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AB277481

Anti-Hepatitis B Surface Antigen antibody [5C3]

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Mouse Monoclonal Hepatitis B Surface Antigen (Adw) antibody. Suitable for IHC-P and reacts with Hepatitis B virus samples.

View Alternative Names

S gene subtype adw2, sAg, ssAg

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepatitis B Surface Antigen antibody [5C3] (AB277481)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepatitis B Surface Antigen antibody [5C3] (AB277481)

Formalin-fixed, paraffin-embedded HBV-infected human liver stained for Hepatitis B Surface Antigen using ab277481 at 1/25 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepatitis B Surface Antigen antibody [5C3] (AB277481)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepatitis B Surface Antigen antibody [5C3] (AB277481)

Immunohistochemical analysis (Formalin-fixed, paraffin-embedded) of HBV-infected human liver stained for Hepatitis B Surface Antigen using ab277481 at 1/25 dilution. Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Counterstained with hematoxylin.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5C3

Isotype

IgG2a

Light chain type

kappa

Carrier free

No

Reacts with

Hepatitis B virus

Applications

IHC-P

applications

Specificity

This antibody stains the cytoplasm of antigen-positive liver cells in patients with Type B viral hepatitis. This antibody reacts with the 'a' determinant present on the HBsAg subtypes ayw1, ayw2, ayw3, ayw4, ayr, adw2, adw4 and adr. It does not react with normal tissues.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Hepatitis B virus": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/25 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.4 - 7.6 Preservative: 0.1% Sodium azide Constituents: 98.9% Tris buffer, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Hepatitis B Surface Antigen also known as HBsAg is a protein associated with the hepatitis B virus (HBV). It has a molecular weight of approximately 24 kDa. HBsAg expresses itself on the surface of HBV particles and infected liver cells. The protein exists in various forms including spherical and filamentous particles. Expression of HBsAg serves as a marker of active HBV infection and is a target for immune response.
Biological function summary

The Hepatitis B Surface Antigen facilitates the virus's entry into hepatocytes by attaching to the host cell receptors. The antigen is known to associate with a protein complex on the viral envelope that plays a role in the virus's infectivity and replication efficiency. The presence of HBsAg in the bloodstream signifies an ongoing infection and can stimulate the production of antibodies hepatitis B which aim to neutralize the virus and clear infection.

Pathways

Hepatitis B surface antigen plays a critical role in the immune evasion strategies of hepatitis B virus. It modulates key pathways such as the host's innate immune response affecting interferon signaling. HBsAg interacts with the hepatocyte receptor NTCP during the viral lifecycle which allows for the successful entry and subsequent infection of liver cells. NTCP’s involvement highlights the interconnectedness of how HBV exploits host cellular pathways to propagate.

The hepatitis B surface antigen is primarily associated with hepatitis B infection and its related complications such as chronic hepatitis B and hepatocellular carcinoma. Chronic presence of HBsAg in the bloodstream indicates active infection increasing the risk of liver damage over time. This condition often involves liver proteins such as alpha-fetoprotein which is frequently elevated in hepatocellular carcinoma illustrating the progression from hepatitis B infection to serious liver disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Hepatitis B Surface Antigen (Adw)
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