Rat Monoclonal Heparan Sulfate Proteoglycan 2/Perlecan antibody. Suitable for ICC, IP, WB, IHC-P, IHC-Fr and reacts with Mouse, Rat, Pig, Fish, Human, Cow samples. Cited in 33 publications. Immunogen corresponding to Tissue preparation containing Basement membrane-specific heparan sulfate proteoglycan core protein protein.
Images
![Immunohistochemistry (Frozen sections) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A7L6] (AB2501), expandable thumbnail](https://content.abcam.com/products/images/heparan-sulfate-proteoglycan-2-perlecan-antibody-a7l6-ab2501--immunohistochemistry-frozen-sections-img91054.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A7L6] (AB2501)")
![Immunohistochemistry (Frozen sections) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A7L6] (AB2501), expandable thumbnail](https://content.abcam.com/products/images/heparan-sulfate-proteoglycan-2-perlecan-antibody-a7l6-ab2501--immunohistochemistry-frozen-sections-img87815.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A7L6] (AB2501)")
Publications
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- International journal of molecular sciences 24:2023Regulation of Glycoprotein VI-Dependent Platelet Activation and Thrombus Formation by Heparan Sulfate Proteoglycan Perlecan.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIsabella Provenzale,Ilaria De Simone,Jonathan M Gibbins,Johan W M Heemskerk,Paola E J van der Meijden,Chris I JonesPubMed 37686158
- Nature immunology 24:414-4222023IFNγ binding to extracellular matrix prevents fatal systemic toxicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJosephine Kemna,Evelyne Gout,Leon Daniau,Jessica Lao,Kristoffer Weißert,Sandra Ammann,Ralf Kühn,Matthias Richter,Christine Molenda,Anje Sporbert,Dario Zocholl,Robert Klopfleisch,Anja Schütz,Hugues Lortat-Jacob,Peter Aichele,Thomas Kammertoens,Thomas BlankensteinPubMed 36732425
- Journal of inflammation research 16:421-4312023Protectin DX Relieve Hyperoxia-induced Lung Injury by Protecting Pulmonary Endothelial Glycocalyx.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhongjie Liang,Huilin Yue,Congcong Xu,Qian Wang,Shengwei JinPubMed 36755970
- ACS biomaterials science & engineering 8:2574-25882022Peripheral Nerve Decellularization for Extracellular Matrix Hydrogel Use: A Comparative Study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmory Gregory,In Ha Baek,Nikolas Ala-Kokko,Reagan Dugan,Luis Pinzon-Herrera,Jorge Almodóvar,Young Hye SongPubMed 35649243
- JBMR plus 6:e105682022Longitudinal Imaging of the Skull Base Synchondroses Demonstrate Prevention of a Premature Ossification After Recifercept Treatment in Mouse Model of Achondroplasia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuylene Rignol,Stephanie Garcia,Florence Authier,Kaamula Smith,Lionel Tosello,Raphael Marsault,Pierre Dellugat,Diogo Goncalves,Marlene Brouillard,Jeffrey Stavenhagen,Luca Santarelli,Christian Czech,Elvire GouzePubMed 35229060
- International journal of molecular sciences 22:2021Intramuscular Exposure to a Lethal Dose of Ricin Toxin Leads to Endothelial Glycocalyx Shedding and Microvascular Flow Abnormality in Mice and Swine.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnita Sapoznikov,Yoav Gal,Yentl Evgy,Moshe Aftalion,Shahaf Katalan,Tamar Sabo,Chanoch Kronman,Reut FalachPubMed 34830227
- Bioelectricity 3:204-2142021Lung Ablation with Irreversible Electroporation Promotes Immune Cell Infiltration by Sparing Extracellular Matrix Proteins and Vasculature: Implications for Immunotherapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMasashi Fujimori,Yasushi Kimura,Eisuke Ueshima,Damian E Dupuy,Prasad S Adusumilli,Stephen B Solomon,Govindarajan SrimathveeravalliPubMed 34734168
- European cells & materials 41:73-8920213D distribution of perlecan within intervertebral disc chondrons suggests novel regulatory roles for this multifunctional modular heparan sulphate proteoglycan.Applications:Unspecified applicationReactive species:Unspecified reactive speciesA J Hayes,J MelrosePubMed 33454951
