JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB121991

Anti-HENMT1 antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal HENMT1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HENMT1 aa 1-100.

View Alternative Names

C1orf59, HENMT1, Small RNA 2'-O-methyltransferase, HEN1 methyltransferase homolog 1

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HENMT1 antibody (AB121991)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HENMT1 antibody (AB121991)

Immunohistochemical analysis of human testis tissue labeling HENMT1 with ab121991 at a 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HENMT1 antibody (AB121991)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HENMT1 antibody (AB121991)

Immunohistochemical analysis of human colon tissue labeling HENMT1 with ab121991 at a 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HENMT1 antibody (AB121991)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HENMT1 antibody (AB121991)

Immunohistochemical analysis of human pancreas tissue labeling HENMT1 with ab121991 at a 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HENMT1 antibody (AB121991)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HENMT1 antibody (AB121991)

Immunohistochemical analysis of human lymph node tissue labeling HENMT1 with ab121991 at a 1/200 dilution.

Western blot - Anti-HENMT1 antibody (AB121991)
  • WB

Unknown

Western blot - Anti-HENMT1 antibody (AB121991)

All lanes:

Western blot - Anti-HENMT1 antibody (ab121991) at 1/250 dilution

Lane 1:

Negative control (vector only transfected HEK293T lysate)

Lane 2:

Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells

Predicted band size: 45 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human HENMT1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q5T8I9

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HENMT1 also known as HEN1 methyltransferase homolog 1 is an enzyme with a molecular mass of approximately 55 kDa. It is expressed in the germ cells of both mammals and plants. This enzyme belongs to the small RNA methylation family and it plays an important role in the modification of small RNAs specifically piwi-interacting RNAs (piRNAs). HENMT1's primary function is transferring a methyl group to the 3' end of piRNAs protecting them against uridylation and exonucleolytic degradation keeping their integrity and stability necessary for their function.
Biological function summary

The enzyme HENMT1 participates in piRNA biogenesis. These piRNAs are part of a larger ribonucleoprotein complex that includes Piwi proteins which are essential for transposon silencing in the germline. This complex ensures the epigenetic and post-transcriptional silencing of transposable elements consequently protecting genomic integrity. HENMT1's activity is fundamental for safeguarding the genome from potential aberrations caused by active transposons.

Pathways

HENMT1 plays a significant part in the piRNA pathway which is important for gene silencing in the germline. Through this pathway it contributes to the protection of the genome against transposable elements by working alongside proteins like Piwi. The RNA interference (RNAi) pathway also involves HENMT1 although indirectly as RNAi and piRNA pathways share some mechanistic similarities in their mode of silencing unwanted genetic elements.

HENMT1 is connected to male infertility and certain cancers. Defects or malfunctions in HENMT1 can disrupt piRNA production leading to genomic instability and contributing to infertility in males. Additionally irregularities in its function might link it to cancer development possibly through faulty regulation of transposons. Other proteins like Piwi are relevant to understanding HENMT1's involvement in these conditions due to their collaborative function in the piRNA complex.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Methyltransferase that adds a 2'-O-methyl group at the 3'-end of piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. This probably protects the 3'-end of piRNAs from uridylation activity and subsequent degradation. Stabilization of piRNAs is essential for gametogenesis.
See full target information HENMT1
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Andrology 13:1137-1148 PubMed39120570

2024

Phenotypic continuum and poor intracytoplasmic sperm injection intracytoplasmic sperm injection prognosis in patients harboring HENMT1 variants.

Applications

Unspecified application

Species

Unspecified reactive species

Zeina Wehbe,Anne-Laure Barbotin,Angèle Boursier,Caroline Cazin,Jean-Pascal Hograindleur,Marie Bidart,Emeline Fontaine,Pauline Plouvier,Florence Puch,Véronique Satre,Christophe Arnoult,Selima Fourati Ben Mustapha,Raoudha Zouari,Nicolas Thierry-Mieg,Pierre F Ray,Zine-Eddine Kherraf,Charles Coutton,Guillaume Martinez

iScience 26:106663 PubMed37168556

2023

Whole-exome sequencing study of hypospadias.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongzhong Chen,Yunping Lei,Richard H Finnell,Yu Ding,Zhixi Su,Yaping Wang,Hua Xie,Fang Chen
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB166630

Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [EPR10823(B)] - Nucleolar Marker

RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [EPR10823(B)] - Nucleolar Marker (AB166630)

  • 5
  • 2
Primary Antibodies

AB154674

Anti-p38IP antibody

Western blot - Anti-p38IP antibody (AB154674)

  • 0
  • 0
Primary Antibodies

AB121348

Anti-FAM159B antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM159B antibody (AB121348)

  • 0
  • 1
Primary Antibodies

AB121532

Anti-FAM73A antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM73A antibody (AB121532)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com