AB8639

Anti-Hepatitis B Virus Core Antigen antibody [10E11]

Name: Anti-Hepatitis B Virus Core Antigen antibody [10E11] (ab8639) | Abcam
Brand: Abcam Limited
SKU: AB8639

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(22 Publications)

Anti-Hepatitis B Virus Core Antigen antibody [10E11] (ab8639) is a mouse monoclonal antibody detecting Hepatitis B Virus Core Antigen in Western Blot, IP, IHC-P, IHC-Fr, ICC/IF, ELISA. Suitable for Hepatitis B virus.

- Over 10 publications
- Trusted since 2002

View Alternative Names

Capsid Protein, Core and e antigen, Core antigen, Core protein, HBVgp4, HBc, HBcAg, Hepatitis B Virus core antigen, Hepatitis B core antigen, p21.5, precore/core protein

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

10E11

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Hepatitis B virus

Applications

ICC/IF, IHC-Fr, ELISA, IP, WB, IHC-P

applications

Epitope

aa positions 1-10

Specificity

This antibody reacts with HBV Core Antigen (amino acid residues 1-10). Ab8639 should recognize both the precoreprotein and core protein.

Ab8639 was raised against serotype ayw but will work with all other genotypes.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Hepatitis B virus": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-Hepatitis B Virus Core Antigen antibody [10E11] (ab8639) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Hepatitis B virus samples.

Trusted by the scientific community
Anti-Hepatitis B Virus Core Antigen [10E11] (ab8639) was first used in a scientific publication in 2002 and has been cited over 10 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purification technique

Diafiltration

Purification notes

The bioreactor harvest was dialyzed against PBS, pH 7.4, BSA was added, and the prep was filter-sterilized

Storage buffer

pH: 7.4 Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Hepatitis B Virus Core Antigen also known as HBV core antigen or Hep B core is an important component of the hepatitis B virus (HBV). This antigen often referred to simply as 'core' forms the nucleocapsid of the virus. The HBV core antigen consists of a protein structure with a mass of approximately 21 kilodaltons. It is primarily expressed within the hepatocytes the liver cells where it plays a pivotal role in viral replication and assembly. The HBV core antigen is also referred to in studies as HBV protein or B core.

Biological function summary

The hepatitis B core antigen is integral for the assembly of the viral capsid which protects the viral genome during infection. It functions as part of a larger complex with other viral proteins including the pre-core and envelope proteins to facilitate viral lifecycle processes. This antigen allows for the encapsidation of viral RNA and serves as a scaffold for reverse transcription of the viral genome ensuring replication fidelity and successful infection spread.

Pathways

The hepatitis B core antigen is essential in the viral replication pathway and the HBV lifecycle pathway. It interacts closely with HBV polymerase during the packaging of viral genome into the capsid. These interactions are important for the transformation of RNA into DNA a critical step in viral maturation. The antigen is related to the HBx protein through these pathways; the HBx protein modulates viral replication and can influence the pathogenesis of the virus.

The hepatitis B core antigen is strongly linked to chronic hepatitis B infection and liver diseases such as cirrhosis and hepatocellular carcinoma. The persistence of the core antigen in infected hepatocytes signifies ongoing viral replication which can lead to liver inflammation and damage. The interaction of the core antigen with immune system components such as anti-HBc antibodies assists in the diagnosis and monitoring of disease progression. It connects to the envelope proteins during the disease process which are important in immune system evasion and chronic infection establishment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Hepatitis B Virus Core Antigen

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Publications (22)

Recent publications for all applications. Explore the full list and refine your search

Acta biochimica et biophysica Sinica 57:486-496 PubMed39722572

2024

The host gene regulates HBV replication via HBV PRE-induced nuclear export.

