Anti-Hepatitis B Virus Core Antigen antibody [EPR28251-34]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepatitis B Virus Core Antigen antibody [EPR28251-34] (AB316283)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Hepatitis B Virus Core Antigen with ab316283 at 1/2000 (0.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on human cerebrum. The section was incubated with ab316283 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepatitis B Virus Core Antigen antibody [EPR28251-34] (AB316283)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Hepatitis B Virus Core Antigen with ab316283 at 1/2000 (0.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on rat cerebrum. The section was incubated with ab316283 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepatitis B Virus Core Antigen antibody [EPR28251-34] (AB316283)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Hepatitis B Virus Core Antigen with ab316283 at 1/2000 (0.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse cerebrum. The section was incubated with ab316283 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Hepatitis B Virus Core Antigen antibody [EPR28251-34] (AB316283)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / 293T cells (human embryonic kidney epithelial cell) transfected with a Virus wild-type Hepatitis B Virus Core Antigen expression vector containing a myc-His-tag®(Middle) / 293T cells transfected with an empty expression vector containing a myc-His-tag® (Right) cells labelling Hepatitis B Virus Core Antigen with ab316283 at 1/500 dilution (0.1 ug)/Middle and Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells are co-stained with Myc tag conjugated to Alexa Fluor®647.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepatitis B Virus Core Antigen antibody [EPR28251-34] (AB316283)

Immunohistochemical analysis of paraffin-embedded (A) 293T cells transfected with a Virus wild-type Hepatitis B Virus Core Antigen expression vector containing a myc-His-tag® (B) 293T cells (human embryonic kidney epithelial cell) transfected with an empty vector containing a myc-His-tag® tissue labeling Hepatitis B Virus Core Antigen with ab316283 at 1/2000 (0.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on 293T cells transfected with a Virus wild-type Hepatitis B Virus Core Antigen expression vector containing a myc-His-tag® (image A). No staining on 293T cells transfected with an empty vector containing a myc-His-tag® (image B). The section was incubated with ab316283 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Hepatitis B Virus Core Antigen antibody [EPR28251-34] (AB316283)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Hepatitis B Virus Core Antigen with ab316283 at 1/100 (4.99 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing positive staining in 293T cells transfected with a Virus wild-type Hepatitis B Virus Core Antigen expression vector containing a myc-His-tag®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain at 1/100 (5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

• I-ELISA

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Indirect ELISA - Anti-Hepatitis B Virus Core Antigen antibody [EPR28251-34] (AB316283)

Indirect ELISA analysis of ab316283 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution.

Antigen : Hepatitis B Virus Core Antigen peptide a, Hepatitis B Virus Core Antigen peptide b.

Antigen concentration : 1000 ng/ml

• WB

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Western blot - Anti-Hepatitis B Virus Core Antigen antibody [EPR28251-34] (AB316283)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Hepatitis B Virus Core Antigen antibody [EPR28251-34] (ab316283) at 1/1000 dilution

Lane 1:

293T cells (human embryonic kidney epithelial cell) transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a Virus HBEAG_HBVA7 (genotype A7) vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a Virus CAPSD_HBVB1 (genotype B1) vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a Virus CAPSD_HBVC5 (genotype C5) vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 5:

293T cells transfected with a Virus CAPSD_HBVE1 (genotype E1) vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 6:

293T cells transfected with a Virus HBEAG_HBVD3 (genotype D3) vector containing a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 25 kDa,36 kDa

false

Exposure time: 8s

• WB

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Western blot - Anti-Hepatitis B Virus Core Antigen antibody [EPR28251-34] (AB316283)

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Hepatitis B Virus Core Antigen antibody [EPR28251-34] (ab316283) at 1/1000 dilution

Lane 1:

293T cells (human embryonic kidney epithelial cell) transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a Virus wild-type Hepatitis B Virus Core Antigen expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 25 kDa,124 kDa

false

Exposure time: 6s