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Rabbit Monoclonal Hemoglobin subunit zeta antibody. Suitable for IHC-P, WB and reacts with Human samples.

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Images

Western blot - Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (AB109444), expandable thumbnail
  • Western blot - Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (AB109444), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (AB109444), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (AB109444), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytICC/IFIHC-PWB
Human
Not recommended
Not recommended
Not recommended
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Predicted
Predicted
Rat
Not recommended
Not recommended
Not recommended
Predicted
Predicted

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/250.00000 - 1/500.00000

Notes

Antigen retrieval is recommended.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000.00000 - 1/10000.00000

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

1 product for Alternative Version

Target data

Function

The zeta chain is an alpha-type chain of mammalian embryonic hemoglobin.

Targets

Hemoglobin subunit alpha

Alternative names

HBZ2, HBZ2, HBZ, Hemoglobin subunit zeta, HBAZ, Hemoglobin zeta chain, Zeta-globin

Recommended products

Rabbit Monoclonal Hemoglobin subunit zeta antibody. Suitable for IHC-P, WB and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EP3607

Purification technique

Affinity purification Protein A

Specificity

Mouse and rat tissues tested (lung and kidney) gave negative or low sensitivity staining in IHC-P. This antibody may not be suitable for IHC with mouse or rat samples. If you have any questions, please contact our Scientific Support team.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Hemoglobin subunit zeta (HBZ) and hemoglobin subunit alpha (HBA) are components of the hemoglobin complex primarily known for their role in oxygen transport. HBZ often referred to as embryonic hemoglobin typically predominates in early human development. HBA on the other hand persists throughout life as part of the adult hemoglobin complex. HBA has a molecular mass of approximately 15.1 kDa. Both subunits are highly expressed in erythroid cells within the bone marrow.

Biological function summary

Hemoglobin subunits partake in forming the tetrameric hemoglobin complex essential for efficient oxygen loading and unloading at the tissues. HBZ and HBA interact with other globin chains like beta globin (HBB) to make different forms of hemoglobin. For embryos the functional presence of HBZ is important in facilitating oxygen transport in low-oxygen environments of the fetal placenta. HBA's expression continues postnatally and partners with HBB to achieve the classical hemoglobin A in red blood cells providing important oxygen delivery throughout life.

Pathways

These subunits are integral to the erythropoiesis pathway and the oxygen transport pathway. Hemoglobin biosynthesis is tightly regulated within erythropoiesis controlled by proteins such as erythropoietin and GATA1. The interaction and regulation of globin genes ensure the proper assembly of functional hemoglobin complexes. Additionally these pathways ensure balanced production of globin chains preventing disorders like thalassemia.

Associated diseases and disorders

Abnormalities in HBZ and HBA expression or mutations often link to conditions like thalassemia and sickle cell disease. Thalassemia arises from inadequate production of HBA or HBB causing imbalanced hemoglobin tetramer formation and anemia. In sickle cell disease abnormalities in the HBB chain alter hemoglobin structure affecting how HBA interacts with it. Both disorders highlight the importance of these protein interactions and their maintenance for proper erythrocyte functionality.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (ab109444), expandable thumbnail

    Western blot - Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (ab109444)

    All lanes: Western blot - Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (ab109444) at 1/1000 dilution

    Lane 1: His-tagged full length human Hemoglobin subunit alpha recombinant protein(Recombinant Human Hemoglobin subunit alpha protein (denatured) ab131697) at 0.01 µg

    Lane 2: His-tagged human Hemoglobin subunit beta/ba1 (aa3-147) recombinant protein at 0.01 µg

    Lane 3: His-tagged human Hemoglobin subunit delta (aa3-147) recombinant protein at 0.01 µg

    Lane 4: His-tagged human Hemoglobin subunit epsilon (aa3-147) recombinant protein at 0.01 µg

    Lane 5: His-tagged human Hemoglobin subunit Gamma-2 (aa2-147) recombinant protein at 0.01 µg

    Lane 6: His-tagged human Hemoglobin subunit Gamma-1 (aa2-147) recombinant protein at 0.01 µg

    Lane 7: His-tagged full length human Hemoglobin subunit Zeta recombinant protein(Recombinant Human Hemoglobin subunit zeta protein ab95347) at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 14 kDa, 20 kDa

  • Western blot - Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (ab109444), expandable thumbnail

    Western blot - Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (ab109444)

    All lanes: Western blot - Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (ab109444) at 1/1000 dilution

    Lane 1: K562 lysate at 10 µg

    Lane 2: Fetal liver lysate at 10 µg

    Lane 3: Fetal heart lysates at 10 µg

    Secondary

    All lanes: Standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Observed band size: 14 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (ab109444), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (ab109444)

    Immunohistochemical analysis of Hemoglobin subunit alpha and Hemoglobin subunit zeta in paraffin embedded human spleen tissue, using ab109444 at a 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (ab109444), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (ab109444)

    Immunohistochemical analysis of Hemoglobin subunit alpha and Hemoglobin subunit zeta in paraffin embedded human colon tissue, using ab109444 at a 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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