Rabbit Monoclonal Hemoglobin subunit zeta antibody. Suitable for IHC-P, WB and reacts with Human samples.
Images
![Western blot - Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (AB109444), expandable thumbnail](https://content.abcam.com/products/images/hemoglobin-subunit-zeta-hemoglobin-subunit-alpha-antibody-ep3607-ab109444--western-blot-img171992.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (AB109444)")
![Western blot - Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (AB109444), expandable thumbnail](https://content.abcam.com/products/images/hemoglobin-subunit-zeta-hemoglobin-subunit-alpha-antibody-ep3607-ab109444--western-blot-img106805.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (AB109444)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (AB109444), expandable thumbnail](https://content.abcam.com/products/images/hemoglobin-subunit-zeta-hemoglobin-subunit-alpha-antibody-ep3607-ab109444--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img103128.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (AB109444)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (AB109444), expandable thumbnail](https://content.abcam.com/products/images/hemoglobin-subunit-zeta-hemoglobin-subunit-alpha-antibody-ep3607-ab109444--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110712.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (AB109444)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IP | Flow Cyt | ICC/IF | IHC-P | WB | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Predicted | Predicted |
| Rat | Not recommended | Not recommended | Not recommended | Predicted | Predicted |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250.00000 - 1/500.00000 | Notes Antigen retrieval is recommended. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
The zeta chain is an alpha-type chain of mammalian embryonic hemoglobin.
Additional Targets
HBA1
Alternative names
HBZ2, HBZ, Hemoglobin subunit zeta, HBAZ, Hemoglobin zeta chain, Zeta-globin
Recommended products
Rabbit Monoclonal Hemoglobin subunit zeta antibody. Suitable for IHC-P, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP3607
- Purification technique
- Affinity purification Protein A
- Specificity
Mouse and rat tissues tested (lung and kidney) gave negative or low sensitivity staining in IHC-P. This antibody may not be suitable for IHC with mouse or rat samples. If you have any questions, please contact our Scientific Support team.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Hemoglobin subunit zeta (HBZ) and hemoglobin subunit alpha (HBA) are components of the hemoglobin complex primarily known for their role in oxygen transport. HBZ often referred to as embryonic hemoglobin typically predominates in early human development. HBA on the other hand persists throughout life as part of the adult hemoglobin complex. HBA has a molecular mass of approximately 15.1 kDa. Both subunits are highly expressed in erythroid cells within the bone marrow.
- Biological function summary
Hemoglobin subunits partake in forming the tetrameric hemoglobin complex essential for efficient oxygen loading and unloading at the tissues. HBZ and HBA interact with other globin chains like beta globin (HBB) to make different forms of hemoglobin. For embryos the functional presence of HBZ is important in facilitating oxygen transport in low-oxygen environments of the fetal placenta. HBA's expression continues postnatally and partners with HBB to achieve the classical hemoglobin A in red blood cells providing important oxygen delivery throughout life.
- Pathways
These subunits are integral to the erythropoiesis pathway and the oxygen transport pathway. Hemoglobin biosynthesis is tightly regulated within erythropoiesis controlled by proteins such as erythropoietin and GATA1. The interaction and regulation of globin genes ensure the proper assembly of functional hemoglobin complexes. Additionally these pathways ensure balanced production of globin chains preventing disorders like thalassemia.
- Associated diseases and disorders
Abnormalities in HBZ and HBA expression or mutations often link to conditions like thalassemia and sickle cell disease. Thalassemia arises from inadequate production of HBA or HBB causing imbalanced hemoglobin tetramer formation and anemia. In sickle cell disease abnormalities in the HBB chain alter hemoglobin structure affecting how HBA interacts with it. Both disorders highlight the importance of these protein interactions and their maintenance for proper erythrocyte functionality.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (ab109444), expandable thumbnail](https://content.abcam.com/products/images/hemoglobin-subunit-zeta-hemoglobin-subunit-alpha-antibody-ep3607-ab109444--western-blot-img171992.jpg "Western blot - Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (ab109444)")
Western blot - Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (ab109444)
All lanes: Western blot - Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (ab109444) at 1/1000 dilution
Lane 1: His-tagged full length human Hemoglobin subunit alpha recombinant protein(Recombinant Human Hemoglobin subunit alpha protein (denatured) ab131697) at 0.01 µg
Lane 2: His-tagged human Hemoglobin subunit beta/ba1 (aa3-147) recombinant protein at 0.01 µg
Lane 3: His-tagged human Hemoglobin subunit delta (aa3-147) recombinant protein at 0.01 µg
Lane 4: His-tagged human Hemoglobin subunit epsilon (aa3-147) recombinant protein at 0.01 µg
Lane 5: His-tagged human Hemoglobin subunit Gamma-2 (aa2-147) recombinant protein at 0.01 µg
Lane 6: His-tagged human Hemoglobin subunit Gamma-1 (aa2-147) recombinant protein at 0.01 µg
Lane 7: His-tagged full length human Hemoglobin subunit Zeta recombinant protein(Recombinant Human Hemoglobin subunit zeta protein ab95347) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 14 kDa, 20 kDa
![Western blot - Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (ab109444), expandable thumbnail](https://content.abcam.com/products/images/hemoglobin-subunit-zeta-hemoglobin-subunit-alpha-antibody-ep3607-ab109444--western-blot-img106805.jpg "Western blot - Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (ab109444)")
Western blot - Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (ab109444)
All lanes: Western blot - Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (ab109444) at 1/1000 dilution
Lane 1: K562 lysate at 10 µg
Lane 2: Fetal liver lysate at 10 µg
Lane 3: Fetal heart lysates at 10 µg
SecondaryAll lanes: Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Observed band size: 14 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (ab109444), expandable thumbnail](https://content.abcam.com/products/images/hemoglobin-subunit-zeta-hemoglobin-subunit-alpha-antibody-ep3607-ab109444--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img103128.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (ab109444)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (ab109444)
Immunohistochemical analysis of Hemoglobin subunit alpha and Hemoglobin subunit zeta in paraffin embedded human spleen tissue, using ab109444 at a 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (ab109444), expandable thumbnail](https://content.abcam.com/products/images/hemoglobin-subunit-zeta-hemoglobin-subunit-alpha-antibody-ep3607-ab109444--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110712.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (ab109444)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (ab109444)
Immunohistochemical analysis of Hemoglobin subunit alpha and Hemoglobin subunit zeta in paraffin embedded human colon tissue, using ab109444 at a 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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