JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB249724

Anti-HEPACAM antibody [EPR11217] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal HEPACAM antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Hepatic and glial cell adhesion molecule, glialCAM, Hepatocyte cell adhesion molecule, Protein hepaCAM, HEPACAM

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-HEPACAM antibody [EPR11217] - BSA and Azide free (AB249724)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HEPACAM antibody [EPR11217] - BSA and Azide free (AB249724)

This data was developed using ab172635, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of HepG2 cells labeling HEPACAM with ab172635 at 1/250 dilution. DAPI nuclear staining (blue).

Western blot - Anti-HEPACAM antibody [EPR11217] - BSA and Azide free (AB249724)
  • WB

Supplier Data

Western blot - Anti-HEPACAM antibody [EPR11217] - BSA and Azide free (AB249724)

This data was developed using ab172635, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HEPACAM antibody [EPR11217] (<a href='/en-us/products/primary-antibodies/hepacam-antibody-epr11217-ab172635'>ab172635</a>) at 1/1000 dilution

Lane 1:

Human fetal kidney lysate at 10 µg

Lane 2:

Human fetal liver lysate at 10 µg

Lane 3:

Human stomach lysate at 10 µg

Lane 4:

Human fetal brain lysate at 10 µg

Predicted band size: 46 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11217

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Primary Antibodies

AB154798

Anti-ClC-2 antibody [EPR6492(2)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ClC-2 antibody [EPR6492(2)] (AB154798)

  • 4
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB205198

Anti-AACT antibody [EPR17088-68]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-AACT antibody [EPR17088-68] (AB205198)

  • 0
  • 0
Primary Antibodies

AB181557

Anti-Oct4 antibody [EPR17929] - ChIP Grade

20ul selling size|RabMAb|Advanced Validation|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Oct4 antibody [EPR17929] - ChIP Grade (AB181557)

  • 5
  • 20
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
style
left-column

Product details

ab249724 is the carrier-free version of ab172635.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HEPACAM also known as "glialCAM" is a transmembrane glycoprotein that weighs approximately 55-65 kDa. It primarily appears in the glial cells of the central nervous system and plays a significant role in cell adhesion and communication. Researchers identified this protein in astrocytes oligodendrocytes and ependymal cells. It interacts with other cell adhesion molecules to mediate interactions between glial cells and contributes to maintaining the structural integrity of tissues.
Biological function summary

HEPACAM functions in the context of cell-cell adhesion and signal transduction mechanisms. This protein does not operate in isolation but rather forms part of larger protein complexes enhancing its role in cellular scaffolding and molecular signaling networks. The interaction with other cellular machinery supports processes like cell migration proliferation and differentiation which are critical for maintaining the homeostasis of neural tissues. Studies also highlight its role in modulating immune response and inflammatory processes within the nervous system.

Pathways

HEPACAM integrates into specific pathways involving cellular adhesion and signal transduction such as the PI3K/AKT signaling pathway. This pathway plays an important role in controlling cell growth and survival. HEPACAM's interaction with molecules like integrins and other cell adhesion proteins strengthens its function within this pathway. For instance integrins contribute to the regulation of the extracellular matrix composition affecting cellular movements and interactions vital for immune surveillance and responses in the nervous system.

Research associates HEPACAM with conditions like epilepsy and leukodystrophy. Alterations in HEPACAM expression or function can lead to improper cell adhesion disrupting normal tissue architecture and contributing to pathological states. Additionally there are links between HEPACAM and connexins proteins involved in forming gap junctions which further emphasizes its role in conditions characterized by impaired cellular communication. These associations highlight HEPACAM's involvement in maintaining neural stability and function within the central nervous system.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Involved in regulating cell motility and cell-matrix interactions. May inhibit cell growth through suppression of cell proliferation (PubMed : 15885354, PubMed : 15917256). In glia, associates and targets CLCN2 at astrocytic processes and myelinated fiber tracts where it may regulate transcellular chloride flux involved in neuron excitability (PubMed : 22405205).
See full target information HEPACAM
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oxidative medicine and cellular longevity 2021:8379962 PubMed34630853

2021

circ_0023461 Silencing Protects Cardiomyocytes from Hypoxia-Induced Dysfunction through Targeting miR-370-3p/PDE4D Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Ren,Buying Li,Liqing Jiang,Zhiheng Liu,Fan Wu,Yi Zhang,Jincheng Liu,Weixun Duan
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com