delta has a connection with beta-thalassemia. This disorder results from mutations affecting beta globin synthesis leading to insufficient hemoglobin function. As a result HbA2 levels often rise as a compensatory mechanism. Sickle cell anemia mainly linked to mutations in the beta globin gene also exhibits some compensatory shifts in globin expression although delta hemoglobin itself is not directly involved. These disorders highlight the interconnected role of different hemoglobin chains including delta hemoglobin in red blood cell pathophysiology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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