AB26265

Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A76]

Name: Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A76] (ab26265) | Abcam
Brand: Abcam Limited
SKU: AB26265
Price: 450 USD
Availability: InStock

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(8 Publications)

Mouse Monoclonal Heparan Sulfate Proteoglycan 2/Perlecan antibody. Suitable for IP, ELISA, WB, IHC-P, AP, IHC-Fr and reacts with Human, Cow, Sheep samples. Cited in 8 publications. Immunogen corresponding to Cell preparation containing Heparan Sulfate Proteoglycan 2/Perlecan protein.

View Alternative Names

Basement membrane-specific heparan sulfate proteoglycan core protein, HSPG, Perlecan, PLC, HSPG2

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

A76

Isotype

IgG1

Carrier free

Reacts with

Cow, Human, Sheep

Applications

AP, ELISA, IP, IHC-P, IHC-Fr, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is highly specific for Heparan Sulfate Proteoglycan 2/Perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin) but it does cross-react with human thrombospondin.

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Protein-A/G purified.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 2.9% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Heparan Sulfate Proteoglycan 2 also known as Perlecan is a large multidomain proteoglycan with a mass of over 500 kDa. It contains heparan sulfate chains that contribute to its role in the extracellular matrix. Perlecan is expressed in various tissues including cartilage basement membranes and vascular tissues. It interacts with other matrix proteins providing structural integrity and influencing cell behavior.

Biological function summary

Perlecan plays a critical role in cell adhesion proliferation and growth factor signaling. It is an important component of basement membranes and forms a structural complex with other matrix proteins. Its interaction with heparan sulfate chains enables binding with various ligands such as growth factors and cytokines optimizing their availability and functionality at the cell surface. This interaction is essential in tissue maintenance and repair processes.

Pathways

Perlecan participates in the regulation of the Wnt and Hedgehog signaling pathways. These pathways influence key developmental processes and dictate cellular differentiation and migration. Perlecan and its heparan sulfate chains modulate protein interactions within these pathways notably affecting proteins such as Dsh and Ctnnb1 which are essential in signal transduction.

Perlecan involvement has been linked with tumorigenesis and cardiovascular diseases. Aberrations in its structure or expression can alter cellular microenvironments impacting key processes like angiogenesis and metastasis. Furthermore its interaction with growth factors can facilitate the progression of diseases like cancer highlighting its connection with proteins such as VEGF and TGF-beta which play major roles in disease modulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development.. Endorepellin. Anti-angiogenic and anti-tumor peptide that inhibits endothelial cell migration, collagen-induced endothelial tube morphogenesis and blood vessel growth in the chorioallantoic membrane. Blocks endothelial cell adhesion to fibronectin and type I collagen. Anti-tumor agent in neovascularization. Interaction with its ligand, integrin alpha2/beta1, is required for the anti-angiogenic properties. Evokes a reduction in phosphorylation of receptor tyrosine kinases via alpha2/beta1 integrin-mediated activation of the tyrosine phosphatase, PTPN6.. LG3 peptide. Has anti-angiogenic properties that require binding of calcium ions for full activity.

See full target information Basement membrane-specific heparan sulfate proteoglycan core protein

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Biofabrication 13: PubMed33910175

2021

A perfusable vascularized full-thickness skin model for potential topical and systemic applications.

Applications

Unspecified application

Species

Unspecified reactive species

Sacha Salameh,Nicolas Tissot,Kevin Cache,Joaquim Lima,Itaru Suzuki,Paulo André Marinho,Maité Rielland,Jérémie Soeur,Shoji Takeuchi,Stéphane Germain,Lionel Breton

Life (Basel, Switzerland) 11: PubMed33668945

2021

Lectin Staining of Microvascular Glycocalyx in Microfluidic Cancer Cell Extravasation Assays.

Applications

Unspecified application

Species

Unspecified reactive species

Sebastian Beyer,Anna Blocki,Matthew Chung Yin Cheung,Zoe Ho Ying Wan,Babak Mehrjou,Roger Dale Kamm

Journal of molecular histology 50:285-294 PubMed30993430

2019

Type XI collagen-perlecan-HS interactions stabilise the pericellular matrix of annulus fibrosus cells and chondrocytes providing matrix stabilisation and homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Susan M Smith,James Melrose

Development (Cambridge, England) 145: PubMed29180569

2018

The skeletal phenotype of achondrogenesis type 1A is caused exclusively by cartilage defects.

Applications

Unspecified application

Species

Unspecified reactive species

Ian M Bird,Susie H Kim,Devin K Schweppe,Joana Caetano-Lopes,Alexander G Robling,Julia F Charles,Steven P Gygi,Matthew L Warman,Patrick J Smits

Biomaterials 67:354-64 PubMed26241755

2015

3-Dimensional spatially organized PEG-based hydrogels for an aortic valve co-culture model.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel S Puperi,Liezl R Balaoing,Ronan W O'Connell,Jennifer L West,K Jane Grande-Allen

Histochemistry and cell biology 134:251-63 PubMed20690028

2010

Comparative immunolocalisation of perlecan with collagen II and aggrecan in human foetal, newborn and adult ovine joint tissues demonstrates perlecan as an early developmental chondrogenic marker.

Applications

IHC-P, IHC-P

Species

Sheep, Human

Susan M Smith,Cindy Shu,James Melrose

Matrix biology : journal of the International Society for Matrix Biology 29:63-73 PubMed19788922

2009

Myeloperoxidase-derived oxidants selectively disrupt the protein core of the heparan sulfate proteoglycan perlecan.

Applications

WB, ELISA

Species

Human, Human

Martin D Rees,John M Whitelock,Ernst Malle,Christine Y Chuang,Renato V Iozzo,Anastasia Nilasaroya,Michael J Davies

Biotechnic & histochemistry : official publication of the Biological Stain Commission 83:47-53 PubMed18568676

2008

The use of Histochoice for histological examination of articular and growth plate cartilages, intervertebral disc and meniscus.

Applications

IHC-P

Species

Sheep

J Melrose,S M Smith,M M Smith,C B Little

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A76]

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

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