- Fluids and barriers of the CNS 17:602020The microvascular extracellular matrix in brains with Alzheimer's disease neuropathologic change (ADNC) and cerebral amyloid angiopathy (CAA).Applications:Unspecified applicationReactive species:Unspecified reactive speciesMamatha Damodarasamy et. al.PubMed 32993718
- Chinese medical journal 134:212-2182020Mitochondrial transfer from bone-marrow-derived mesenchymal stromal cells to chondrocytes protects against cartilage degenerative mitochondrial dysfunction in rats chondrocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRui Wang et. al.PubMed 32858593
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Tissue preparation containing Basement membrane-specific heparan sulfate proteoglycan core protein protein. The exact immunogen used to generate this antibody is proprietary information. Database link P98160
Reactivity data
Select an application| ICC | IP | WB | IHC-P | IHC-Fr | |
|---|---|---|---|---|---|
| Human | Expected | Expected | Expected | Expected | Tested |
| Mouse | Expected | Expected | Expected | Expected | Predicted |
| Rat | Expected | Expected | Expected | Expected | Predicted |
| Cow | Expected | Expected | Expected | Expected | Predicted |
| Fish | Expected | Expected | Expected | Expected | Predicted |
| Pig | Expected | Expected | Expected | Expected | Predicted |
ICC
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Use amplification with ABC (avidin biotin complex). |
Species Rat | Dilution info - | Notes Use amplification with ABC (avidin biotin complex). |
Species Pig | Dilution info - | Notes Use amplification with ABC (avidin biotin complex). |
Species Fish | Dilution info - | Notes Use amplification with ABC (avidin biotin complex). |
Species Human | Dilution info - | Notes Use amplification with ABC (avidin biotin complex). |
Species Cow | Dilution info - | Notes Use amplification with ABC (avidin biotin complex). |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Pig, Fish, Human, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Pig, Fish, Human, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Use amplification with ABC (avidin biotin complex). |
Species Rat | Dilution info - | Notes Use amplification with ABC (avidin biotin complex). |
Species Pig | Dilution info - | Notes Use amplification with ABC (avidin biotin complex). |
Species Fish | Dilution info - | Notes Use amplification with ABC (avidin biotin complex). |
Species Human | Dilution info - | Notes Use amplification with ABC (avidin biotin complex). |
Species Cow | Dilution info - | Notes Use amplification with ABC (avidin biotin complex). |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Cow, Fish, Pig | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development. Endorepellin. Anti-angiogenic and anti-tumor peptide that inhibits endothelial cell migration, collagen-induced endothelial tube morphogenesis and blood vessel growth in the chorioallantoic membrane. Blocks endothelial cell adhesion to fibronectin and type I collagen. Anti-tumor agent in neovascularization. Interaction with its ligand, integrin alpha2/beta1, is required for the anti-angiogenic properties. Evokes a reduction in phosphorylation of receptor tyrosine kinases via alpha2/beta1 integrin-mediated activation of the tyrosine phosphatase, PTPN6. LG3 peptide. Has anti-angiogenic properties that require binding of calcium ions for full activity.
Alternative names
Basement membrane-specific heparan sulfate proteoglycan core protein, HSPG, Perlecan, PLC, HSPG2
Recommended products
Rat Monoclonal Heparan Sulfate Proteoglycan 2/Perlecan antibody. Suitable for ICC, IP, WB, IHC-P, IHC-Fr and reacts with Mouse, Rat, Pig, Fish, Human, Cow samples. Cited in 33 publications. Immunogen corresponding to Tissue preparation containing Basement membrane-specific heparan sulfate proteoglycan core protein protein.
