Anti-Heparanase 1 antibody [EPR25303-58]
- 20ul selling size
- RabMAb
- Recombinant
- KO Validated
- What is this?
5
(1 Review)
|
(3 Publications)
Rabbit Recombinant Monoclonal Heparanase 1 antibody. Suitable for IP, WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 3 publications.
View Alternative Names
HEP, HPA, HPA1, HPR1, HPSE1, HSE1, HPSE, Heparanase, Endo-glucoronidase, Heparanase-1, Hpa1
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heparanase 1 antibody [EPR25303-58] (AB288438)
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labelling Heparanase 1 with ab288438 at 1/200 (2.07 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on human spleen. The section was incubated with ab288438 at 4°C overnight. Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heparanase 1 antibody [EPR25303-58] (AB288438)
Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labelling Heparanase 1 with ab288438 at 1/200 (2.07 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on human cervical carcinoma. The section was incubated with ab288438 at 4°C overnight. Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heparanase 1 antibody [EPR25303-58] (AB288438)
Immunohistochemical analysis of paraffin-embedded Human thyroid tissue labelling Heparanase 1 with ab288438 at 1/200 (2.07 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Negative control : no staining on human thyroid. The section was incubated with ab288438 at 4°C overnight. Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)
- IP
Supplier Data
Immunoprecipitation - Anti-Heparanase 1 antibody [EPR25303-58] (AB288438)
Heparanase 1 was immunoprecipitated from 0.35 mg Capan-1 (human pancreas adenocarcinoma epithelial cell) whole cell lysate 10 ug with ab288438 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab288438 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Capan-1 (human pancreas adenocarcinoma epithelial cell) whole cell lysate 10 ug
Lane 2 : ab288438 IP in Capan-1 whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab288438 in Capan-1 whole cell lysate
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 3 minutes
All lanes:
Immunoprecipitation - Anti-Heparanase 1 antibody [EPR25303-58] (ab288438)
Predicted band size: 61 kDa
false
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heparanase 1 antibody [EPR25303-58] (AB288438)
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labelling Heparanase 1 with ab288438 at 1/200 (2.07 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on mouse spleen. The section was incubated with ab288438 at 4°C overnight. Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heparanase 1 antibody [EPR25303-58] (AB288438)
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labelling Heparanase 1 with ab288438 at 1/200 (2.07 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on rat spleen. The section was incubated with ab288438 at 4°C overnight. Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heparanase 1 antibody [EPR25303-58] (AB288438)
Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labelling Heparanase 1 with ab288438 at 1/200 (2.07 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Negative control : no staining on mouse pancreas. The section was incubated with ab288438 at 4°C overnight. Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heparanase 1 antibody [EPR25303-58] (AB288438)
Immunohistochemical analysis of paraffin-embedded Mouse pancreatic carcinoma tissue labelling Heparanase 1 with ab288438 at 1/200 (2.07 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on mouse pancreatic carcinoma. The section was incubated with ab288438 at 4°C overnight. Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)
- IP
Supplier Data
Immunoprecipitation - Anti-Heparanase 1 antibody [EPR25303-58] (AB288438)
Heparanase 1 was immunoprecipitated from 0.35 mg B16-F10 (Mouse skin melanoma) whole cell lysate 10 ug with ab288438 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab288438 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : B16-F10 (Mouse skin melanoma) whole cell lysate 10 ug
Lane 2 : ab288438 IP in B16-F10 whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab288438 in B16-F10 whole cell lysate
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 3 minutes
All lanes:
Immunoprecipitation - Anti-Heparanase 1 antibody [EPR25303-58] (ab288438)
Predicted band size: 61 kDa
false
- WB
Lab
Western blot - Anti-Heparanase 1 antibody [EPR25303-58] (AB288438)
Blocking and diluting buffer and concentration : Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBST
Performed under reducing conditions.
Lysates at 20μg per lane.
False colour image of Western blot : Anti-Heparanase 1 antibody [EPR25303-58] (ab288438) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red.
In Western blot, ab288438 was shown to bind specifically to Heparanase 1. Two bands were observed at 50 and 65kDa in wild-type HeLa cell lysates with no signal observed at this size in Heparanase 1 knockout cell line ab265413 (knockout cell lysate ab258456). To generate this image, wild-type and Heparanase 1 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto an immobilon-FL PVDF membrane. Membranes were blocked in Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/10000 dilution.
Negative control : MCF7(PMID : 10395325).
