AB239492

Anti-Hemojuvelin antibody [A7]

Name: Anti-Hemojuvelin antibody [A7] (ab239492) | Abcam
Brand: Abcam Limited
SKU: AB239492
Price: 525 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal Hemojuvelin antibody. Suitable for WB, IHC-P and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human HJV aa 1 to C-terminus.

View Alternative Names

HFE2, RGMC, HJV, Hemojuvelin, Hemochromatosis type 2 protein, Hemojuvelin BMP coreceptor, RGM domain family member C

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemojuvelin antibody [A7] (AB239492)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemojuvelin antibody [A7] (AB239492)

Paraffin-embedded human brain tissue stained for Hemojuvelin using ab239492 at 30 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemojuvelin antibody [A7] (AB239492)

Paraffin-embedded human liver cancer tissue stained for Hemojuvelin using ab239492 at 30 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemojuvelin antibody [A7] (AB239492)

Paraffin-embedded human prostate cancer tissue stained for Hemojuvelin using ab239492 at 30 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemojuvelin antibody [A7] (AB239492)

Paraffin-embedded human glioma tissue stained for Hemojuvelin using ab239492 at 30 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemojuvelin antibody [A7] (AB239492)

Paraffin-embedded human stomach cancer tissue stained for Hemojuvelin using ab239492 at 30 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemojuvelin antibody [A7] (AB239492)

Paraffin-embedded human breast cancer tissue stained for Hemojuvelin using ab239492 at 30 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-Hemojuvelin antibody [A7] (AB239492)

All lanes:

Western blot - Anti-Hemojuvelin antibody [A7] (ab239492) at 5 µg/mL

All lanes:

Recombinant human protein

Predicted band size: 45 kDa

false

Supplier Data

Western blot - Anti-Hemojuvelin antibody [A7] (AB239492)

All lanes:

Western blot - Anti-Hemojuvelin antibody [A7] (ab239492) at 2 µg/mL

All lanes:

Rat heart tissue lysate

Predicted band size: 45 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Isotype

IgG2b

Light chain type

kappa

Carrier free

Reacts with

Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human HJV aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q6ZVN8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-30 µg/mL", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from TCS.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hemojuvelin also known as HJV or RGMc is a glycosylphosphatidylinositol-anchored protein that plays a significant role in iron homeostasis. It has a molecular mass of about 42 kDa. Expression of hemojuvelin is mainly observed in the liver heart and skeletal muscles. This protein is essential for maintaining systemic iron levels and regulates how much iron is absorbed and stored in the body.

Biological function summary

Hemojuvelin interacts with other proteins to modulate iron metabolism. It acts as a coreceptor for bone morphogenetic proteins (BMPs) enhancing BMP signaling which is critical in regulating the peptide hormone hepcidin. Hemojuvelin itself is not part of a larger complex but it works closely with BMPs to control hepcidin expression. Hepcidin is the key hormone that dictates iron absorption and its release from macrophages maintaining iron balance.

Pathways

Hemojuvelin functions primarily within the BMP signaling and hepcidin regulatory pathways. The interaction with BMP6 is particularly critical. The BMP6-hemojuvelin complex boosts the SMAD signaling cascade resulting in increased hepcidin expression. This cascade impacts iron levels in the body by limiting intestinal iron absorption and controlling iron recycling by macrophages.

Hemojuvelin mutations can lead to juvenile hemochromatosis a condition characterized by excessive iron buildup in the body. This protein's dysfunction can severely affect iron homeostasis leading to detrimental health outcomes. Moreover its connection to the regulation of hepcidin links hemojuvelin to conditions like anemia of chronic disease where hepcidin expression is aberrantly increased or decreased. Understanding the interaction of hemojuvelin and hepcidin provides insights into the mechanisms behind these iron-related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a bone morphogenetic protein (BMP) coreceptor (PubMed : 18976966). Through enhancement of BMP signaling regulates hepcidin (HAMP) expression and regulates iron homeostasis (PubMed : 18976966).

See full target information HJV

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Digestive diseases and sciences : PubMed32440745

2020

Development of Pancreatic Acinar Cell Metaplasia During Gastric Repair in a Rat Duodenal Contents Reflux Model.

Applications

Unspecified application

Species

Unspecified reactive species

Yasuhiro Wada,Ken-Ichi Mukaisho,Shunpei Kanai,Takahisa Nakayama,Masahide Fukuda,Kazuhiro Mizukami,Tadayoshi Okimoto,Masaaki Kodama,Hiroyuki Sugihara,Kazunari Murakami,Ryoji Kushima

View all publications

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