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AB314078

Anti-Hepassocin/FGL1 antibody [BLR210K] - BSA free

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Rabbit Recombinant Monoclonal FGL1 antibody. Suitable for ICC, IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human FGL1.

View Alternative Names

HFREP1, FGL1, Fibrinogen-like protein 1, HP-041, Hepassocin, Hepatocyte-derived fibrinogen-related protein 1, Liver fibrinogen-related protein 1, HPS, HFREP-1, LFIRE-1

4 Images
Immunocytochemistry - Anti-Hepassocin/FGL1 antibody [BLR210K] - BSA free (AB314078)
  • ICC

Supplier Data

Immunocytochemistry - Anti-Hepassocin/FGL1 antibody [BLR210K] - BSA free (AB314078)

Detection of human Hepassocin/FGL1 by immunocytochemistry. Sample : FFPE section of SHP-77 cells. Antibody : Rabbit anti-FGL-1 recombinant monoclonal antibody [BLR210K] (A700-210CF). Secondary : HRP-conjugated goat anti-rabbit IgG.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepassocin/FGL1 antibody [BLR210K] - BSA free (AB314078)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepassocin/FGL1 antibody [BLR210K] - BSA free (AB314078)

Detection of human Hepassocin/FGL1 by immunohistochemistry. Sample : FFPE section of liver carcinoma. Antibody : Rabbit anti-FGL-1 recombinant monoclonal antibody [BLR210K] (A700-210CF). Secondary : HRP-conjugated goat anti-rabbit IgG.

Immunoprecipitation - Anti-Hepassocin/FGL1 antibody [BLR210K] - BSA free (AB314078)
  • IP

Supplier Data

Immunoprecipitation - Anti-Hepassocin/FGL1 antibody [BLR210K] - BSA free (AB314078)

Samples : Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hep-G2 cells prepared using NETN lysis buffer. Antibodies : Rabbit anti-Hepassocin/FGL1 recombinant monoclonal antibody [BLR210K] used for IP at 12 µl/mg lysate. Hepassocin/FGL1 was also immunoprecipitated by a second antibody against a different epitope of Hepassocin/FGL1. For blotting immunoprecipitated Hepassocin/FGL1, A700-210 was used at 1 : 1000. Chemiluminescence with an exposure time of 3 seconds.

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Western blot - Anti-Hepassocin/FGL1 antibody [BLR210K] - BSA free (AB314078)
  • WB

Supplier Data

Western blot - Anti-Hepassocin/FGL1 antibody [BLR210K] - BSA free (AB314078)

Samples : Whole cell lysate (10 µg) from HEK293T, SHP-77, HeLa, and Hep-G2 cells prepared using NETN lysis buffer. Antibody : Rabbit anti-Hepassocin/FGL1 recombinant monoclonal antibody [BLR210K] (A700-210 lot 1) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 10 seconds. Lower Panel : Rabbit anti-COPB2 antibody (A304-523A).

All lanes:

Western blot - Anti-Hepassocin/FGL1 antibody [BLR210K] - BSA free (ab314078) at 1/1000 dilution

Lane 1:

HEK293T Whole cell lysate at 10 µg

Lane 2:

SHP-77 Whole cell lysate at 10 µg

Lane 3:

HeLa Whole cell lysate at 10 µg

Lane 4:

Hep-G2 Whole cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR210K

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC, IP, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human FGL1.

Q08830

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
Up to 12 months
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Fibrinogen-like protein 1 (FGL1) also known as Hepassocin plays a significant role in modulating immune responses. FGL1 is a member of the fibrinogen family with a molecular mass of approximately 31 kDa. This protein is widely expressed in the liver and is also found in other tissues like the pancreas and lungs. Its structural homology to fibrinogen suggests potential binding capabilities with similar ligands influencing its mechanical actions in cellular processes.
Biological function summary

FGL1 modulates the immune system and maintains its homeostasis. It functions primarily as a ligand for the immune checkpoint receptor LAG-3. This FGL1-LAG-3 interaction inhibits T-cell activity leading to immune tolerance and helping prevent autoimmunity. FGL1 while not a part of a complex demonstrates significant standalone biological activity by affecting immune responses especially under pathological conditions.

Pathways

FGL1 integrates into the immune regulatory framework. It exerts its effects mainly through the immune checkpoint pathway impacting T-cell regulation. In addition to LAG-3 FGL1 interacts closely with the PD-1/PD-L1 pathway which is also important in checkpoint inhibition and immune evasion mechanisms. These pathways together orchestrate the immune surveillance system and play roles in maintaining immune equilibrium.

FGL1 has received attention in cancer particularly in its role of suppressing anti-tumor immunity. Its interaction with LAG-3 makes it a potential target for cancer immunotherapy as high FGL1 levels correlate with poor prognosis in various cancers. Additionally it has implications in autoimmune diseases where its regulation of T-cell activity prevents excessive immune responses. By modulating LAG-3 activity FGL1 emerges as a therapeutic target of interest in autoimmune conditions presenting new opportunities for managing such disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Immune suppressive molecule that inhibits antigen-specific T-cell activation by acting as a major ligand of LAG3 (PubMed : 30580966). Responsible for LAG3 T-cell inhibitory function (PubMed : 30580966). Binds LAG3 independently from MHC class II (MHC-II) (PubMed : 30580966). Secreted by, and promotes growth of, hepatocytes (PubMed : 11470158, PubMed : 19880967).
See full target information FGL1
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Product promise

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