Anti-Hemopexin antibody [EPR5610]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemopexin antibody [EPR5610] (AB124935)

ab124935, at 1/250 dilution, staining Hemopexin in paraffin-embedded Human liver tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Hemopexin antibody [EPR5610] (AB124935)

Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Hemopexin (red) with ab124935 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluorr^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Hemopexin antibody [EPR5610] (AB124935)

Immunocytochemistry analysis of HepG2 (human hepatocellular carcinoma epithelial cell) labeling Hemopexin with purified ab124935 at 1/200 dilution (10 µg/ml). Cells were fixed with 100% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.8 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control : PBS instead of the primary antibody.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemopexin antibody [EPR5610] (AB124935)

ab124935 staining Hemopexin in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 5% milk for 30 minutes at room temperature; antigen retrieval was by heat mediation in Tris pH 9.0. Samples were incubated with primary antibody (1/200 in PBS) for 16 hours at 4°C. An undiluted Biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Steffen Rickelt

• WB

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Western blot - Anti-Hemopexin antibody [EPR5610] (AB124935)

All lanes:

Western blot - Anti-Hemopexin antibody [EPR5610] (ab124935) at 1/10000 dilution

Lane 1:

Fetal liver lysate at 10 µg

Lane 2:

Human plasma lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

Predicted band size: 51 kDa

Observed band size: 70-75 kDa

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• OI-RD Scanning

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OI-RD Scanning - Anti-Hemopexin antibody [EPR5610] (AB124935)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.