Applications

Unspecified application

Species

Unspecified reactive species

Jinyu Wang,Jing Li,Wentao Xie,Zhongliang Shen,Jingwen Wu,Richeng Mao,Mengji Lu,Jiming Zhang

iMeta 3:e221 PubMed39135698

2024

Cell-type-specific expression analysis of liver transcriptomics with clinical parameters to decipher the cause of intrahepatic inflammation in chronic hepatitis B.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Wang,Qian Li,Yuanwang Qiu,Simo Kitanovski,Chen Wang,Chenxia Zhang,Fahong Li,Xiaoguang Li,Zhenfeng Zhang,Lihua Huang,Jiming Zhang,Daniel Hoffmann,Mengji Lu,Hongzhou Lu

Microbiology spectrum 12:e0136523 PubMed38315030

2024

Hepatitis B virus-targeting sodium taurocholate cotransporting polypeptide mediates HBV infection and damage in human renal podocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Lifen Wang,Cheng Wang,Xu Wang,Yantao Cao,Xiaohua Guo,Zhiming Ye

Journal of virology 97:e0072223 PubMed37754761

2023

Novel non-HAP class A HBV capsid assembly modulators have distinct and profiles.

Applications

Unspecified application

Species

Unspecified reactive species

Hannah Vanrusselt,Dieudonné Buh Kum,Valerio Taverniti,Cheng Liu,Abel Acosta Sanchez,Nikky Corthout,Sebastian Munck,Thomas F Baumert,Leonid Beigelman,Lawrence M Blatt,Julian A Symons,Jerome Deval,Pierre Raboisson,Eloi R Verrier,Andreas Jekle,Sandrine Vendeville,Yannick Debing

PLoS pathogens 18:e1010204 PubMed35030230

2022

Bay41-4109-induced aberrant polymers of hepatitis b capsid proteins are removed via STUB1-promoted p62-mediated macroautophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Jiacheng Lin,Limin Yin,Xia-Zhen Xu,He-Chen Sun,Zhi-Hua Huang,Xue-Yun Ni,Yan Chen,Xu Lin

Science progress 104:368504211058036 PubMed34825857

2021

NLRX1 can counteract innate immune response induced by an external stimulus favoring HBV infection by competitive inhibition of MAVS-RLRs signaling in HepG2-NTCP cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Jiao,Wenxiong Xu,Xiaoyan Guo,Huiyuan Liu,Baolin Liao,Xiang Zhu,Chuming Chen,Fangji Yang,Lina Wu,Chan Xie,Liang Peng

Vaccines 9: PubMed33669126

2021

-Based Cell-Free Protein Synthesis for Iterative Design of Tandem-Core Virus-Like Particles.

Applications

Unspecified application

Species

Unspecified reactive species

Noelle Colant,Beatrice Melinek,Stefanie Frank,William Rosenberg,Daniel G Bracewell

Scientific reports 10:17095 PubMed33051543

2020

Covalent protein display on Hepatitis B core-like particles in plants through the in vivo use of the SpyTag/SpyCatcher system.

Applications

Unspecified application

Species

Unspecified reactive species

Hadrien Peyret,Daniel Ponndorf,Yulia Meshcheriakova,Jake Richardson,George P Lomonossoff

Molecular and cellular biology 40: PubMed31712392

2020

Regulation of Molecular Chaperone GRP78 by Hepatitis B Virus: Control of Viral Replication and Cell Survival.

Applications

Unspecified application

Species

Unspecified reactive species

Wangqin Shu,Zhiwei Guo,Lijie Li,Zhiqi Xiong,Ziyu Wang,Yuanyuan Yang,Yuqi Li,Minjing He,Ruijie Gong,Bo Gao

Frontiers in microbiology 10:2869 PubMed31921048

2019

Host Gene SEL1L Involved in Endoplasmic Reticulum-Associated Degradation Pathway Could Inhibit Hepatitis B Virus at RNA, DNA, and Protein Levels.

Applications

Unspecified application

Species

Unspecified reactive species

Jinyu Wang,Jing Li,Jingwen Wu,Minhui Dong,Zhongliang Shen,Yong Lin,Fahong Li,Yongmei Zhang,Richeng Mao,Mengji Lu,Jiming Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Hepatitis B Virus Core Antigen antibody [10E11]

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Epitope

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (22)

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