AB23418

Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A74]

Name: Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A74] (ab23418) | Abcam
Brand: Abcam Limited
SKU: AB23418
Price: 470 USD
Availability: InStock
Rating: 4 (6 reviews)

(6 Reviews)

(20 Publications)

Mouse Monoclonal Heparan Sulfate Proteoglycan 2/Perlecan antibody. Suitable for IP, ELISA, WB, IHC-P, ICC/IF, AP, IHC-Fr and reacts with Human, Cow samples. Cited in 20 publications. Immunogen corresponding to Cell preparation containing Heparan Sulfate Proteoglycan 2/Perlecan protein.

View Alternative Names

Basement membrane-specific heparan sulfate proteoglycan core protein, HSPG, Perlecan, PLC, HSPG2

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

A74

Isotype

IgG1

Carrier free

Reacts with

Cow, Human

Applications

WB, IP, IHC-P, ICC/IF, ELISA, AP, IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Epitope

Epitope is located in domain V of Perlecan.

Specificity

ab23418 is highly specific for perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin).

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 2.9% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Heparan Sulfate Proteoglycan 2 also known as Perlecan is a large multidomain proteoglycan with a mass of over 500 kDa. It contains heparan sulfate chains that contribute to its role in the extracellular matrix. Perlecan is expressed in various tissues including cartilage basement membranes and vascular tissues. It interacts with other matrix proteins providing structural integrity and influencing cell behavior.

Biological function summary

Perlecan plays a critical role in cell adhesion proliferation and growth factor signaling. It is an important component of basement membranes and forms a structural complex with other matrix proteins. Its interaction with heparan sulfate chains enables binding with various ligands such as growth factors and cytokines optimizing their availability and functionality at the cell surface. This interaction is essential in tissue maintenance and repair processes.

Pathways

Perlecan participates in the regulation of the Wnt and Hedgehog signaling pathways. These pathways influence key developmental processes and dictate cellular differentiation and migration. Perlecan and its heparan sulfate chains modulate protein interactions within these pathways notably affecting proteins such as Dsh and Ctnnb1 which are essential in signal transduction.

Perlecan involvement has been linked with tumorigenesis and cardiovascular diseases. Aberrations in its structure or expression can alter cellular microenvironments impacting key processes like angiogenesis and metastasis. Furthermore its interaction with growth factors can facilitate the progression of diseases like cancer highlighting its connection with proteins such as VEGF and TGF-beta which play major roles in disease modulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development.. Endorepellin. Anti-angiogenic and anti-tumor peptide that inhibits endothelial cell migration, collagen-induced endothelial tube morphogenesis and blood vessel growth in the chorioallantoic membrane. Blocks endothelial cell adhesion to fibronectin and type I collagen. Anti-tumor agent in neovascularization. Interaction with its ligand, integrin alpha2/beta1, is required for the anti-angiogenic properties. Evokes a reduction in phosphorylation of receptor tyrosine kinases via alpha2/beta1 integrin-mediated activation of the tyrosine phosphatase, PTPN6.. LG3 peptide. Has anti-angiogenic properties that require binding of calcium ions for full activity.

See full target information Basement membrane-specific heparan sulfate proteoglycan core protein

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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

Alzheimer's & dementia : the journal of the Alzheimer's Association 21:e70103 PubMed40189804

2025

Structural changes in cerebral microvasculature induced by ferroptosis contribute to blood-brain barrier disruption in Alzheimer's disease: an autopsy study.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Cao,Mei-Ying Huang,Chen-Hui Mao,Xue Wang,Yuan-Yuan Xu,Xiao-Jing Qian,Chao Ma,Wen-Ying Qiu,Yi-Cheng Zhu

International journal of nanomedicine 18:7047-7063 PubMed38046235

2023

Exosome Derived from Mesenchymal Stem Cells Alleviates Hypertrophic Scar by Inhibiting the Fibroblasts via TNFSF-13/HSPG2 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Huimin Zhang,Chengyu Zang,Wen Zhao,Linfeng Zhang,Rui Liu,Zhang Feng,Jie Wu,Rongtao Cui

Frontiers in medicine 10:891513 PubMed36860338

2023

The loss of glycocalyx integrity impairs complement factor H binding and contributes to cyclosporine-induced endothelial cell injury.

Applications

Unspecified application

Species

Unspecified reactive species

Chia Wei Teoh,Magdalena Riedl Khursigara,Carolina G Ortiz-Sandoval,Jee Woo Park,Jun Li,Arlette Bohorquez-Hernandez,Valentina Bruno,Emily E Bowen,Spencer A Freeman,Lisa A Robinson,Christoph Licht

Tissue barriers 11:2039003 PubMed35262466

2022

GRP75 as a functional element of cholix transcytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Keyi Liu,Tom Hunter,Alistair Taverner,Kevin Yin,Julia MacKay,Kate Colebrook,Morgan Correia,Amandine Rapp,Randall J Mrsny

Frontiers in cardiovascular medicine 8:736303 PubMed34527715

2021

Angiogenic Secretion Profile of Valvular Interstitial Cells Varies With Cellular Sex and Phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Victoria Nelson,Vaidehi Patil,LaTonya R Simon,Kelsey Schmidt,Chloe M McCoy,Kristyn S Masters

The Journal of biological chemistry 296:100520 PubMed33684447

2021

Macrophages bind LDL using heparan sulfate and the perlecan protein core.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Yi Ng,John M Whitelock,Helen Williams,Ha Na Kim,Heather J Medbury,Megan S Lord

Advanced healthcare materials 9:e1901669 PubMed32129003

2020

Extracellular Matrix Production by Mesenchymal Stromal Cells in Hydrogels Facilitates Cell Spreading and Is Inhibited by FGF-2.

Applications

Unspecified application

Species

Unspecified reactive species

Edward R Horton,Queralt Vallmajo-Martin,Ivan Martin,Jess G Snedeker,Martin Ehrbar,Ulrich Blache

Nature communications 10:3656 PubMed31409793

2019

A glomerulus-on-a-chip to recapitulate the human glomerular filtration barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Astgik Petrosyan,Paolo Cravedi,Valentina Villani,Andrea Angeletti,Joaquin Manrique,Alessandra Renieri,Roger E De Filippo,Laura Perin,Stefano Da Sacco

Journal of tissue engineering and regenerative medicine 13:1362-1374 PubMed31067012

2019

Influence of fibrin matrices and their released factors on epidermal substitute phenotype and engraftment.

Applications

Unspecified application

Species

Unspecified reactive species

Maia M Alexaline,Brice Magne,Amparo Zuleta Rodríguez,Muriel Nivet,Daniel Bacqueville,Jean-Jacques Lataillade,Marina Trouillas

Frontiers in bioengineering and biotechnology 6:156 PubMed30410879

2018

Microvascular Networks From Endothelial Cells and Mesenchymal Stromal Cells From Adipose Tissue and Bone Marrow: A Comparison.

Applications

Unspecified application

Species

Unspecified reactive species

Karoline Pill,Johanna Melke,Severin Mühleder,Marianne Pultar,Sabrina Rohringer,Eleni Priglinger,Heinz R Redl,Sandra Hofmann,Wolfgang Holnthoner

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A74]

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (20)

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