All lanes:
Western blot - Anti-Heparanase 1 antibody [EPR25303-58] (ab288438) at 1/1000 dilution
Lane 1:
Wild-type HeLa (human cervix adenocarcinoma epithelial cell) cell lysate at 20 µg
Lane 2:
HPSE knockout HeLa (human cervix adenocarcinoma epithelial cell) cell lysate at 20 µg
Lane 3:
Capan-1 (human pancreas adenocarcinoma epithelial cell) cell lysate at 20 µg
Lane 4:
HeLa (human cervix adenocarcinoma epithelial cell) cell lysate at 20 µg
Lane 5:
MCF7 (human breast adenocarcinoma epithelial cell) cell lysate at 20 µg
Lane 6:
B16-F10 (Mouse melanoma mixture of spindle-shaped and epithelial-like cells) cell lysate at 20 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/10000 dilution
Predicted band size: 61 kDa
Observed band size: 42 kDa,50 kDa,65 kDa
false
- WB
Lab
Western blot - Anti-Heparanase 1 antibody [EPR25303-58] (AB288438)
Blocking and diluting buffer and concentration : 5% NFDM/TBST
Heparanase 1 is a glycosylated protein and can be deglycosylated by Protein Deglycosylation MIX II.
Exposure time : 3 minutes
Bands : 50, 65, 42 kDa
All lanes:
Western blot - Anti-Heparanase 1 antibody [EPR25303-58] (ab288438) at 1/1000 dilution
Lane 1:
Untreated Capan-1 (human pancreas adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Lane 2:
Capan-1 whole cell lysate treated with Protein Deglycosylation MIX II at 15 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 61 kDa
false
- WB
Lab
Western blot - Anti-Heparanase 1 antibody [EPR25303-58] (AB288438)
Blocking and diluting buffer and concentration : 5% NFDM/TBST The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 10405343, 10395326)
Exposure time : 3 minutes
All lanes:
Western blot - Anti-Heparanase 1 antibody [EPR25303-58] (ab288438) at 1/1000 dilution
All lanes:
Human spleen tissue lysate at 20 µg
Secondary
All lanes:
VeriBlot for IP secondary antibody(HRP)(<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution
Predicted band size: 61 kDa
false
- WB
Lab
Western blot - Anti-Heparanase 1 antibody [EPR25303-58] (AB288438)
Blocking and diluting buffer and concentration : 5% NFDM/TBST
Exposure time : 3 minutes
All lanes:
Western blot - Anti-Heparanase 1 antibody [EPR25303-58] (ab288438) at 1/1000 dilution
All lanes:
Rat spleen tissue lysate at 20 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 61 kDa
false
- WB
Lab
Western blot - Anti-Heparanase 1 antibody [EPR25303-58] (AB288438)
Blocking and diluting buffer and concentration : 5% NFDM/TBST
Low expression : mouse pancreas (PMID : 11406531)
Exposure time : 3 minutes
All lanes:
Western blot - Anti-Heparanase 1 antibody [EPR25303-58] (ab288438) at 1/1000 dilution
Lane 1:
Mouse spleen tissue lysate at 20 µg
Lane 2:
Mouse pancreas tissue lysate at 20 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 61 kDa
false
Related conjugates and formulations (1)
-
Anti-Heparanase 1 antibody [EPR25303-58] - BSA and Azide free
Reactivity data
Product details
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
When heparan sulfate is cleaved by Heparanase 1 it causes the release of HS-bound growth factors and cytokines making them available to cells. This enzymatic activity facilitates cell proliferation and angiogenesis affecting processes important for tissue repair and cancer progression. Heparanase 1 does not require a complex for its function but operates as a monomeric enzyme. Its activity is investigated using assays like heparanase activity assays or ELISA for measuring the enzyme's activity in different biological contexts including mouse models.
Pathways
The activity of Heparanase 1 intersects significantly with the regulation of angiogenesis and cell signaling pathways such as the VEGF and FGF pathways. These pathways are important for new blood vessel formation making them significant in both normal development and cancer. Heparanase 1 interacts with proteins such as VEGF which is important for blood vessel growth and integrins which are involved in cell-matrix interactions and signaling.
Product protocols
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Target data
Publications (3)
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Stem cell research & therapy 16:324 PubMed40597299
2025
Applications
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Carbohydrate polymers 320:121214 PubMed37659814
2023
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The Korean journal of pain 36:60-71 PubMed36536517
2022
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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