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AB2501

Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A7L6]

5

(10 Reviews)

|

(39 Publications)

Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A7L6] (ab2501) is a rat monoclonal antibody detecting Heparan Sulfate Proteoglycan 2/Perlecan in Western Blot, IP, IHC-P, IHC-Fr, ICC. Suitable for Cow, Fish, Human, Mouse, Pig, Rat.

- Over 30 publications
- Trusted since 2002

View Alternative Names

Basement membrane-specific heparan sulfate proteoglycan core protein, HSPG, Perlecan, PLC, HSPG2

2 Images
Immunohistochemistry (Frozen sections) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A7L6] (AB2501)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A7L6] (AB2501)

IHC image on a frozen section of human colon showing strong reactivity in the extracellular matrix and basement membrane.

Immunohistochemistry (Frozen sections) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A7L6] (AB2501)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A7L6] (AB2501)

Immunohistochemistry on frozen section of Human kidney showing strong reactivity in the extracellular matrix and basement membrane.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

A7L6

Isotype

IgG2a

Carrier free

No

Reacts with

Human, Rat, Mouse, Cow, Fish, Pig

Applications

WB, IHC-P, IP, ICC, IHC-Fr

applications

Immunogen

Tissue preparation containing Basement membrane-specific heparan sulfate proteoglycan core protein protein. The exact immunogen used to generate this antibody is proprietary information.

P98160

Specificity

Recognizes domain IV of heparan sulphate proteoglycan 2 / perlecan. The reactivity is independent of the galactosaminoglycan moieties. Therefore, the epitope is not sensitive to heparitinase treatment.

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Primary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p>Use amplification with ABC (avidin biotin complex).</p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Use amplification with ABC (avidin biotin complex).</p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p>Use amplification with ABC (avidin biotin complex).</p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Use amplification with ABC (avidin biotin complex).</p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Rat": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p>Use amplification with ABC (avidin biotin complex).</p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Use amplification with ABC (avidin biotin complex).</p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Cow": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p>Use amplification with ABC (avidin biotin complex).</p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Use amplification with ABC (avidin biotin complex).</p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Fish": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p>Use amplification with ABC (avidin biotin complex).</p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Use amplification with ABC (avidin biotin complex).</p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Pig": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p>Use amplification with ABC (avidin biotin complex).</p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Use amplification with ABC (avidin biotin complex).</p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A7L6] (ab2501) is a rat monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Cow, Fish, Human, Mouse, Pig, Rat samples.

Trusted by the scientific community
Anti-Heparan Sulfate Proteoglycan 2/Perlecan [A7L6] (ab2501) was first used in a scientific publication in 2002 and has been cited over 30 times in peer-reviewed journals.

Reviewed by scientists
Anti-Heparan Sulfate Proteoglycan 2/Perlecan [A7L6] (ab2501) has over 10 independent reviews from customers.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Heparan Sulfate Proteoglycan 2 also known as Perlecan is a large multidomain proteoglycan with a mass of over 500 kDa. It contains heparan sulfate chains that contribute to its role in the extracellular matrix. Perlecan is expressed in various tissues including cartilage basement membranes and vascular tissues. It interacts with other matrix proteins providing structural integrity and influencing cell behavior.
Biological function summary

Perlecan plays a critical role in cell adhesion proliferation and growth factor signaling. It is an important component of basement membranes and forms a structural complex with other matrix proteins. Its interaction with heparan sulfate chains enables binding with various ligands such as growth factors and cytokines optimizing their availability and functionality at the cell surface. This interaction is essential in tissue maintenance and repair processes.

Pathways

Perlecan participates in the regulation of the Wnt and Hedgehog signaling pathways. These pathways influence key developmental processes and dictate cellular differentiation and migration. Perlecan and its heparan sulfate chains modulate protein interactions within these pathways notably affecting proteins such as Dsh and Ctnnb1 which are essential in signal transduction.

Perlecan involvement has been linked with tumorigenesis and cardiovascular diseases. Aberrations in its structure or expression can alter cellular microenvironments impacting key processes like angiogenesis and metastasis. Furthermore its interaction with growth factors can facilitate the progression of diseases like cancer highlighting its connection with proteins such as VEGF and TGF-beta which play major roles in disease modulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development (PubMed : 12435733, PubMed : 15591058, PubMed : 19789387). In muscle, it is essential for localizing acetylcholinesterase (AChE) at the neuromuscular junctions (NMJ), most probably acting as an adapter that links the acetylcholinesterase collagenic tail peptide (COLQ) to alpha-dystroglycan, and is therefore involved in the down-regulation of colinergic synaptic transmission (By similarity).. Endorepellin. Anti-angiogenic and anti-tumor peptide that inhibits endothelial cell migration, collagen-induced endothelial tube morphogenesis and blood vessel growth in the chorioallantoic membrane. Blocks endothelial cell adhesion to fibronectin and type I collagen. Anti-tumor agent in neovascularization. Interaction with its ligand, integrin alpha2/beta1, is required for the anti-angiogenic properties. Evokes a reduction in phosphorylation of receptor tyrosine kinases via alpha2/beta1 integrin-mediated activation of the tyrosine phosphatase, PTPN6.. LG3 peptide. Has anti-angiogenic properties that require binding of calcium ions for full activity.
See full target information Basement membrane-specific heparan sulfate proteoglycan core protein
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Publications (39)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 24:345 PubMed39438949