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Tissue preparation containing Basement membrane-specific heparan sulfate proteoglycan core protein protein. The exact immunogen used to generate this antibody is proprietary information. Database link P98160
- Clone number
- A7L6
- Purification technique
- Affinity purification Protein A
- Specificity
Recognizes domain IV of heparan sulphate proteoglycan 2 / perlecan. The reactivity is independent of the galactosaminoglycan moieties. Therefore, the epitope is not sensitive to heparitinase treatment.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Proteoglycans are macromolecules consisting of a variety of core proteins with covalently attached one or several polysaccharide chains of the glycosaminoglycan type (heparan sulphate, heparin, chondroitin sulphate, dermatan sulphate or keratan sulphate). At least two forms of basement membrane heparan sulphate proteoglycan (HSPG) have been identified. One with a large core protein (> 400 kD) and one with a small core protein (30 kD). The large HSPG is probably the most abundant basement membrane proteoglycan. It is located predominantly in the lamina lucida, where it forms clustered aggregates and interacts with other basement membrane components to form the matrix. In addition, it also plays a critical role in attachment of cells to the basal membrane via integrin receptors.
Source: A7L6 is a Rat monoclonal IgG2a antibody derived by fusion of X63 Ag8.653 Mouse myeloma cells with spleen cells from a Fisher Rat immunized with high molecular mass material derived from the Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and HSPG.
Formulation: The vial contains 100 ul 1 mg/ml monoclonal purified antibody in PBS containing 0.09% sodium azide.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Heparan Sulfate Proteoglycan 2 also known as Perlecan is a large multidomain proteoglycan with a mass of over 500 kDa. It contains heparan sulfate chains that contribute to its role in the extracellular matrix. Perlecan is expressed in various tissues including cartilage basement membranes and vascular tissues. It interacts with other matrix proteins providing structural integrity and influencing cell behavior.
- Biological function summary
Perlecan plays a critical role in cell adhesion proliferation and growth factor signaling. It is an important component of basement membranes and forms a structural complex with other matrix proteins. Its interaction with heparan sulfate chains enables binding with various ligands such as growth factors and cytokines optimizing their availability and functionality at the cell surface. This interaction is essential in tissue maintenance and repair processes.
- Pathways
Perlecan participates in the regulation of the Wnt and Hedgehog signaling pathways. These pathways influence key developmental processes and dictate cellular differentiation and migration. Perlecan and its heparan sulfate chains modulate protein interactions within these pathways notably affecting proteins such as Dsh and Ctnnb1 which are essential in signal transduction.
- Associated diseases and disorders
Perlecan involvement has been linked with tumorigenesis and cardiovascular diseases. Aberrations in its structure or expression can alter cellular microenvironments impacting key processes like angiogenesis and metastasis. Furthermore its interaction with growth factors can facilitate the progression of diseases like cancer highlighting its connection with proteins such as VEGF and TGF-beta which play major roles in disease modulation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
![Immunohistochemistry (Frozen sections) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A7L6] (ab2501), expandable thumbnail](https://content.abcam.com/products/images/heparan-sulfate-proteoglycan-2-perlecan-antibody-a7l6-ab2501--immunohistochemistry-frozen-sections-img91054.jpg "Immunohistochemistry (Frozen sections) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A7L6] (ab2501)")
Immunohistochemistry (Frozen sections) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A7L6] (ab2501)
IHC image on a frozen section of human colon showing strong reactivity in the extracellular matrix and basement membrane.
![Immunohistochemistry (Frozen sections) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A7L6] (ab2501), expandable thumbnail](https://content.abcam.com/products/images/heparan-sulfate-proteoglycan-2-perlecan-antibody-a7l6-ab2501--immunohistochemistry-frozen-sections-img87815.jpg "Immunohistochemistry (Frozen sections) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A7L6] (ab2501)")
Immunohistochemistry (Frozen sections) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A7L6] (ab2501)
Immunohistochemistry on frozen section of Human kidney showing strong reactivity in the extracellular matrix and basement membrane.
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