2024

Identification of HSPG2 as a bladder pro-tumor protein through NID1/AKT signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Cong Li,Pengwei Luo,Fengzhu Guo,Xu Jia,Min Shen,Ting Zhang,Shusen Wang,Ting Du

Circulation. Heart failure 17:e011504 PubMed38910562

2024

Overexpression of in Cardiomyocytes Promotes Cardiac Reverse Remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Mengda Xu,Hang Zhang,Yuan Chang,Xiumeng Hua,Xiao Chen,Yixuan Sheng,Dan Shan,Mengni Bao,Shengshou Hu,Jiangping Song

Cell communication and signaling : CCS 22:191 PubMed38528533

2024

Endothelial CXCR2 deficiency attenuates renal inflammation and glycocalyx shedding through NF-κB signaling in diabetic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Siyuan Cui,Xin Chen,Jiayu Li,Wei Wang,Deqi Meng,Shenglong Zhu,Shiwei Shen

International journal of molecular sciences 24: PubMed37686158

2023

Regulation of Glycoprotein VI-Dependent Platelet Activation and Thrombus Formation by Heparan Sulfate Proteoglycan Perlecan.

Applications

Unspecified application

Species

Unspecified reactive species

Isabella Provenzale,Ilaria De Simone,Jonathan M Gibbins,Johan W M Heemskerk,Paola E J van der Meijden,Chris I Jones

Matrix biology : journal of the International Society for Matrix Biology 118:92-109 PubMed36907428

2023

Conditional expression of endorepellin in the tumor vasculature attenuates breast cancer growth, angiogenesis and hyaluronan deposition.

Applications

Unspecified application

Species

Unspecified reactive species

Carolyn G Chen,Aastha Kapoor,Christopher Xie,Alison Moss,Rajanikanth Vadigepalli,Sylvie Ricard-Blum,Renato V Iozzo

American journal of physiology. Cell physiology 324:C893-C909 PubMed36878848

2023

Heparan sulfate proteoglycan in Alzheimer's disease: aberrant expression and functions in molecular pathways related to amyloid-β metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Ilayda Ozsan McMillan,Jin-Ping Li,Lianchun Wang

Development (Cambridge, England) 150: PubMed36779913

2023

A distinct transcriptome characterizes neural crest-derived cells at the migratory wavefront during enteric nervous system development.

Applications

Unspecified application

Species

Unspecified reactive species

Rhian Stavely,Ryo Hotta,Richard A Guyer,Nicole Picard,Ahmed A Rahman,Meredith Omer,Adam Soos,Emoke Szocs,Jessica Mueller,Allan M Goldstein,Nandor Nagy

Nature immunology 24:414-422 PubMed36732425

2023

IFNγ binding to extracellular matrix prevents fatal systemic toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Josephine Kemna,Evelyne Gout,Leon Daniau,Jessica Lao,Kristoffer Weißert,Sandra Ammann,Ralf Kühn,Matthias Richter,Christine Molenda,Anje Sporbert,Dario Zocholl,Robert Klopfleisch,Anja Schütz,Hugues Lortat-Jacob,Peter Aichele,Thomas Kammertoens,Thomas Blankenstein

Journal of inflammation research 16:421-431 PubMed36755970

2023

Protectin DX Relieve Hyperoxia-induced Lung Injury by Protecting Pulmonary Endothelial Glycocalyx.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongjie Liang,Huilin Yue,Congcong Xu,Qian Wang,Shengwei Jin

ACS biomaterials science & engineering 8:2574-2588 PubMed35649243

2022

Peripheral Nerve Decellularization for Extracellular Matrix Hydrogel Use: A Comparative Study.

Applications

Unspecified application

Species

Unspecified reactive species

Emory Gregory,In Ha Baek,Nikolas Ala-Kokko,Reagan Dugan,Luis Pinzon-Herrera,Jorge Almodóvar,Young Hye Song
View all publications
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